DNA methylation site analysis device and method based on triple helix nucleic acid structure

Abstract

The invention discloses a DNA methylation site analysis device and method based on a triple helix nucleic acid structure, and the device comprises an analyzer body, a workbench, a damping assembly, a station plate, an antiskid assembly, a sample box, a connection assembly, a protection cover, a triple helix nucleic acid structure photoelectrochemical biosensor, a fluorescence excitation lamp and a touch display screen. The workbench is arranged on one side of the top of the analyzer body, the center of the top of the workbench is fixedly connected with the bottom of a damping assembly, and the damping assembly is composed of a damping plate, a single-end stud, a rubber cushion plate, a limiting nut and a buffer spring. The anti-skid structure is additionally arranged, so that the problem that a sample topples and leaks in the moving process of the device is solved, resources are saved, resampling is not needed, the analysis cost of methylation sites is reduced, and the connecting structure is additionally arranged, so that a worker can conveniently disassemble and assemble the protective part, maintenance and replacement are rapid, the labor intensity of the worker is reduced, and the maintenance cost of the device is saved.

Description

technical field [0001] The invention relates to the technical field of analysis devices, in particular to a DNA methylation site analysis device and method based on a triple-helix nucleic acid structure. Background technique [0002] DNA methylation generally refers to the process of selectively adding a methyl group to the cytosine of a genomic CpG dinucleotide to form 5mC under the action of DNA methyltransferase, which can be achieved without changing the DNA sequence Under this condition, it causes changes in chromatin structure, DNA conformation, DNA stability and the way DNA interacts with proteins, thereby controlling gene expression and changing genetic expression. DNA methylation is an important epigenetic mark that plays a major role in biological processes such as regulation of gene expression, maintenance of chromatin structure, gene imprinting, X chromosome inactivation, and embryonic development. [0003] At present, the industry mainly uses devices such as DN...

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Problems solved by technology

[0008] The object of the present invention is to provide a DNA methylation site analysis device and method based on triple-helix nucleic acid structure, so as to solve the problems of sample leakage, high maintenance cost and large vibration amplitude in the above-mentioned background technology

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