Quantitative detection method for index components in standard decoction of Xiaoxuming decoction and application thereof

A quantitative detection method, the technology of Xiaoxuming Decoction, applied in the direction of measuring device, material separation, analysis of materials, etc., can solve the problems of lack of systematic research on the overall quality control of Xiaoxuming Decoction

Active Publication Date: 2021-11-12
SHINEWAY PHARMA GRP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The clinical efficacy and pharmacological effects of Xiaoxuming Decoction have been scientifically verified, and the research on its active ingredients has also made progress, but there is still a lack of systematic research on the overall quality control of Xiaoxuming Decoction

Method used

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  • Quantitative detection method for index components in standard decoction of Xiaoxuming decoction and application thereof
  • Quantitative detection method for index components in standard decoction of Xiaoxuming decoction and application thereof
  • Quantitative detection method for index components in standard decoction of Xiaoxuming decoction and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] The embodiment of the present invention provides a quantitative detection method for the index components in Xiaoxuming decoction standard decoction, the index components are ginsenoside Rg 1 , Ginsenoside Re, Baicalin, Liquiritin, Glycyrrhizic Acid, Hypoaconitine, Benzoyl Aconitine, Benzoyl Hypoaconitine, Benzoyl Neoaconitine, Paeoniflorin, Ferulae acid, acteoside, 5-O-methylvisamigoside, amygdalin, tetrandrine, fangchinoline and 8-gingerol. Use ultra-high performance liquid chromatography / mass spectrometry to detect the index components in Xiaoxuming decoction standard decoction. The chromatographic conditions of ultra-high performance liquid chromatography are:

[0080] Chromatographic column: Shimadzu Shim-pack XR-ODS Ⅲ UHPLC column, the specification is 2.0×50mm, 1.6μm;

[0081] Mobile phase A is 0.002mol / L ammonium acetate aqueous solution containing 0.2% v / v formic acid, mobile phase B is acetonitrile, and linear gradient elution is carried out. The program of l...

Embodiment 2

[0092] The embodiment of the present invention provides a kind of quality control method of Xiaoxuming decoction standard decoction, with ginsenoside Rg 1 , Ginsenoside Re, Baicalin, Liquiritin, Glycyrrhizic Acid, Hypoaconitine, Benzoyl Aconitine, Benzoyl Hypoaconitine, Benzoyl Neoaconitine, Paeoniflorin, Ferulae acid, acteoside, 5-O-methylvisamidol, amygdalin, tetrandrine, tetrandrine, 8-gingerol, cinnamic acid, ephedrine and pseudoephedrine as index components. With the quantitative detection method among the embodiment 1 to ginsenoside Rg 1 , Ginsenoside Re, Baicalin, Liquiritin, Glycyrrhizic Acid, Hypoaconitine, Benzoyl Aconitine, Benzoyl Hypoaconitine, Benzoyl Neoaconitine, Paeoniflorin, Ferulae acid, acteoside, 5-O-methylvisamidol glycoside, amygdalin, tetrandrine, tetrandrine and 8-gingerol were quantitatively detected, and ultra-high performance liquid chromatography / mass spectrometry Determination of cinnamic acid, ephedrine and pseudoephedrine in standard decoction...

Embodiment 3

[0112] The embodiment of the present invention has carried out methodological verification to the detection method of above embodiment 1, 2.

[0113] 1. Experimental materials

[0114] The name, base source, batch number, origin and other information of the medicinal herbs used in this experiment are shown in Table 3.

[0115] Table 3 The information of the medicinal taste used in the methodological verification

[0116] name base source batch number place of origin ginseng Dried roots and rhizomes of Panax ginseng C.A.Mey. 1709272 Jilin Jingyu Scutellaria baicalensis Dried root of Scutellaria baicalensis Georgi HQ180324 Shanxi Wenxi licorice Dried roots and rhizomes of Glycyrrhiza uralensis Fisch. 17111412 Gansu Longxi Cinnamon Dried bark of Cinnamomum cassia Presl RG1809191 Zhaoqing, Guangdong ephedra Dried herbaceous stems of Ephedra sinica Stapf MH180321 Chifeng, Inner Mongolia aconite Processed pr...

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Abstract

The invention relates to the technical field of traditional Chinese medicine component detection, in particular to a quantitative detection method for index components in standard decoction of Xiaoxuming decoction and application thereof. According to the present invention, the ultra-high performance liquid chromatography / mass spectrometry analysis method is adopted to detect the index components in the standard decoction of Xiaoxuming decoction so that the high precision, the high accuracy, the high stability and the high reproducibility are realized, the chromatographic peaks of the index components such as ginsenoside Rg1, ginsenoside Re, baicalin, liquiritin, glycyrrhizic acid, hypaconitine, benzoyl aconitine and the like can be effectively separated, therefore, various index components in the standard decoction of the Xiaoxuming decoction can be rapidly and accurately detected.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicine components, and in particular relates to a quantitative detection method and application of index components in Xiaoxuming decoction standard decoction. Background technique [0002] The classic famous prescription "Xiao Xuming Decoction" comes from Tang Sun Simiao's "Preparation for Emergency Prescriptions". 7.0g, white peony 14.0g, Chuanxiong 14.0g, Fangfeng 21.0g, bitter almond 14.0g, Fangji 14.0g, ginger 70.0g. The active ingredients of Xiaoxuming Decoction mainly include flavonoids (mainly from Scutellaria baicalensis and licorice), alkaloids (mainly from aconite, ephedra, and Fangji), saponins (mainly from ginseng and licorice), and non-saponin terpenoids (mainly from peony) And chromones (mainly from wind), in addition to fatty acids, fatty alcohols, cyanogenic glycosides (mainly from bitter almonds), phthalides (mainly from Chuanxiong) and so on. Xiao Xu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/72
CPCG01N30/02G01N30/34G01N30/72Y02A50/30
Inventor 黄熙凯刘广学蔡少青孙长清侯金才
Owner SHINEWAY PHARMA GRP LTD
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