Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for extracting high-purity mangiferin from mango leaves

A technology of mango leaves and mangiferin, which is applied in the field of extraction of plant functional components, can solve the problems of low solubility of mangiferin, lower extraction rate, and low extraction rate, and achieve the effects of low cost, increased yield, and high extraction rate

Active Publication Date: 2022-02-01
湖北博瑞生物科技股份有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method utilizes alkaline aqueous solution to extract mangiferin, which causes the solubility of mangiferin in the solvent to be low and reduces the extraction rate.
[0005] In summary, the current technical solutions for extracting mangiferin from mango leaves have problems such as poor safety or low extraction rate.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for extracting high-purity mangiferin from mango leaves
  • A method for extracting high-purity mangiferin from mango leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0049] 1) Take mango leaves with complete leaf shape and good elasticity, wash, drain, remove petiole, dry at 40°C low temperature, crush and pass through 80-mesh sieve, and store in dark place for later use;

[0050] 2) Mix the treated mango leaves with 75% ethanol solution at a ratio of 1:5g / mL, heat to 40°C with microwaves, and perform ultrasonic extraction twice, with extraction power of 120w and extraction time of 15min;

[0051] 3) Combine the extracts, centrifuge the extracts at 4°C and 3500r / min for 15min, and take the supernatant;

[0052] 4) The supernatant was rotary evaporated at 45°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0053] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 12BV, and after the purification was completed, it was eluted with 75% ethano...

Embodiment 2

[0057] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0058] 1) Take the mango leaves with complete leaf shape and good elasticity, wash, drain, remove the petiole, dry at 40°C, pass through a 120-mesh sieve after crushing, and store in the dark for later use;

[0059] 2) Mix the treated mango leaves with 95% ethanol solution at a ratio of 1:15g / mL, heat to 60°C with microwave, and perform ultrasonic extraction 5 times respectively, with extraction power of 120w and extraction time of 45min;

[0060] 3) Combine the extracts, centrifuge the extracts at 0°C and 5000r / min for 45min, and take the supernatant;

[0061] 4) The supernatant was rotary evaporated at 60°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0062] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 9BV, and after the purification was completed, it was eluted wit...

Embodiment 3

[0066] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0067] 1) Take mango leaves with complete leaf shape and good elasticity, wash, drain, remove the petiole, dry at 40°C, pass through a 100-mesh sieve after crushing, and store in the dark for later use;

[0068] 2) Mix the treated mango leaves with 80% ethanol solution at a ratio of 1:8g / mL, heat to 60°C with microwave, and perform ultrasonic extraction 5 times respectively, the extraction power is 250w, and the extraction time is 25min;

[0069] 3) Combine the extracts, centrifuge the extracts at 4°C and 4000r / min for 20min, and take the supernatant;

[0070] 4) The supernatant was rotary evaporated at 50°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0071] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 10BV, and after the purification was completed, it was eluted wit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting high-purity mangiferin from mango leaves. The extraction method of the present invention comprises the steps of mango leaf drying and pulverization, microwave heating, ultrasonic extraction, centrifugation, rotary evaporation, macroporous adsorption resin purification, concentration, and freeze-drying, and has the characteristics of simple operation, low cost, and high extraction rate. There are no special requirements for equipment. The extraction solvent adopted in the invention has the characteristics of green and environmental protection, and will not have adverse effects on the environment and human health. The mangiferin extracted by the invention has high purity, has huge industrial value and good application prospect. The invention can not only make full use of mango leaf resources, provide a new idea for the comprehensive utilization of mango leaf resources, but also produce mangiferin products with high added value, increase the income of fruit farmers, and have huge industrial value and good application prospects.

Description

technical field [0001] The invention relates to the field of extracting plant functional components, in particular to a method for extracting high-purity mangiferin from mango leaves. Background technique [0002] Mango leaves are the leaves of Anacardiaceae mango (Mangifera indica L.), mainly distributed in tropical and subtropical regions such as Guangdong, Guangxi, Yunnan, Hainan, Taiwan, etc., and have the advantages of wide distribution, low price, and easy availability of raw materials. Mango leaves contain ascorbic acid, tannins, mangiferin, flavonoids and other substances. Mango leaf extracts can inhibit the growth and reproduction of pyogenic coccus and Escherichia coli. Sores and other effects. [0003] Mangiferin, also known as Chinonin or Mangiferin, is a carboketone glycoside of tetrahydroxypyrrole, which belongs to diphenone flavonoids. Mangiferin is the main active ingredient in mango leaves. Studies have shown that mangiferin has significant anti-inflammat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 刘家波李震宇朱华兴
Owner 湖北博瑞生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com