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Method for promoting accumulation of astaxanthin in haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield and efficiency, high production cost, and achieve short induction time and low cost. , the effect of increasing the content

Active Publication Date: 2021-10-19
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still problems such as high production cost, low output and low efficiency.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] At a temperature of 25°C and a light intensity of 20μmol / m 2 / s and light-dark ratio of 12h / 12h, the cells of Haematococcus pluvialis were cultured with MCM medium (see Table 1) to reach the plateau stage. Then the Haematococcus pluvialis cells were centrifuged and placed into new normal MCM medium (see Table 1) to form a Haematococcus pluvialis algae liquid, at which time the cell density was 73,000 cells / mL. Then add sodium fumarate until the content of sodium fumarate in every liter of Haematococcus pluvialis liquid is 10mM. 2 / s and the light-dark ratio of 12h / 12h induced the accumulation of astaxanthin.

[0022] When cultured to the 12th day, the content of astaxanthin in the cells of Haematococcus pluvialls added with sodium fumarate was 31.77pg / cell, which was 29.1% higher than that of the control group without sodium fumarate, indicating that sodium fumarate can significantly promote normal Accumulation of astaxanthin in Haematococcus pluvialis under culture c...

Embodiment 2

[0024] At a temperature of 25°C and a light intensity of 20μmol / m 2 / s and light-dark ratio of 12h / 12h, the cells of Haematococcus pluvialis were cultured with MCM medium (see Table 1) to reach the plateau stage. Then the Haematococcus pluvialis cells were centrifuged and placed into new normal MCM medium (see Table 1) to form a Haematococcus pluvialis algae liquid, at which time the cell density was 73,000 cells / mL. Then add sodium fumarate until the content of sodium fumarate in every liter of Haematococcus pluvialis liquid is 20mM. 2 / s and the light-dark ratio of 12h / 12h induced the accumulation of astaxanthin.

[0025] When cultured to the 12th day, the content of astaxanthin in the algae cells added with sodium fumarate was 62.34pg / cell, which was 153.3% higher than that of the control group without sodium fumarate, indicating that sodium fumarate can significantly promote the growth of rain under normal culture conditions. Accumulation of astaxanthin in Haematococcus ...

Embodiment 3

[0027] At a temperature of 25°C and a light intensity of 20μmol / m 2 / s and light-dark ratio of 12h / 12h, the cells of Haematococcus pluvialis were cultured with MCM medium (see Table 1) to reach the plateau stage. Then the Haematococcus pluvialis cells were centrifuged and placed into a new nitrogen-deficient MCM medium (see Table 2) to form a Haematococcus pluvialis algae liquid, at which time the cell density was 75,000 cells / mL. Then add sodium fumarate until the content of sodium fumarate in every liter of Haematococcus pluvialis liquid is 1mM. 2 / s and the light-dark ratio of 12h / 12h induced the accumulation of astaxanthin.

[0028] When cultured to the 12th day, the content of astaxanthin in algae cells added with sodium fumarate was 34.43pg / cell, which was 56.8% higher than that of the control group without sodium fumarate, indicating that sodium fumarate can significantly promote the growth of rain under nitrogen deficiency conditions. Accumulation of astaxanthin in H...

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Abstract

The invention discloses a method for promoting accumulation of astaxanthin in haematococcus pluvialis. The method comprises the following steps: culturing haematococcus pluvialis cells to reach a platform phase, centrifuging the haematococcus pluvialis cells, inoculating the centrifuged haematococcus pluvialis cells into a new culture medium to form a haematococcus pluvialis solution, and adding sodium fumarate into the haematococcus pluvialis solution during light induction to promote accumulation of astaxanthin. According to the method disclosed by the invention, astaxanthin accumulation in haematococcus pluvialis cells can be remarkably accelerated, the content of astaxanthin in haematococcus pluvialis is increased, the yield of astaxanthin produced by large-scale culture of haematococcus pluvialis is increased, and comprehensive economic benefits are improved.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a method for promoting astaxanthin accumulation in Haematococcus pluvialis. Background technique [0002] Astaxanthin, a red fat-soluble carotenoid, has broad market application prospects in the fields of aquaculture, cosmetics, food and medical health products because of its strong coloring power and antioxidant activity. Astaxanthin has three optical isomers: left-handed 3S, 3′S, right-handed 3R, 3′R and racemic 3S, 3′R, and the left-handed astaxanthin is more active than the other two configurations. Astaxanthin has stronger antioxidant activity and biological functions. Haematococcus pluvialis is the organism with the highest known astaxanthin content in nature, accounting for about 1-5% of the dry cell weight, and the astaxanthin produced is all L-type, so it is regarded as a high-quality natural astaxanthin source. As the market demand for natural astaxanthin incr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/12C12N1/38C12R1/89
CPCC12P23/00C12N1/12C12N1/38Y02A40/81
Inventor 刘建国于文杰张立涛
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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