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A marine biomedical material for skin wound repair and its preparation method and application

A technology for skin wounds and marine organisms, applied in medical science, bandages, absorbent pads, etc., can solve problems such as poor wound healing quality, and achieve the effects of large encapsulation rate, good permeability, and appropriate mechanical strength

Active Publication Date: 2022-07-26
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems that large-area full-thickness skin defects easily lead to severe infection and poor wound healing quality, the present invention prepares a scaffold loaded with PDGF-BB@calcium alginate microspheres / collagen / chitosan

Method used

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  • A marine biomedical material for skin wound repair and its preparation method and application
  • A marine biomedical material for skin wound repair and its preparation method and application
  • A marine biomedical material for skin wound repair and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0075] (1) Preparation of PDGF-BB@calcium alginate microspheres: Dissolve sodium alginate (purchased from Shanghai McLean Biochemical Technology Co., Ltd., purity 98%) in distilled water to prepare a concentration of 2% w / v alginic acid Sodium solution, by mass ratio 1:1.0×10 -5 Add PDGF-BB, stir well to make water phase; measure a certain volume of olive oil (purchased from Shanghai McLean Biochemical Technology Co., Ltd.), add Tween 80 (from Shanghai McLean Biochemical Technology Co., Ltd.) at a ratio of 1% v / v purchased from the company), stirred at 600 rpm for 2 h, and prepared into an organic phase; measure 100 mL of organic phase, add 20 mL of aqueous phase under stirring at 650 rpm, stir at 750 rpm for 1 h, and add 40 mL of calcium chloride with a concentration of 1% w / v (from Shanghai Mike Lin Biochemical Technology Co., Ltd.) solution, stirred at 500 rpm for 1 h, then added 10 mL of isoacetone (Tianjin Fuyu Chemical Reagent Factory, AR grade), stirred at 400 rpm for 3...

Embodiment 2

[0086] (1) Preparation of PDGF-BB@calcium alginate microspheres: Dissolve sodium alginate in distilled water to prepare a sodium alginate solution with a concentration of 2% w / v, in a mass ratio of 1:0.2×10 -5 Add PDGF-BB, stir well to make water phase; measure a certain volume of olive oil, add Tween 80 at a ratio of 1% v / v, and stir at 750 rpm for 1 h to prepare an organic phase; measure 100 mL of organic phase, stir at 700 rpm Add 20 mL of aqueous phase, stir at 750 rpm for 1 h, add 40 mL of calcium chloride solution with a concentration of 1% w / v, stir at 500 rpm for 1 h, then add 10 mL of isoacetone, stir at 400 rpm for 30 min, the mixture is centrifuged at 6000 rpm for 10 min, and the obtained precipitates were separated by isoacetone. Acetone and distilled water were alternately washed twice, and freeze-dried to obtain PDGF-BB@calcium alginate microspheres.

[0087] (2) Preparation of calcium alginate microspheres: the preparation method is the same as (1), except that ...

Embodiment 3

[0096] (1) Preparation of PDGF-BB@calcium alginate microspheres: Dissolve sodium alginate in distilled water to prepare a sodium alginate solution with a concentration of 2% w / v, in a mass ratio of 1:0.1×10 -5 Add PDGF-BB, stir well to make water phase; measure a certain volume of olive oil, add Tween 80 at a ratio of 1% v / v, and stir at 700 rpm for 1.5 h to prepare an organic phase; measure 100 mL of organic phase, stir at 700 rpm Add 20 mL of aqueous phase, stir at 750 rpm for 1 h, add 40 mL of calcium chloride solution with a concentration of 1% w / v, stir at 500 rpm for 1 h, then add 10 mL of isoacetone, stir at 400 rpm for 30 min, and centrifuge the mixture at 6000 rpm for 8 min. Isoacetone and distilled water were alternately washed three times, and freeze-dried to obtain PDGF-BB@calcium alginate microspheres.

[0097] (2) Preparation of calcium alginate microspheres: the preparation method is the same as (1), except that PDGF-BB is not added.

[0098] (3) Preparation of...

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Abstract

The invention discloses a marine biomedical material for repairing skin wounds and a preparation method and application thereof. From many marine biological actives, the preferred marine fish skin collagen, carboxymethyl chitosan, alginate with good biocompatibility, easy processing and degradation, and the platelet-derived growth factor PDGF-BB are scientifically combined and optimized for preparation made. The material has the advantages of high porosity, high swelling ratio, good mechanical properties, controlled release of PDGF-BB, and no cytotoxicity. It can significantly promote cell adhesion, proliferation, migration and angiogenesis, effectively reduce inflammation and reduce scarring. . The biomedical material has a wide range of applications, is easy to use, can prevent bacterial infection without frequent replacement, and is effective in promoting burns, scalds, cuts, traffic injuries, surgical injuries, war injuries, firearm injuries combined with seawater immersion injuries, diabetic ulcers, inflammation The regeneration and repair effect of chronic ulcers, pressure ulcers and chronic refractory wounds is remarkable.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a marine biomedical material for skin wound repair and a preparation method and application thereof. Background technique [0002] While most skin defects heal quickly and effectively within 1 or 2 weeks, extensive full-thickness wounds are often difficult to repair, causing serious health consequences and even direct life-threatening consequences. Skin defects and abnormalities such as trauma, ulcers, inflammation, burns, scalds, operations and congenital deformities will not only bring physical pain to patients, but also cause psychological trauma. Therefore, there is an urgent need to develop a biomedical material with a significant healing-promoting effect to help patients improve their quality of life. [0003] The marine habitat is unique and contains abundant biological resources, which is a treasure house of resources to find precious medicines. Marine-derived macrom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/44A61L15/42A61L15/46A61L26/00
Inventor 陈华孙恢礼潘剑宇万鹏蔡冰娜陈得科
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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