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Preparation method of fish scale collagen and application of fish scale collagen as antioxidant and enzyme inhibitor

A technology of fish scale collagen and compound protease, which is applied in food ingredients as antioxidants, preparation methods and applications of peptides, can solve the problems of poor flavor and poor appearance of fish scale collagen, achieve high crowd acceptance, and avoid adverse effects , The effect of shortening the processing time

Pending Publication Date: 2021-10-01
湖北瑞邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a method for preparing fish scale collagen, which solves the problems of poor appearance and bad flavor of fish scale collagen in the prior art

Method used

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  • Preparation method of fish scale collagen and application of fish scale collagen as antioxidant and enzyme inhibitor
  • Preparation method of fish scale collagen and application of fish scale collagen as antioxidant and enzyme inhibitor
  • Preparation method of fish scale collagen and application of fish scale collagen as antioxidant and enzyme inhibitor

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Embodiment 1

[0023] The present embodiment provides a kind of preparation method of fish scale collagen, it comprises the steps:

[0024] Take 5kg of fresh fish scales as raw material, add 7.5kg of water and 0.25kg of citric acid, then cook at 65°C for 4 hours to make it into a paste, then cool down to below 40°C to obtain a paste, add to the paste 0.0065kg neutral protease and 0.0004kg papain, and adjust the pH of the mixture to 7.0 for hydrolysis, keep the temperature at 40-45°C during the hydrolysis process, adjust the pH to 6.5 after hydrolysis for 3 hours, and then filter to obtain the primary filtrate. Use a filter membrane with a filter hole of 3000D to perform membrane filtration to obtain a dilute liquid with a solid content of 8.1%, then adjust the pH of the dilute liquid to 4.5 and then pass through a carbon fiber column for decolorization, and then adjust the pH of the resultant after decolorization by the carbon fiber column To 6.5 to get the decolorized material liquid, the g...

Embodiment 2

[0026] The present embodiment provides a kind of preparation method of fish scale collagen, it comprises the steps:

[0027] Take 5kg of fresh fish scales as raw material, add 8.75kg of water and 0.35kg of citric acid, then cook at 70°C for 5 hours to make it into a paste, then cool down to below 40°C to obtain a paste, add to the paste 0.0075kg neutral protease and 0.0005kg papain, and adjust the pH of the mixture to 6.5 for hydrolysis. During the hydrolysis process, keep the temperature at 40-45°C. After hydrolysis for 4 hours, adjust the pH to 6.2 and then filter to obtain the primary filtrate. The obtained primary filtrate continues Use a filter membrane with a filter hole of 6000D to perform membrane filtration to obtain a dilute solution with a solid content of 10.2%, then adjust the pH of the dilute solution to 4.2, and then pass through a carbon fiber column for decolorization, and then adjust the pH of the resultant after decolorization by the carbon fiber column To 6...

Embodiment 3

[0029] The present embodiment provides a kind of preparation method of fish scale collagen, it comprises the steps:

[0030] Take 5kg of fresh fish scales as raw material, add 10kg of water and 0.45kg of citric acid, then cook at 75°C for 5.5h to make it into a paste, then cool down to below 40°C to obtain a paste, add to the paste 0.0085kg neutral protease and 0.0006kg papain, and adjust the pH of the mixture to 6.0 for hydrolysis, keep the temperature at 40-45°C during the hydrolysis process, adjust the pH to 6.0 after 4.5 hours of hydrolysis and then filter to obtain the primary filtrate. Continue to carry out membrane filtration with a filter membrane with a filter hole of 10000D to obtain a thinner solution with a solid content of 11.9%, then adjust the pH of the thinner solution to 4.0 and pass through a carbon fiber column for decolorization, and adjust the resulting product after decolorization by the carbon fiber column pH to 6.0 to obtain the decolorized material liq...

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Abstract

The invention provides a preparation method of fish scale collagen. The preparation method comprises the following steps: boiling fish scales to a pasty state, then adding compound protease for hydrolysis, keeping a temperature at 40-45 DEG C in a hydrolysis process, keeping a pH value at 6.0-7.0 in a hydrolysis process, adjusting the pH value to 6.0-6.5 after hydrolysis, carrying out primary filtration, collecting a filtrate, carrying out membrane filtration on the filtrate to obtain thinner liquid, subjecting the obtained thinner liquid to decoloration treatment while the pH value is kept at 4.0-4.5 in a decoloration process, adjusting the pH value to 6.0-6.5 after decoloration to obtain decolored material liquid, concentrating the decolored material liquid to obtain a concentrated liquid with a solid content of 21-28%, and then conducting spray drying to obtain the powdery fish scale collagen. The fish scale collagen obtained by the invention can also be used as an antioxidant and a carbohydrase inhibitor for practical application. The method provided by the invention is efficient in processing, and meanwhile, the appearance and smell characteristics of the fish scale collagen are improved.

Description

technical field [0001] The invention relates to the technical field of comprehensive utilization of fish processing waste, in particular to a method for preparing fish scale collagen and its application as an antioxidant and carbohydrase inhibitor. Background technique [0002] Fish scales are the inedible parts of fish that need to be removed during processing. The removed fish scales generally have no other use and can only be disposed of as kitchen waste. Caused a certain waste of resources. [0003] Chinese invention CN106119330A discloses a preparation method of fish scale collagen hydrolyzed peptide, said preparation method includes steps: raw material pretreatment, preparation of fish scale powder, enzymatic extraction of fish scale collagen, enzymatic hydrolysis to prepare fish scale collagen hydrolyzed peptide; the invention The method of enzymatic preparation of fish scale collagen hydrolyzed peptide was screened and optimized, and the yield of fish scale collage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/34C07K1/14A23L33/17
CPCC07K14/78C12P21/06A23L33/17A23V2002/00A23V2200/02A23V2200/30
Inventor 靳祯亮王希搏王彩霞伍梦婷吴海王微尹晓清
Owner 湖北瑞邦生物科技有限公司
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