Shiitake mushroom monokaryon culture medium as well as preparation method and application thereof

A monokaryon and culture medium technology, applied in its preparation, in the field of mushroom monokaryon medium, can solve the problems of difficult breeding and protoplast preparation, slow growth of monokaryon strains, slow growth of hyphae, etc., and achieve a good market The effect of application value, shortening the breeding cycle, and dense mycelium growth

Pending Publication Date: 2021-10-22
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, monokaryotic strains have problems such as slow growth and easy aging of mycelium, and there are certain difficulties in subsequent breeding and protoplast preparation.
When the traditional PDA medium is used to culture monokaryotic hyphae of Lentinus edodes, the mycelium grows slowly and is easy to age

Method used

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  • Shiitake mushroom monokaryon culture medium as well as preparation method and application thereof
  • Shiitake mushroom monokaryon culture medium as well as preparation method and application thereof
  • Shiitake mushroom monokaryon culture medium as well as preparation method and application thereof

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preparation example Construction

[0034] Preparation of S2, PDA medium body

[0035] The preparation process of the PDA medium body comprises: boiling potatoes in water and filtering to obtain potato filtrate, and mixing the potato filtrate with glucose and agar. In terms of parts by mass, the raw materials of the PDA medium body include 180-220 parts of potatoes, 15-25 parts of glucose and 15-25 parts of agar, and it is advisable to control potatoes, glucose and agar within the above ranges. Wherein, in the potato cooking step, the quality of potatoes corresponding to every liter of water is controlled to be 190-210g, so as to better control the content of active ingredients in the final medium.

[0036] In order to further promote the growth of mushroom monocytes, the inventors further optimized the ingredients of the PDA medium body. In terms of parts by mass, the raw materials of the PDA medium body include 190-210 parts of potatoes, 18-23 parts of glucose and 18-20 parts of agar.

[0037] In an optional...

Embodiment 1

[0049] The present embodiment provides a kind of preparation method of Lentinus edodes monokaryon culture medium, comprises the steps:

[0050] (1) Take the sawdust, dry it, crush it through a 30-mesh sieve, sterilize it at 121°C for 3 hours, and set it aside;

[0051] (2) Take 200g of potatoes and cut into pieces, put them in 800mL of distilled water and boil until the potato pieces are soft but not rotten, and filter through 8 layers of gauze to obtain the filtrate;

[0052] (3) The potato filtrate is mixed with 20g glucose and 20g agar, and water is added to make up 1L to obtain the PDA medium body;

[0053] (4) Add the wood powder sterilized in step (1) to the body of the PDA medium in step (3), shake it evenly, and then sterilize it at 121°C for 20 minutes. The mass ratio of the body is 1:100 (the amount of wood powder added is roughly 10g);

[0054] (5) The sterilized culture medium was poured into a 90mm×90mm petri dish and left overnight for use.

Embodiment 2

[0056] The present embodiment provides a kind of preparation method of shiitake mushroom monokaryon culture medium, and the difference with embodiment 1 is only: in step (4), the mass ratio of the addition of control sawdust powder and PDA culture medium body is 1.5:100 (wood chips The addition amount of powder is roughly 15g).

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Abstract

The invention discloses a shiitake mushroom monokaryon culture medium as well as a preparation method and application thereof, and relates to the technical field of culture medium preparation. The shiitake mushroom monokaryon culture medium is obtained by mixing a PDA culture medium body and wood chip powder, wherein the mass ratio of the PDA culture medium body to the wood chip powder is 100 : (1-2). According to the present invention, by adding the specific amount of the wood chip powder to the traditional PDA culture medium, the shiitake mushroom monokaryon hypha aging can be effectively retarded, and the hypha growth speed can be improved so as to effectively improve the slow subsequent breeding speed.

Description

technical field [0001] The invention relates to the technical field of medium preparation, in particular to a mushroom monokaryon medium, its preparation method and application. Background technique [0002] Lentinula edodes is one of the most important cultivars of edible fungi in my country. Among the edible fungi, Lentinula edodes has the highest output and the market demand is very large. With the rapid development of the shiitake mushroom industry, consumers have higher and higher requirements for the quality of shiitake mushrooms, and high-quality strains mainly depend on the breeding of shiitake mushrooms, among which monokaryotic strains are important breeding materials for shiitake mushrooms. [0003] However, monokaryotic strains have problems such as slow growth and easy aging of mycelium, and there are certain difficulties in subsequent breeding and protoplast preparation. When the traditional PDA medium is used to culture monokaryotic hyphae of Lentinus edodes,...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 侯娣鲍大鹏杨瑞恒
Owner SHANGHAI ACAD OF AGRI SCI
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