The invention provides a fructus cannabis protein biscuit and a preparation method thereof. The fructus cannabis protein biscuit comprises the following components in parts by weight: 100-150 parts of low-gluten flour, 10-20 parts of fructus cannabis protein powder, 10-30 parts of green banana whole meal, 2-8 parts of ultrafine powder of grape seed, 10-12 parts of custard flour, 40-60 parts of butter, 10-15 parts of white granulated sugar, 15-25 parts of whole egg liquid, 0.3-0.8 part of salt, 0.3-0.6 part of sodium bicarbonate, 1-2 parts of baking powder, 5-15 parts of whole milk powder and 80-120 parts of water. The fructus cannabis protein biscuit has the beneficial effects that firstly, the fructus cannabis protein component is beneficial for human health, and is easy to digest and absorb after being separated and extracted; secondly, the compound green banana whole meal has the physiological effects of enhancing the prevention and cure of bowel diseases, reducing the blood sugar and the like; the grape seed has the physiological effects of resisting oxidation, increasing the immunity and the like; thirdly, the biscuit disclosed by the invention has the characteristics of high protein, high cellulose and low fat, and the physiological effects of the fructus cannabis protein can be well maintained; fourthly, the biscuit also has the functions of promoting gastrointestinal motility, promoting digestion, adjusting the gastroenteric environment, resisting oxidation, reducing blood fat, reducing blood pressure, reducing cholesterol, resisting fatigue and the like.