A Multifunctional Microscopic Imaging Slide Based on Optical Thin Films

Abstract

The invention discloses a multifunctional microscopic imaging slide based on an optical film, comprising: a photonic crystal incident layer, a scattering film layer, a photonic crystal functional layer, and a sample layer; each layer of the invention can be processed layer by layer by means of film layer processing It can also be prepared separately and then combined with refractive index matching oil. For the first time, this imaging slide uses a variety of one-dimensional photonic crystal composite structures specially designed and prepared to convert normal incident light into large numerical aperture outgoing light, total internal reflection light and surface plasmon, which is successfully realized on traditional microscopes. High-contrast darkfield and surface plasmon imaging.

Description

technical field [0001] The invention relates to the field of high-contrast dark-field optical microscopic imaging, in particular to the field of a multifunctional microscopic imaging slide based on an optical film. Background technique [0002] Microscopy is the most direct way for people to understand the microscopic world. Optical microscopy directly presents the images of the microscopic world in front of our eyes. It is the most intuitive and commonly used microscopic technique among all microscopic techniques. Dark field microscopy reduces the collection of illumination light and enhances the collection of scattered light after illumination in the process of optical microscopy to improve the signal-to-noise ratio of the imaging system. Depending on the numerical aperture of the illumination light, darkfield microscopes can provide illumination methods such as transmission and total internal reflection. The above-mentioned main microscopic techniques have certain limita...

AI Technical Summary

Problems solved by technology

[0006] The technical problem of the present invention is to overcome the disadvantages of traditional dark-field microscopes, such as complex optical structure, high use cost and poor convenience, and provide a multi-functional microscopic imaging slide based on optical film. Structure, using the vertically incident illumination light of the traditional microscope system, realizes multiple functions including large numerical aperture transmission, total reflection dark field and surface plasmon illumination for one or more working wavelengths at the same time, reducing the cost of the dark field microscope system cost of use and improve ease of use

Images