17 results about "MANGIFERA INDICA LEAF" patented technology

The invention belongs to the technical field of agricultural microorganisms, and relates to a bacillus velezensis strain S-34 and application thereof in prevention and treatment of mango leaf anthracnose, the bacillus velezensis strain S-34 is separated from rhizosphere soil of a mango variety 'Jinhuang' with high anthracnose resistance, is preserved in China Center for Type Culture Collection on May 29, 2025, and has a preservation number of CCTCC No. M 20251225. The bacillus velezensis S-34 provided by the invention not only can be used for preventing and treating mango leaf anthracnose, but also has a good antagonistic effect on various mango leaf pathogenic fungi. In addition, the bacillus velezensis S-34 as well as sterile fermentation liquor and volatile gas of the bacillus velezensis S-34 can inhibit spore germination and epidermis penetration processes of the colletotrichum siamensis, so that the infection process of the colletotrichum siamensis on plants is inhibited. The invention provides a novel antibiological inoculant and a preparation method for efficient prevention and treatment of mango leaf anthracnose and other leaf diseases in agricultural production, and is beneficial to green and healthy development of the mango industry.

The invention belongs to the field of mango cultivation, and specifically relates to a composition for increasing the wax content of mango leaf epidermis and its application. The invention uses melatonin (MT) and palmitic acid (PA) in combination, and after spraying mango leaves, the wax content on the surface of 'Tainong No. 1' mango leaves can be significantly increased, and the composition of mango leaf wax can also be affected, so as to affect the resistance of mango leaves to biological and non-biological stresses, and provide a reference for the synthetic regulation of mango leaf epidermal wax.

The invention discloses a specific PCR (Polymerase Chain Reaction) detection primer IKF5 / IKR4 of Karst colletotrichum gloeosporioides and application of the specific PCR detection primer IKF5 / IKR4, the specific detection primers IKF5: 5 '-GTAGGCCGTCCCCTGAAAAG-3' and IKR4: 5 '-CCAAGCAGCTTGAGGGTT-3' on the basis of ITS (International Transcription Sequence) sequences, and the sensitivity of the specific detection primers IKF5: 5 '-GTAGGCCGTCCCTGAAAAG-3' and IKR4: 5 '-CCAAGCAGCTTGAGGGTT-3' is 100 pg. Mu L <-1 >. Research results show that the pair of primers can efficiently and specifically detect the Karst colletotrichum in the mango leaves, and a reliable technical tool is provided for early diagnosis of mango anthracnose, analysis of a disease occurrence rule and construction of a precise prevention and control strategy.

This invention belongs to the field of agricultural microbiology technology and relates to a strain of Bacillus velezensis S-34 and its application in controlling mango leaf anthracnose. It was isolated from the rhizosphere soil of the anthracnose-resistant mango variety 'Jinhuang' and deposited at the China Center for Type Culture Collection (CCTCC) on May 29, 2025, with accession number CCTCC No. M 20251225. The Bacillus velezensis S-34 provided by this invention not only controls mango leaf anthracnose but also exhibits good antagonistic effects against various mango leaf pathogenic fungi. Furthermore, Bacillus velezensis S-34, its sterile fermentation broth, and volatile gases can inhibit the germination and epidermal penetration of Anthracnose spores, thereby inhibiting the infection process of Anthracnose spores on plants. This invention provides a new biocontrol agent and preparation method for the efficient control of mango leaf anthracnose and other foliar diseases in agricultural production, which is beneficial to the green and healthy development of the mango industry.

The invention relates to a mango leaf plant source aggregation-induced fluorescence carbon quantum dot preparation method, which comprises: 1, selecting mango leaves as a raw material, freeze-drying, and crushing to obtain mango leaf powder; and 2, carrying out heating reflux on the mango leaf powder obtained in the step 1 and an absolute ethyl alcohol-water mixed aqueous solution in a dark place to obtain a carbon quantum dot solution, and then carrying out dialysis and freeze drying to obtain the carbon quantum dots. The plant-derived AIE type carbon quantum dots with solid fluorescence performance are prepared by taking mango leaves as a raw material, the traditional aggregation fluorescence quenching effect of CDs is effectively solved, and the AIE type carbon quantum dots have high AIE-CDs fluorescence efficiency.

The invention discloses a specific PCR (Polymerase Chain Reaction) detection primer TKF10 / TKR10 of Karst colletotrichum gloeosporioides and an application of the specific PCR detection primer TKF10 / Wherein the sensitivity of primers TKF10: 5 '-GCGGGGCTAACTCTGAAT-3' and TKR10: 5 '-CGCCAGAGATGTTTGCCTG-3' based on a TUB2 (beta-tubulin) gene is 1 ng * [mu] L <-1 >, and the sensitivity of the primers TKF10: 5 '-GCGGGGCTAACTCTGAAT-3' and TKR10: 5 '-CGCCAGAGATGTTTGCCTG-3' based on the TUB2 (beta-tubulin) gene is 1 ng * [ Research results show that the pair of primers can efficiently and specifically detect the Karst colletotrichum in the mango leaves, and a reliable technical tool is provided for early diagnosis of mango anthracnose, analysis of a disease occurrence rule and construction of a precise prevention and control strategy.

The invention provides a method for extracting microcrystalline cellulose by taking mango leaves as a raw material, and belongs to the technical field of microcrystalline cellulose preparation, the method comprises the following steps: cleaning mango leaves, drying, mixing with chloroform, carrying out heat treatment, dewaxing, and crushing; carrying out alkali treatment and acid hydrolysis on the dewaxed raw material to remove lignin and hemicellulose in the raw material; mixing a product obtained after acid-base hydrolysis with a sodium hypochlorite solution, and performing heat treatment bleaching; mixing the bleached product with a hydrogen peroxide solution for heat treatment to improve the crystallinity of the product; and spin-drying the material after the reaction is finished, washing the material with water until the material is neutral, and then spin-drying, freeze-drying and ball-milling and crushing the material to obtain the microcrystalline cellulose product. According to the method for extracting the microcrystalline cellulose from the mango leaves, provided by the invention, by optimizing dewaxing, delignification, acid hydrolysis and bleaching processes and combining a mild crystallinity enhancing step, efficient preparation of the microcrystalline cellulose with high purity and high crystallinity is realized.

The invention belongs to the field of mango cultivation, and particularly relates to a composition for improving epidermis wax of mango leaves and application of the composition. The melatonin (MT) and the palmitic acid (PA) are combined for use, and after the melatonin (MT) and the palmitic acid (PA) are sprayed on the mango leaves, the wax content of the surfaces of the mango leaves' Taiong No.1 'can be remarkably increased, meanwhile, the composition of the wax of the mango leaves can be influenced, the mango leaves are influenced to resist biological and abiotic stress, and a reference is provided for the synthetic regulation effect of the wax on the epidermis of the mango leaves.

The invention discloses a traditional Chinese medicine composition for promoting growth and improving meat quality of meat rabbits as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition comprises the following components in parts by weight: 5-20 parts of astragalus membranaceus, 5-20 parts of liquorice, 5-30 parts of codonopsis pilosula, 10-30 parts of folium mori, 5-30 parts of acanthopanax senticosus leaves and 5-20 parts of mango leaves. The composition is convenient to feed, can enhance the immunity of the meat rabbits, reduces the morbidity of the meat rabbits, effectively promotes food digestion and absorption of the meat rabbits, improves the growth performance of the meat rabbits, improves the meat quality of the meat rabbits, and is low in cost.

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition and traditional Chinese medicine gel for treating gynecological vaginitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from sugarcane leaves, mango leaves, cinnamon leaves, citronella, gallnut, cortex phellodendri, rhizoma atractylodis, radix rubiae, fructus cnidii, pericarpium granati, radix sophorae flavescentis and alums. The traditional Chinese medicine gel comprises a traditional Chinese medicine composition and a gel matrix (Carbomer-940 and glycerol). The traditional Chinese medicine composition is subjected to water extraction and prepared into the gel, and the obtained traditional Chinese medicine gel has the effects of clearing away heat and drying dampness and killing parasites to relieve itching and is suitable for gynecological vagina inflammation, vulva infection and other symptoms. The traditional Chinese medicine composition can effectively prolong the duration of the medicine at the medication part, is beneficial to improving the medication compliance of a patient, can strengthen the treatment effect, and plays a key role in improving the compliance of the patient and enhancing the treatment effect of the medicine.

The present invention relates to the field of traditional Chinese medicine, and in particular to a Chinese medicine composition for resisting muscle atrophy and a preparation process for enhancing muscle regeneration. The present invention comprises a Chinese medicine composition comprising 12-15 parts by weight of astragalus, 6-8 parts of ginseng, 5-10 parts of julibrissin bark, 6-8 parts of cyathulae, 3-6 parts of pollen, 5-8 parts of curcuma, 3-8 parts of lily, 3-8 parts of white peony root, and 3-8 parts of mango leaf. The technical scheme of the present invention can be used to improve the mental state of patients while resisting muscle atrophy.

The invention provides a method for extracting and preparing mangiferin from Guiqiangmangifera indica leaves, which comprises the following steps: firstly, quickly freezing by liquid nitrogen to destroy cell wall structures and improve the permeability of a solvent, then treating mango leaf powder by compound enzyme to break compact cell walls, and extracting by using a deep eutectic solvent prepared from betaine, lactic acid group and ethanol to obtain mangiferin. According to the method, the mangiferin is selectively dissolved, the viscosity is reduced, the diffusion rate is increased, efficient extraction of the mangiferin is achieved, meanwhile, solvent recycling is achieved, the adopted raw materials are natural substances easy to decompose, pollution to the environment is reduced, finally, continuous purification is achieved through gradient membrane separation, use of organic solvents is reduced, and the cost is reduced. Therefore, the purity of the mangiferin product is improved.

The application provides a postharvest preservation method of mango, which comprises the following steps: S1. picking and pretreatment, S2. short-wave ultraviolet mild irradiation treatment, S3. preservative solution immersion and storage. The preservative solution is composed of phyllium extract solution, mango leaf extract solution, arginine grafted modified chitosan, glacial acetic acid and sterile water. Through the synergistic process of the short-wave ultraviolet mild irradiation and the preservative solution immersion, combined with the multiple effects of the modified chitosan coating and the plant extract, the mango can be stored for 30 days under the condition of 13-15 DEG C and 85-90% relative humidity, the good fruit rate is still kept to be more than 90%, and the weight loss rate is less than 5%, so that the industry problems that the mango is prone to rot and fast water loss after harvesting are effectively solved. The components of the preservative solution used in the application are all safe natural sources, no chemical synthetic fungicides are used in the whole process, there is no pesticide residue risk, and the green preservation and food safety requirements are met.

The invention provides clam cassia bark and poria cocos stasis removing pills as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine composition is prepared from the following raw material components in parts by mass: 10 to 20 parts of clam shells, 20 to 30 parts of oysters, 8 to 12 parts of cassia twigs, 10 to 20 parts of poria cocos, 10 to 20 parts of spica prunellae, 10 to 15 parts of semen litchi, 10 to 15 parts of pericarpium citri reticulatae, 8 to 12 parts of rhizoma pinellinae praeparata, 10 to 20 parts of mango leaves, 10 to 15 parts of prismatomeris tetrandra, 1 to 5 parts of leeches, 25 to 35 parts of radix fici simplicissimae and 10 to 15 parts of rhizoma atractylodis macrocephalae. The traditional Chinese medicine composition provided by the invention can be used for treating nodular goiter.

The invention provides a plant-based efficient water body nitrite bio-adsorbent and a preparation method thereof. Compared with a traditional bio-adsorbent, the plant-based efficient water body nitrite bio-adsorbent prepared through the method has the advantages that the specific surface area is larger, nitrite in a water body can be effectively removed, and gt can be achieved through the five raw materials including the azadirachta indica leaves, the fructus crataegi leaves, the guava leaves, the orange peels and the mango leaves; the adsorption effect of the biological adsorbent prepared from the annona squamosa leaves and the fructus crataegi leaves on nitrite in sewage can reach 100%, the biological adsorbent is selected from branches, leaves or epidermis of the five plants, the raw materials are extremely low in cost, diversified in source and easy to obtain, and the biological adsorbent can be obtained from daily collected fallen leaves and household garbage; and efficient resource utilization of the household garbage is achieved, and good economic and social benefits are achieved.

The invention relates to the technical field of gene agriculture, in particular to a mango U6 promoter and application thereof. According to the application, seven U6 promoter sequences are screened from a mango genome by utilizing a conserved sequence of an arabidopsis thaliana U6 promoter on the basis of genome data of'miscanthus spp ', MiU6-2P-1, MiU6-6P-1, MiU6-6P-3 and MiU6-7P-2 in the seven MiU6 promoters successfully drive a GUS reporter gene to be expressed in the arabidopsis thaliana, the MiU6-6P-3 has the highest transcriptional activity, and then the MiU6-7P-2 and the MiU6-2P-1 have the highest transcriptional activity. Furthermore, transient expression is carried out in transgenic tobacco and mango leaves, and detection finds that the GUS activity of the MiU6-6P-3 and the MiU6-7P-2 is relatively high. In combination with GUS activity detection of the three different transgenic plants, MiU6-6P-3 is preferentially recommended to be used as a U6 promoter, and then MiU6-7P-2 is recommended to be used. The research provides a candidate U6 promoter for editing mangoes by using a CRISPR / Cas9 genome editing technology.

The present invention is related to the induction of stomatai opening in order to bypass the impaired stomatai opening in the cold-stress mango leaves, reduce leaf cold damage, and improve the overall physiological state of the plant. The composition of the present invention is useful for preventing or treating cold-night-bright-day stress damage in tropical and subtropical trees, especially mango trees. The said composition comprising at least one stomatai opening inducer substance, and a surfactant. The invention is also related to a method of preventing cold-night-bright-day stress damage in tropical and subtropical trees, wherein said method comprises steps of: (a) foliar spray or cotton swab treatment with the compositions disclosed herein; (b) wherein the foliar spray or cotton swab is applied on the following lighted-day just before the sunrise; and (c) inducing the stomatai opening.