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Bordetella phage culture medium and culture method

A technology of Bordetella and a culture method, applied in the field of Bordetella phage medium and culture, can solve problems such as defects, large differences in phage growth characteristics, difficulty in high-density culture, etc., and achieve high-density culture, effective culture, The effect of high release pot titer

Active Publication Date: 2021-10-22
河南兴华生物技术有限公司
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AI Technical Summary

Problems solved by technology

Bacteriophages belong to viruses, and their culture hosts are bacteria, and the growth characteristics of different phages are quite different, so it is difficult to use the same method for high-density cultivation of different phages
[0005] At present, there are still defects in the culture technology of Bordetella phages, especially Bordetella multocida phages, and it is impossible to achieve high-density cultivation of Bordetella phages

Method used

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  • Bordetella phage culture medium and culture method
  • Bordetella phage culture medium and culture method

Examples

Experimental program
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Embodiment 1

[0024] 1. Seed solution preparation:

[0025] Preparation of Bordetella seed liquid: Streak inoculation of Bordetella strains on TSA (Tryptone Soya Agar) solid plate, culture at 37°C for 24 hours, and then pick a single colony and transfer it into TSB (Tryptone Soya Broth) medium shake flask at 37°C. ℃, 140rpm shaker shaking culture for 16h to obtain pure culture seed bacterial liquid.

[0026] Preparation of Bordetella phage seeds: evenly spread 10 microliters of Bordetella phage liquid on a TSA double-layer plate and incubate at 37°C for 24 hours. After plaques appear on the surface of the plate, scrape off the plaques and wash with sterile TSB. Next, use a 0.22 micron filter membrane to filter and sterilize.

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Abstract

The invention relates to the technical field of bacteriophage culture, in particular to a bordetella bacteriophage culture medium and a culture method. The culture medium provided by the invention comprises water, polypeptone, yeast extract powder, tryptone, sodium chloride and sodium citrate. The culture medium can realize large-scale and high-density culture of the bordetella bacteriophage. Experiments show that compared with a culture medium composed of other components, the culture medium provided by the invention can be used for more effectively culturing the bordetella bacteriophage to obtain higher tank titer.

Description

technical field [0001] The invention relates to the technical field of phage culture, in particular to a Bordetella phage culture medium and a culture method. Background technique [0002] Porcine atrophic rhinitis is a chronic respiratory infection caused by Bordetella bronchiseptica and / or toxigenic Pasteurella multocida infection. When Bordetella bronchiseptica is infected alone, the nasal cavity lesions are mild, but if Bordetella bronchiseptica and toxigenic Pasteurella multocida are mixed or secondary infected, the nasal cavity lesions are severe. The disease can occur throughout the year, with more occurrences in spring and autumn. All pigs can be infected, and pigs aged 2-5 months are more susceptible to infection. It is characterized by turbinate atrophy and chronic rhinitis. The mortality rate of swine atrophic rhinitis is not high, but the growth of sick pigs is slow, the fattening time will be prolonged, the breeding pigs will carry germs, and the breeding value...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K35/76A61P11/02
CPCC12N7/00A61K35/76A61P11/02C12N2795/00051C12N2795/00032Y02A50/30
Inventor 李国强李海超刘美张媛媛孔德江常世恺刘荣飞曹凯典
Owner 河南兴华生物技术有限公司
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