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Fungicide composition and application thereof

A composition and aqueous solution technology, applied in the direction of application, bactericide, biocide, etc., can solve the problems of increased drug resistance of pathogenic bacteria, impact on food safety, environmental pollution, etc.

Active Publication Date: 2020-07-31
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abuse of high concentrations of chemical fungicides can easily lead to increased resistance of pathogenic bacteria and environmental pollution, seriously affecting food safety

Method used

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  • Fungicide composition and application thereof
  • Fungicide composition and application thereof
  • Fungicide composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Determination of the antibacterial activity of 2-phenylethanol to mango leaf spot

[0022] Cultivate the mango stalk rot pathogen Botryosphaeria dothidea YB11-2 (Botryosphaeria dothidea) on a PDA plate, and after 3 days at room temperature, use a sterilized 5mm puncher to punch out the bacteria cake at the edge of the plate colony. Measure 20ml of melted PDA medium with a sterilized graduated cylinder, add 1ul, 10ul, 100ul, 1000ul of 2-phenylethanol respectively, mix evenly and pour it into a plate with a diameter of 9cm, and make 2-phenylethanol concentrations of 1ul / 20ml, 10ul / 20ml, 100ul / 20ml, 1000ul / 20ml PDA plate. The pathogenic bacteria cake with a diameter of 5 mm is transferred to the center of the plate with a sterilized toothpick, and cultivated in a 25 degree Celsius incubator. The pathogenic bacteria cultured without adding 2-phenylethanol was used as the control, and each treatment was repeated three times. When the petri dish was covered with ...

Embodiment 2

[0025] Example 2 The bacteriostatic activity assay of 2-phenylethanol to 11 kinds of pathogenic fungi

[0026] Select 11 kinds of pathogenic bacteria isolated from mango leaf spot (Table 1) and culture them on PDA plates. After culturing at room temperature for 3 days, use a sterilized 5mm puncher to punch bacteria cakes from the edge of the plate colonies. Measure 20ml of melted PDA medium with a sterilized graduated cylinder, add 1ul, 10ul, 100ul, 1000ul 2-phenylethanol respectively, mix well and pour into a plate with a diameter of 9cm to make 2-phenylethanol concentrations of 1ul / 20ml, 10ul / 20ml, 100ul / 20ml, 1000ul / 20ml plates. Transfer the pathogenic bacteria cake to the center of the plate with a sterilized toothpick, and cultivate it in an incubator at 25 degrees Celsius. Take the pathogenic bacteria cultivated without adding 2-phenylethanol as the control, and when the control group is full of petri dishes, use the cross method to measure the pathogenic bacteria colo...

Embodiment 3

[0032] Example 3 Control experiment of 2-phenylethanol mixed with prochloraz manganese salt on mango leaf spot

[0033] Prepare 5ml / l 2-phenylethanol respectively, two kinds of medicinal liquids of 1g / L prochloraz manganese salt are used as mother liquor, and mix in different ratios (9:1, 8:2, 7:3, 6:4, 5: 5, 4:6, 3:7, 2:8, 1:9) Select healthy mango leaves without obvious diseases and soak them in two kinds of mother solutions and mixed medicinal solutions in different proportions for 5 minutes. Use a sterilized 5mm hole puncher to punch out the bacteria cake on the edge of the YB11-2 plate colony. Use a sterilized toothpick to puncture mango leaves six times, and the wound is roughly circular. Use a sterilized toothpick to inoculate the bacteria cake into the puncture site, and the control group is inoculated with a blank agar block. The inoculated mango leaves were placed in plastic boxes with covers soaked with sterile water, and 6 mango leaves were placed in each plastic ...

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Abstract

The invention relates to a fungicide composition and application thereof, and belongs to the field of plant control. According to the invention, 2-phenethyl alcohol and prochloraz-manganese chloride complex are mixed to prevent and treat mango leaf spot; it is found that the control effects of 50%-70% of a 2-phenethyl alcohol aqueous solution having a concentration of 5g / L and 30%-50% of a prochloraz-manganese chloride complex aqueous solution having a concentration of 1g / L are remarkably higher than those of single agent treatment, and the control effect of the composition is improved by 3%-21% compared with that of prochloraz-manganese chloride complex and improved by 112%-193% compared with that of 2-phenethyl alcohol. The 2-phenethyl alcohol and the prochloraz-manganese chloride complex are mixed, so that the control effect can be remarkably improved, the dosage of the prochloraz-manganese chloride complex is reduced, and pesticide reduction and synergism are realized.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a fungicidal composition and its application in the prevention and control of mango plant diseases. Background technique [0002] 2-phenylethanol (Phenylethyl alcohol) is a kind of aromatic alcohol with rose aroma, which widely exists in nature, such as fresh fruits (such as tomatoes, apples, bananas, grapes, etc.), flowers (such as roses) and yeasts (such as Saccharomyces cerevisiae ), and is the main aroma component of cheese, bread, beer, wine, cocoa, coffee and other foods (Fang Jingfan. Study on the effect of phenylethyl alcohol on Penicillium citrus and biocontrol bacteria 34-9[D]. Huazhong Agricultural University, 2013.). As a food additive, 2-phenylethanol has obtained the safety certification of the FDA (Food and Drug Administration) in the United States, and can be used in artificial flavors at a concentration of 2 mg / kg in Europe. 2-phenylethanol is widely used in dai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N47/38A01N31/04A01P3/00
CPCA01N31/04A01N47/38
Inventor 李其利赵江唐丽华黄穗萍郭堂勋余知和莫贱友
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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