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Preparation method and application of PHBV-fibroin sustained and controlled release film containing anti-fibrosis nanoparticles

A nanoparticle, anti-fibrosis technology, applied in the field of medicine, can solve the problems of poor hydrophilicity, limited biocompatibility of PHBV, and unsatisfactory applicability, and achieve the effect of good biocompatibility

Pending Publication Date: 2021-11-16
爱尔眼科医院集团股份有限公司长沙爱尔眼科医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the present invention provides a preparation method and application of a PHBV-silk fibroin sustained-release film containing anti-fibrosis nanoparticles, which solves the limited biocompatibility of PHBV and its relatively poor Hydrophilicity and unsatisfactory applicability in applications

Method used

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  • Preparation method and application of PHBV-fibroin sustained and controlled release film containing anti-fibrosis nanoparticles
  • Preparation method and application of PHBV-fibroin sustained and controlled release film containing anti-fibrosis nanoparticles

Examples

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Embodiment 1

[0037] like figure 1 As shown, the embodiment of the present invention provides a preparation method of a PHBV-silk fibroin slow-release film containing anti-fibrosis nanoparticles, comprising the following preparation steps:

[0038] S1. Extract silk fibroin and post-process silk fibroin: clean the surface of natural silkworm cocoons and cut them into pieces, add them to 0.02M NaCO3 solution; A. Heat and boil for 30 minutes; B. Discard the NaCO3 liquid and wash the silk with deionized water vegan fiber;

[0039] Repeat steps A and B 3 times to obtain silk fibroin fibers;

[0040] For post-treatment of silk fibroin fiber, add silk fibroin to 9.3M LiBr solution to obtain 25% (w / v%) silk fibroin-LiBr solution, heat in a water bath at 60°C for 4 hours, and dissolve the mixed solution after the silk fibroin fiber is completely dissolved Put it into a dialysis bag, dialyze in deionized water for 3 days, replace the deionized water every 8 hours, centrifuge the solution after dial...

Embodiment 2

[0047] like figure 1 As shown, the embodiment of the present invention provides a preparation method of a PHBV-silk fibroin slow-release film containing anti-fibrosis nanoparticles, comprising the following preparation steps:

[0048] S1. Extract silk fibroin and post-process silk fibroin: clean the surface of natural silkworm cocoons and cut them into pieces, add them to 0.02M NaCO3 solution; A. Heat and boil for 30 minutes; B. Discard the NaCO3 liquid and wash the silk with deionized water vegan fiber;

[0049] Repeat steps A and B 3 times to obtain silk fibroin fibers;

[0050] For post-treatment of silk fibroin fiber, add silk fibroin to 9.3M LiBr solution to obtain 25% (w / v%) silk fibroin-LiBr solution, heat in a water bath at 60°C for 4 hours, and dissolve the mixed solution after the silk fibroin fiber is completely dissolved Put it into a dialysis bag, dialyze in deionized water for 3 days, replace the deionized water every 8 hours, centrifuge the solution after dial...

experiment example

[0057] The PHBV-silk fibroin film containing drug-loaded nanoparticles proposed by the present invention and pure PHBV, PHBV-silk fibroin, and the PHBV film containing drug-loaded nanoparticles are observed with a scanning electron microscope, and the observation results (see figure 2 );

[0058] Finally, it is found that the performance of the PHBV-silk fibroin film containing drug-loaded nanoparticles proposed by the present invention is significantly better than that of pure PHBV, PHBV-silk fibroin, and PHBV films containing drug-loaded nanoparticles.

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Abstract

The invention provides a preparation method and application of a PHBV-fibroin sustained and controlled release film containing anti-fibrosis nanoparticles, and relates to the field of medicines. The preparation method of the PHBV-fibroin sustained and controlled release film containing the anti-fibrosis nanoparticles comprises the following preparation steps: S1, extracting fibroin, and carrying out post-treatment on the fibroin; s2, preparing a composite nanoparticle / PHBV fibroin slow-control membrane: weighing PHBV and fibroin powder, dissolving the weighed PHBV and fibroin powder into an HFIP solution, weighing drug-loaded nanoparticles RSG / Pd@ZIF-8, and dissolving the RSG / Pd@ZIF-8 into HFIP, and adding the HFIP solution containing nanoparticles into the PHBV-fibroin-HFIP solution; s3, removing the organic solvent. The performance of the anti-fibrosis sustained-release membrane is optimized, so that the anti-fibrosis sustained-release membrane has excellent mechanical properties and biocompatibility. Compared with a pure PHBV sustained-release membrane, due to better biocompatibility, the anti-fibrosis sustained-release membrane has wider anti-fibrosis adaptability diseases, is not limited to anti-filtration channel cicatrization after glaucoma operation, and is suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method and application of a PHBV-silk fibroin sustained and controlled release film containing anti-fibrosis nanoparticles. Background technique [0002] Fibrosis is the final common pathological result of many chronic immune diseases and chronic inflammatory diseases. Fibrosis can affect any organ and is responsible for 45% of deaths in industrialized countries. There are common fibrotic diseases in various organs and tissues, such as cardiac fibrosis, liver fibrosis, pulmonary fibrosis, joint fibrosis, and skin fibrosis, etc. With the process of industrialization in our country, the health care expenditure caused by fibrosis will further increase. Our previous research found that rosiglitazone can control the scarring (fibrosis) of the filtration channel and developed a PHBV sustained-release membrane containing rosiglitazone drug-loaded nanoparticles for long-...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/22A61L27/54D04H1/728
CPCA61L27/18A61L27/54A61L27/227D04H1/728C08L67/04
Inventor 段宣初张凤刘海蓉周灯明朱文祥赵阳
Owner 爱尔眼科医院集团股份有限公司长沙爱尔眼科医院