Bacillus subtilis microbial agent and preparation method and application thereof

A technology of Bacillus subtilis and bacteria agent, applied in the field of microorganisms, can solve the problems of loss of biodiversity, plant death, increase of water and soil erosion, etc., and achieve the effect of promoting growth and reproduction and good antibacterial ability

Pending Publication Date: 2021-11-05
山东京青农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the obstacle of continuous cropping of old ginseng land has become a major technical problem that has plagued the development of ginseng industry
The growing environment of Panax notoginseng is special, the planting area is limited, and there are serious continuous cropping obstacles
Continuous cropping shows that almost all plants die, and shortening the rotation period shows severe disease and low seedling preservation rate, resulting in low yield and poor quality
[0004] At present, the easiest way to solve the obstacle of continuous cropping is to rotate crops and plant in different pla

Method used

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  • Bacillus subtilis microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1 Preparation of inocula embodiment

[0024] (1) activating species: The cryopreserved JQ-0101D Bacillus subtilis strain was inoculated on LB solid medium and cultured at 36 ℃ 36h; single colony was picked to turn on an LB slant medium, cultured at 36 ℃ 20h , with medium surface of cells as inoculum aseptically washed with water.

[0025] (2) Preparation of a seed liquid: according to 10% (volume percent) inoculum of the seed culture medium access procedure

[0026] Inoculum (1) Preparation of the rotational speed at 220 rpm, temperature 36 ℃, shaking culture for 20 h, as a seed liquid.

[0027] Preparation of Seed Ye two 3): the seed medium access procedure of 3% (volume percent) of the inoculum

[0028] (2) preparing a seed liquid, an aeration amount of 1 .0vvm, stirring speed at 220 rpm, incubation time 20h. As a secondary seed liquid.

[0029] The composition and the weight percent of seed medium: 2% glucose, 1.5% peptone, 1% yeast extract, beef extract 0.7%, Na...

Example Embodiment

[0033] Example 2 Detection antimicrobial

[0034] The intermediate plate with the separator, 200μL prepared above while good Pipette 10 9 CFU / ml of Bacillus subtilis fermentation broth on an LB medium, a coating bar coating even after sterilization, until the surface is dry; while three kinds of pathogens were inoculated on PDA medium, the plates were placed 25 ℃ under cultivation. 3 sets of parallel, coating LB medium sterile saline as control, antibacterial effect observed after 72h.

[0035] The results are shown in Table 1

[0036] Pathogens

[0037] Inhibitory effect

[0038]

[0039] Note: + weak effect of suppressing the colony radius inhibition rate of 10% or less, the influence of fungal mycelial growth; ++ significant antibacterial effect, between the radius colony inhibition rate of 10% -20%; +++ significant inhibitory effect, inhibition of colony radius rate of more than 20%; three replicates averaged.

[0040] Thus, the antibacterial agents of the most obvious eff...

Example Embodiment

[0041] Example 3 acting acidocaldarius (family) (qualitative test)

[0042] In Citrobacter soil acidocaldarius (KLA) represents, respectively, the following two operations, do three sets of parallel:

[0043] 1) was inoculated into MRS basal medium directly shaker, after 8h, detecting OD600 = 0.6;

[0044] 2) Take JQ-0101D fermentation broth, mycelium was removed by filtration, as an alternative to water MRS basal medium, other components of the same, according to the medium was improved 5% inoculation, inoculation Citrobacter, after shaking 8h, the detection OD600 = 0.8.

[0045] Thus, Bacillus subtilis fermentation product JQ-0101D can promote the propagation of bacilli acid.

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Abstract

The invention provides a bacillus subtilis microbial agent, and belongs to the technical field of microorganisms. Specifically, bacillus subtilis is JQ-0101D and is preserved in the China General Microbiological Culture Collection Center, the address of the preservation unit is No. 1 Courtyard, West Beichen road, Chaoyang District, Beijing, the preservation time is March 19, 2021, and the preservation registration number is CGMCC No. 22039. The bacillus subtilis is cultured and fermented to form the microbial agent, and the microbial agent can effectively inhibit pythium, phytophthora and fusarium, has a good biocontrol effect on root rot of araliaceae ginseng plants, and has a certain promoting effect on growth and reproduction of acidobacteria.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus subtilis inoculant and its preparation method and application. Background technique [0002] Continuous cropping obstacle refers to the abnormal growth and development of crops caused by continuous cultivation of the same crop or related crops on the same soil. Symptoms are generally poor growth and development, decreased yield and quality, and in extreme cases, local dead seedlings, no seedlings or vigorous seedlings; most of the affected plants have browning, reduced branches, low vigor, and narrow distribution range, resulting in water absorption. , The capacity of nutrients is reduced. [0003] Ginseng and American ginseng are strictly forbidden to be continuously used as medicinal plants. If ginseng is continuously planted in heavy stubble land, the survival rate of ginseng seedlings will generally drop to below 30% after the second year, and the fibrous r...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCC12N1/20A01N63/22
Inventor 郭中信耿进华尤龙王延刚
Owner 山东京青农业科技有限公司
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