Reagent card box for detecting novel coronavirus (SARS-CoV-2) antigen and preparation method and application of reagent card box

Abstract

The invention discloses a reagent card box for detecting a novel coronavirus SARS-CoV-2 antigen and a preparation method of the reagent card box, and belongs to the field of novel coronavirus detection. The reagent card box comprises a reagent card box body, a detection strip arranged in the reagent card box body, a diluent and an operation instruction.The novel coronavirus can be rapidly detected; rapid and large-scale batch operation can be realized, the exposure risk in the specimen collection process of medical personnel can be avoided, the antibody detection is safer, and technical support is provided for epidemic situation prevention and control.

Description

technical field [0001] The invention relates to the field of novel coronavirus detection, in particular to a reagent cartridge for detecting novel coronavirus (SARS-CoV-2) antigen and its preparation method and application. Background technique [0002] New coronary pneumonia is caused by an enveloped single-stranded positive-sense RNA novel coronavirus (named SARS-CoV-2 by the International Committee on Taxonomy of Viruses, aliased as 2019-nCoV). Under the electron microscope, it can be observed that there are obvious rod-shaped particle protrusions on the outer membrane, which look like a crown, hence the name coronavirus. 2019-nCoV is the seventh type of human coronavirus, which belongs to betacoronavirus, has an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. Known coronavirus genome structures such as figure 1 As shown, the 5' end is the open reading frame (open reading frame, ORF) 1a and 1b, accounting for about 2 / 3 of t...

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Problems solved by technology

[0004] Another clinical data shows that some infected patients cannot detect viral nucleic acid, which is inconsistent with the clinical manifestations. This kind of "false negative" cannot be completely avoided. False negative means missed detection, which will not only lead to inability to quickly diagnose suspected patients in clinical practice , and will make those who missed the test a potential source of virus infection

The enzyme-linked immunosorbent assay (ELISA), the most widely used serological detection method, has accurate results, but it is not easy to operate and requires multiple incubations and washings. The operation is cumbersome and time-consuming, and it takes at least 1.5 hours to detect the results.

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