Preparation and application of daphne genkwa extract

The technology of extract and daikon flower is applied to the preparation and application field of fenugreek flower extract, which can solve the problems of bacteria resistance and environmental pollution, and achieve the effects of excellent antibacterial ability, excellent antibacterial range and improving extraction efficiency.

Inactive Publication Date: 2021-08-20
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the prevention and treatment of foodborne diseases in my country is mainly based on chemical drugs such as ant

Method used

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  • Preparation and application of daphne genkwa extract
  • Preparation and application of daphne genkwa extract
  • Preparation and application of daphne genkwa extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of Daphne genkwa extract

[0034] Grind the dried genkwa into powder and pass through a 20-mesh sieve. 1 liter of 80% ethanol was added to 100 grams of Daphne genkwa powder, and ultrasonic extraction was performed at 40° C. and 480 W for 1 hour. Centrifuge at 900 g for 10 minutes at room temperature, remove the supernatant, and add 1 liter of 80% ethanol to the residue for re-extraction for 1 hour. The supernatant was evaporated and concentrated in a rotary evaporator at 40°C to 1 / 5 of the original volume, and the concentrated extract was frozen at -80°C overnight and then dried in a vacuum freeze dryer to obtain Daphne genkwa extract powder. The yield of Daphne genkwa extract powder of the present invention is 13.7%.

Embodiment 2

[0035] Embodiment 2: the preparation of Daphne genkwa extract

[0036] Grind the dried genkwa into powder and pass through a 20-mesh sieve. Add 1 liter of 80% ethanol to 100 grams of Daphne genkwa powder, and ultrasonically extract at 50° C. for 1 hour at 480 W. Centrifuge at 900 g for 10 minutes at room temperature, remove the supernatant, and add 1 liter of 80% ethanol to the residue for re-extraction for 1 hour. The supernatant was evaporated and concentrated to 1 / 4 of the original volume in a rotary evaporator at 40°C, and the concentrated extract was frozen at -80°C overnight and then dried in a vacuum freeze dryer to obtain Daphne genkwa extract powder. The yield of Daphne genkwa extract powder of the present invention is 15.3%.

Embodiment 3

[0037] Embodiment 3: Preparation of Daphne genkwa extract

[0038] Grind the dried genkwa into powder and pass through a 40-mesh sieve. Add 2 liters of 80% ethanol to 100 grams of Daphne genkwa powder, and assisted ultrasonic extraction at 60°C and 480W for 2 hours. Centrifuge at 1000 g for 15 minutes at room temperature, remove the supernatant, and re-extract the residue by adding 2 liters of 80% ethanol for 2 hours. The supernatant was evaporated and concentrated to 1 / 4 of the original volume in a rotary evaporator at 40°C, and the concentrated extract was frozen at -80°C overnight and then dried in a vacuum freeze dryer to obtain Daphne genkwa extract powder. The yield of the genkwa genkwa extract powder of the present invention is 20%.

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Abstract

The invention discloses preparation and application of a daphne genkwa extract, and belongs to the technical field of traditional Chinese medicine extraction and separation. Main antibacterial components in the daphne genkwa extract disclosed by the invention comprise scutellarin, tiliroside, apigenin, hydroxygenkwanin and genkwanin. Research on the antibacterial effect of the daphne genkwa extract shows that the daphne genkwa extract has excellent antibacterial ability on two common gram-positive bacteria (listeria monocytogenes and staphylococcus aureus), the daphne genkwa extract can significantly reduce the listeria monocytogenes and staphylococcus aureus growing on the surfaces of celery leaves, and it is proved that the daphne genkwa extract can be used for fresh-keeping storage of leaf vegetables, bacterial loads in the leaf vegetables are reduced, and food poisoning or food-borne diseases caused by the bacteria are prevented.

Description

technical field [0001] The invention relates to the preparation and application of Genkwa extract, and also relates to the antibacterial effect of Genkwa extract and identification of its main antibacterial components. Background technique [0002] Food safety is the foundation, and food safety is closely related to people's livelihood. Food pollution caused by microorganisms is the main problem of food safety in my country, and bacterial pollution is the main body of microbial pollution. According to WHO statistics, 70% of the global patients caused by food-borne pathogens are caused by contamination of food and drinking water by different pathogenic microorganisms. In recent years, the incidence of global food-borne diseases has been increasing year by year, and the harm caused by them has become increasingly serious. It has become the biggest threat to food safety. Vegetables and fruits may be affected by soil pathogenic bacteria (such as Listeria monocytogenes, Clostrid...

Claims

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Application Information

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IPC IPC(8): A61K36/83G01N30/02G01N30/06G01N30/36G01N30/72A61P31/04A61K133/00
CPCA61K36/83A61P31/04G01N30/02G01N30/06G01N30/7233G01N30/36A61K2236/15A61K2236/333A61K2236/53A61K2236/51
Inventor 高海德金杲云林子涵徐义娟法哈卡比尔
Owner SHANGHAI JIAO TONG UNIV
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