Marine biological medical material for repairing skin wounds as well as preparation method and application of marine biological medical material

A technology for skin wounds and marine organisms, applied in medical science, bandages, absorbent pads, etc., can solve problems such as poor wound healing quality, achieve large encapsulation rate, excellent water absorption and water retention, uniform and suitable microsphere size Effect

Active Publication Date: 2022-04-12
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that large-area full-thickness skin defects easily lead to severe infection and poor wound healing quality, the present invention prepares a scaffold loaded with PDGF-BB@calcium alginate microspheres / collagen / chitosan

Method used

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  • Marine biological medical material for repairing skin wounds as well as preparation method and application of marine biological medical material
  • Marine biological medical material for repairing skin wounds as well as preparation method and application of marine biological medical material
  • Marine biological medical material for repairing skin wounds as well as preparation method and application of marine biological medical material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Preparation of PDGF-BB@calcium alginate microspheres: Sodium alginate (purchased from Shanghai McLean Biochemical Technology Co., Ltd., purity 98%) was dissolved in distilled water to prepare a concentration of 2% w / v alginate Sodium solution, by mass ratio 1:1.0×10 -5 Add PDGF-BB, stir well to make an aqueous phase; measure a certain volume of olive oil (purchased from Shanghai McLean Biochemical Technology Co., Ltd.), add Tween 80 (purchased from Shanghai McLean Biochemical Technology Co., Ltd.) at a ratio of 1% v / v purchased from the company), stirred at 600rpm for 2h, and was prepared as an organic phase; 100mL of the organic phase was measured, and 20mL of the aqueous phase was added under the stirring condition of 650rpm, stirred at 750rpm for 1h, and 40mL of calcium chloride (from Shanghai Mike Lin Biochemical Technology Co., Ltd.) solution, stirred at 500 rpm for 1 h, then added 10 mL of isopropanone (Tianjin Fuyu Chemical Reagent Factory, AR grade), stirred...

Embodiment 2

[0086] (1) Preparation of PDGF-BB@calcium alginate microspheres: Sodium alginate was dissolved in distilled water to prepare a sodium alginate solution with a concentration of 2% w / v, and the mass ratio was 1:0.2×10 -5 Add PDGF-BB, stir well to make the water phase; measure a certain volume of olive oil, add Tween 80 at a ratio of 1% v / v, stir at 750rpm for 1h, and prepare the organic phase; measure 100mL of the organic phase, stir at 700rpm Add 20mL of water phase, stir at 750rpm for 1h, add 40mL of calcium chloride solution with a concentration of 1% w / v, stir at 500rpm for 1h, then add 10mL of isopropanone, stir at 400rpm for 30min, centrifuge the mixture at 6000rpm for 10min, and use The PDGF-BB@calcium alginate microspheres were obtained by alternately washing twice with acetone and distilled water, and then freeze-drying.

[0087] (2) Preparation of calcium alginate microspheres: the preparation method is the same as (1), except that PDGF-BB is not added.

[0088] (3) P...

Embodiment 3

[0096] (1) Preparation of PDGF-BB@calcium alginate microspheres: Sodium alginate was dissolved in distilled water to prepare a sodium alginate solution with a concentration of 2% w / v, and the mass ratio was 1:0.1×10 -5 Add PDGF-BB, stir well to make the water phase; measure a certain volume of olive oil, add Tween 80 at a ratio of 1% v / v, stir at 700rpm for 1.5h, and prepare the organic phase; measure 100mL of the organic phase, stir at 700rpm Add 20 mL of water phase, stir at 750 rpm for 1 h, add 40 mL of calcium chloride solution with a concentration of 1% w / v, stir at 500 rpm for 1 h, then add 10 mL of isopropanone, stir at 400 rpm for 30 min, and centrifuge the mixture at 6000 rpm for 8 min. The PDGF-BB@calcium alginate microspheres were prepared by washing with isopropanone and distilled water alternately for 3 times, and then freeze-dried.

[0097] (2) Preparation of calcium alginate microspheres: the preparation method is the same as (1), except that PDGF-BB is not adde...

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Abstract

The invention discloses a marine biological medical material for repairing skin wounds as well as a preparation method and application of the marine biological medical material. From a plurality of marine bioactive substances, preferably, marine fish skin collagen, carboxymethyl chitosan and alginate which are good in biocompatibility and easy to process and degrade are scientifically combined with platelet-derived growth factors PDGF-BB, and optimization is carried out, so that the marine collagen-based hydrogel is prepared. The material has the advantages of being high in porosity, high in swelling ratio, good in mechanical property, capable of controlling slow release of PDGF-BB, free of cytotoxicity and the like, cell adhesion, proliferation, migration and vascularization can be remarkably promoted, inflammatory response is effectively relieved, and scar hyperplasia is reduced. The biomedical material is wide in application range and convenient to use, can prevent bacterial infection without frequent replacement, and has remarkable effects of promoting regeneration and repair of burns, scalds, cutting wounds, traffic wounds, surgical wounds, war wounds, firearm wounds combined with seawater soaking wounds, diabetic ulcers, inflammatory ulcers, pressure ulcers and chronic refractory wounds.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a marine biomedical material for skin wound repair and its preparation method and application. Background technique [0002] Although most skin defects can be healed quickly and effectively within 1 or 2 weeks, extensive full-thickness wounds are often difficult to repair, which will seriously affect health and even directly threaten life. Skin defects and abnormalities such as trauma, ulcers, inflammation, burns, scalds, surgery and congenital deformities will not only bring physical pain to patients, but also cause psychological trauma. Therefore, there is an urgent need to develop a biomedical material with a significant healing effect to help patients improve their quality of life. [0003] The marine habitat is unique and rich in biological resources, and it is a treasure house of resources for finding precious medicines. Marine-derived polymer materials such as inorgan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/44A61L15/42A61L15/46A61L26/00
Inventor 陈华孙恢礼潘剑宇万鹏蔡冰娜陈得科
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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