Marine bioactive peptide capable of effectively relieving skin sunburn, and preparation method and application of marine bioactive peptide

A technology for marine organisms and active peptides, applied in the field of biomedicine, can solve the problems of difficult large-scale industrial production and application, low yield of active peptides, complicated preparation methods, etc., and achieves short production cycle, simple preparation process and good physiological effect. Effect

Active Publication Date: 2021-10-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the preparation methods involved are relatively complicated, coupled with the limitation of the sample processing capacity of high-precision separation techniques such as high-performance liquid chromatography, the yield of the final active peptide is low and the preparation cost is high, making it difficult to carry out large-scale industrial production and application.

Method used

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  • Marine bioactive peptide capable of effectively relieving skin sunburn, and preparation method and application of marine bioactive peptide
  • Marine bioactive peptide capable of effectively relieving skin sunburn, and preparation method and application of marine bioactive peptide
  • Marine bioactive peptide capable of effectively relieving skin sunburn, and preparation method and application of marine bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Take fresh and live clam scallops that meet the standards for the use of relevant seafood in my country, remove the shell, homogenize the soft body, and add 3 times the volume of PBS buffer (0.01M, pH7.5), neutral protease (3000U / g shellfish) , temperature 45°C, enzymatic hydrolysis for 4h. The enzymolysis solution was boiled for 10 minutes and centrifuged at 7000×g for 12 minutes. The supernatant was ultrafiltered sequentially through ultrafiltration membranes with a molecular weight cut-off of 10 kDa and 3 kDa. Among them, the 3kDa-10kDa active peptide component is recorded as N1, and the active peptide component less than 3kDa is recorded as N2. Concentrate the N1 and N2 components to a concentration of about 100mg / mL, and then separate them with Sephadex G-15 (100×4.0cm): the loading volume is 15mL, the mobile phase is distilled water, the flow rate is 10mL / min, and the detection wavelength 220nm, 280nm.

[0069] The N1 (3kDa-10kDa) fraction was separated by Sepha...

Embodiment 2

[0075] Take fresh and live clam scallops that meet the standards for the use of relevant seafood in my country, remove the shell, homogenize the soft body, add 2.5 times the volume of distilled water, adjust the pH value to 7.0, add neutral protease (2000U / g shellfish), and the temperature is 50°C , enzymatic hydrolysis for 5h. The enzymolysis solution was boiled for 10 minutes and centrifuged at 6000×g for 15 minutes. The supernatant was ultrafiltered sequentially through ultrafiltration membranes with a molecular weight cut-off of 10 kDa and 3 kDa. Among them, the 3kDa-10kDa active peptide component is recorded as N1, and the active peptide component less than 3kDa is recorded as N2. Concentrate the N1 and N2 components to a concentration of about 200mg / mL, and then separate them with Sephadex G-15 (100×4.0cm): the loading volume is 7mL, the mobile phase is distilled water, the flow rate is 10mL / min, and the detection wavelength 220nm, 280nm.

[0076] Separate the N1 (3kDa...

Embodiment 3

[0081]Take the fresh and live clam shells that meet the standards for the use of relevant seafood in my country, remove the shells, take the soft part of the homogenate, add 4 times the volume of deionized water, adjust the pH to 6.5, add neutral protease (4000U / g shellfish), and in the Temperature 37 ℃, enzymatic hydrolysis 6h. The enzymolysis solution was boiled for 10 minutes and centrifuged at 9000×g for 10 minutes. The supernatant was ultrafiltered through an ultrafiltration membrane with a molecular weight cut-off of 10 kDa, and the filtrate (active peptide fraction less than 10 kDa) was recorded as N. Concentrate the N component to a concentration of about 100mg / mL, and then separate it with Sephadex G-15 (100×4.0cm): the loading volume is 16mL, the mobile phase is distilled water, the flow rate is 10mL / min, and the detection wavelength is 220nm and 280nm .

[0082] After the N (less than 10kDa) fraction is separated by Sephadex G-15, the first to sixth elution peak fr...

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Abstract

The invention discloses a marine bioactive peptide capable of effectively relieving skin sunburn, and a preparation method and application of the marine bioactive peptide. The marine bioactive peptide is small in molecular weight, good in skin compatibility and capable of remarkably reducing apoptosis and necrosis of cells irradiated by ultraviolet rays and reducing true dermatitis exudation and elastic fibre degradation. The preparation method of the marine bioactive peptide comprises the following steps of: taking a soft part of a fresh marine shellfish, carrying out protease enzymolysis, ultrafiltration and sephadex separation, discarding the last elution single peak, respectively collecting or mixing other elution components, and performing freeze-drying to obtain the marine bioactive peptide. The method is simple, low in cost, green, environment-friendly, short in production cycle and suitable for industrial production; the prepared active peptide has the effects of preventing and repairing skin sunburn and can be applied to cosmetics and biomedical materials; the cosmetic dosage forms include cream, emulsion, gel, jelly, water aqua, mist spray and the like; and dosage forms of the biomedical material comprise lotion, solution, dressing (thin film, hydrocolloid, hydrogel, sponge and spray), ointment, injection and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a marine bioactive peptide capable of effectively alleviating skin sunburn, a preparation method and application thereof. Background technique [0002] With the destruction of the ozone layer, the skin is exposed to excessive ultraviolet rays for a long time, leading to an increase in the incidence of actinic skin diseases, basal cell carcinoma, and squamous cell carcinoma. The formation and occurrence of skin cancer induced by ultraviolet rays is a complex and continuous biological behavior, in which ultraviolet rays (UVB) with a wavelength of 280-320nm are located near the absorption peaks of DNA and proteins, can cause DNA and protein damage, and are the most carcinogenic or mutation. The DNA damage of epidermal keratinocytes by ultraviolet rays is the basis for inducing skin cancer. If the damaged DNA undergoes unrestricted repairs, it will cause mutations in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A61K8/64A61Q19/00A61Q17/04A61K38/01A61P17/00A61P17/16
CPCC12P21/06C07K1/34A61K8/64A61Q19/004A61Q17/04A61P17/00A61P17/16A61K38/00Y02A50/30
Inventor 陈华孙恢礼潘剑宇蔡冰娜万鹏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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