Malignant pleural effusion detection method based on multi-dimensional purification and enrichment technology

Abstract

The invention discloses a malignant pleural effusion detection method based on a multi-dimensional purification and enrichment technology. The malignant pleural effusion detection method comprises the following steps that: abdominal tumor pleural effusion of a patient is acquired; a pleural effusion drainage device is used for draining the pleural effusion of the patient through a puncture drainage method, and then the pleural effusion is submitted for inspection; the pleural effusion is analyzed, pathological characteristics are obtained; the to-be-detected pleural effusion is detected by using a flow cytometer, the existence of multinuclear cells is judged, the to-be-detected pleural effusion is centrifuged by using a density gradient centrifugation method to obtain pleural effusion cast-off cells, the pathological type of a tumor is judged on the basis of cytology immunohistochemistry, finally DNA is extracted from the pleural effusion cast-off cells, and molecular gene detection is carried out; a targeted drug and clinical treatment are determined; and sensitive drugs and targeted drugs of tumor cells are determined according to the obtained pathological type and gene mutation data of the tumor cells. The malignant pleural effusion detection method based on the multi-dimensional purification and enrichment technology has the advantages of low cost and easiness in operation, and just cause little harm to patients.

Description

technical field [0001] The invention relates to the technical field of tumor diagnosis and treatment, in particular to a detection method for malignant pleural effusion based on multi-dimensional purification and enrichment technology. Background technique [0002] The current data show that the exfoliated cytological examination of pleural effusion is usually obtained by simple centrifugation to obtain cell pellets and then smeared. Missed diagnosis is also the main reason why the positive rate of tumor cytology diagnosis obtained by cytopathological method of pleural and ascites is less than one-third. In addition, due to the small number of tumor cells in some pleural effusion deposits and their discrete state, it is difficult to judge the pathological type and degree of cell differentiation, and it is difficult to obtain the pathological type. Therefore, improving the purity of tumor cells in pleural effusion will help to increase the positive rate of cytological diagno...

AI Technical Summary

Problems solved by technology

[0002] The current data show that the exfoliated cytological examination of pleural effusion is usually obtained by simple centrifugation to obtain cell pellets and then smeared. Missed diagnosis, which is also the main reason why the positive rate of tumor cytology diagnosis obtained by cytopathological method of pleural and peritoneal fluid is less than one-third

In the past, EGFR mutation detection for non-small cell carcinoma was basically extracted from tissue paraffin specimens. Due to dehydration, repair and other processes during the preparation of paraffin specimens, some DNA will be broken, and the integrity of DNA will be damaged. destroy

Especially for the small number of tumor cells in the paraffin specimens of pleural effusion cells, coupled with paraffin embedding, it is difficult to obtain the complete DNA of tumor cells, which makes it impossible to carry out targeted drugs based on the molecular detection of tumor cells DNA