Biomarker and method for DNA sample tracking detection, and application of biomarker

Abstract

The invention discloses a biomarker and a method for DNA sample tracking detection, and application of the biomarker. A specific multiple PCR primer sequence is designed according to screened 26 SNP loci, a polluted sample to be detected can be tracked and detected, and the biomarker is particularly suitable for verification of sample adding sequence confusion of samples. By adopting the method provided by the invention to track and detect the DNA sample suspected to be polluted, the period of experimental cause searching is greatly shortened, the process monitoring can be performed on the clinical sample, the NGS sequencing platform can be fully utilized, other equipment does not need to be added, and the strict requirement of the clinical sample on the detection period can be met.

Description

technical field [0001] The invention relates to the field of gene detection and tracking, in particular to a biomarker, method and application for DNA sample tracking and detection. Background technique [0002] At present, high-throughput sequencing technology (Next Generation Sequencing, NGS) has gradually emerged in clinical diagnostic applications, and is widely used in many fields such as early cancer screening, cancer companion diagnosis, auxiliary diagnosis of genetic diseases, non-invasive prenatal diagnosis, and pathogenic microorganism detection. , increasingly exerting a powerful detection ability. However, while the technology is developing rapidly, the corresponding laboratory internal quality control has developed relatively slowly. NGS technology involves nucleic acid extraction, library construction, and some even need to involve complex probe hybridization and capture links. Before the final sequencing on the machine, it needs to go through more than 20 ste...

AI Technical Summary

Problems solved by technology

However, due to the different regions covered by different whole exome sequencing panels, other brands of panel (panel refers to a group or a set of detection) probes cannot cover the above 340 SNPs, and what detection method is used to detect these 340 sites It is also a problem to be solved for comparative verification

In addition, the use of multiple SNP sites will increase the corresponding data, increasing the complexity of data processing and detection costs

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