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Application of transglutaminase in medicine for inhibiting or delaying myopia

A technology of transglutaminase and glutaminase, applied in the direction of transferase, drug combination, acyltransferase, etc., can solve the problems of scleral reinforcement surgery, such as controversy, large disturbance, loss of matrix, etc., and improve biomechanical properties. , Increase connection density, inhibit the effect of eye axis extension

Pending Publication Date: 2021-11-12
PEKING UNIV FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the effectiveness of scleral reinforcement surgery is highly controversial
First of all, the scleral reinforcement surgery does not change the structure and mechanical properties of the sclera itself, and pathological features such as abnormal collagen fiber structure and matrix loss caused by myopia still exist; secondly, although autologous fascia lata and allogeneic (cadaveric) sclera can be selected , bovine pericardium and other materials, but it is still not a particularly ideal reinforcement material; moreover, as a traumatic operation, posterior scleral reinforcement surgery has a large disturbance to the eye and orbit, which may include A series of problems such as retinal detachment, orbital hemorrhage, eye infection, eye movement restriction, etc., the long-term curative effect is not optimistic, and the sclera of many patients is still lengthening, which means that the surgery is not easy to achieve the expected effect

Method used

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  • Application of transglutaminase in medicine for inhibiting or delaying myopia
  • Application of transglutaminase in medicine for inhibiting or delaying myopia
  • Application of transglutaminase in medicine for inhibiting or delaying myopia

Examples

Experimental program
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Effect test

Embodiment 1

[0025] This embodiment provides an experiment in which transglutaminase is used to improve the biomechanical properties of rabbit sclera, including,

[0026] Take 10 rabbits, take the right eye as the observation object, as the treatment group, and the contralateral eye (left eye) as the control group. Inject 0.15 ml of PBS solution containing 1 U / mL transglutaminase under the Tenon capsule of the right eye of the rabbit, leave the left eye untreated, and raise it normally for 14 days. The experimental animals were sacrificed by injecting air into the marginal ear vein, and the bilateral eyeballs of the experimental animals were taken out to make a sclera strip of 4mm×14mm, and the sclera was measured in the animal experiment in vivo. Adopt micro-mechanical tester (Shimadzu EZ-Test), the sclera strip carries out tensile test, records the required stretching force of sclera strip under the degree of deformation of 5% and 10% respectively, obtains the modulus of elasticity of sc...

Embodiment 2

[0032] This embodiment provides the experiment that transglutaminase is used to improve the biomechanical properties of isolated porcine sclera, including,

[0033] The in vitro animal experiment was carried out according to the sclera strip with a specification of 14mm×4mm. 40 isolated pig eyes were taken within 24 hours, and 2 sclera strips in symmetrical parts were taken from each eyeball, and one sclera strip was randomly selected as the treatment group. Soak it in PBS solution of 1U / mL transglutaminase solution for 1h to carry out cross-linking. figure 1 Then adopt the micro-mechanical tensile instrument (Shimadzu EZ-Test) to test the mechanical properties of the sclera strip, and the sclera strip carries out the tensile test, and records the tensile force required by the sclera strip under the degree of deformation of 5% and 10% respectively. The stretching force was used to obtain the elastic modulus (E) of the sclera. The test results are shown in Table 2; wherein, in...

Embodiment 3

[0037] This example provides an experiment on the histological structure of the eyeball wall under the action of transglutaminase, including,

[0038] Take 10 isolated pig eyes within 24 hours, a total of 10 samples, and divide them into observation group and control group, with 5 samples in each group, soak the eyeballs in the observation group and control group in different soaking solutions for 0.5 h for cross-linking, the cross-linking temperature was 37°C, and then the eyeball was taken out, and the posterior sclera tissue was taken out, and the collagen fiber density of the sclera tissue was observed after Masson's trichrome staining. Among them, the eyeballs of the observation group were soaked in PBS solution of 1 U / mL transglutaminase solution, and the eyeballs of the control group were soaked in PBS solution only. figure 2 In, a and c, it can be seen that the collagen fibers of the sclera tissue of the observation group are more compact (see 1 in a and 3 in c), and ...

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Abstract

The invention belongs to the technical field of myopia treatment and particularly relates to application of transglutaminase in a medicine for inhibiting or delaying myopia. According to the application, the transglutaminase acts on surfaces of sclerae, and by promoting the generation of isopeptide bonds among collagen fiber amino acids, the density of connection among collagen molecules can be increased and the biomechanical properties of the sclerae can be improved. The sclerae are main components of eyeball walls, and after mechanical properties of the sclerae are improved, the firmness of the eyeball walls can be greatly improved, so that the growth of axis oculi is limited, and thus, the aim of controlling and treating the myopia is achieved. The transglutaminase can be used for improving the mechanical properties of the sclerae by 50%-200%, and no toxic or side effect on other parts of eyes is observed.

Description

technical field [0001] The invention belongs to the technical field of myopia treatment, and in particular relates to the application of transglutaminase in medicines for inhibiting or delaying myopia. Background technique [0002] Myopia is one of the diseases with the highest incidence rate in humans, and the prevention and treatment of myopia is one of the biggest challenges facing global public health. Among them, high myopia is extremely harmful, and patients may develop serious complications and lead to blindness. [0003] The definition of myopia is that after the refraction of the eye, the focus of the object image falls in front of the retina. According to the cause of myopia, it can be divided into two types: refractive myopia and axial myopia. Refractive myopia is caused by the excessive refractive power of the cornea or lens of the eye, causing the object image to be located in front of the retina; the treatment method is to change the refractive properties of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/45A61P27/10
CPCA61K38/45C12Y203/02013A61P27/10
Inventor 吴元
Owner PEKING UNIV FIRST HOSPITAL
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