Sampling gun integrating liquid drop sampling, sample treatment and detection and method thereof

Abstract

The invention provides a sampling gun integrating liquid drop sampling, sample treatment and detection and a method thereof. The sampling gun comprises a sampling gun body, and a negative pressure generation structure, a plug-in detection chip, a temperature control module and an oil phase storage cavity which are sequentially arranged in the sampling gun body from the rear end to the front end, and sample channels for conveying sample liquid drops are arranged in the temperature control module and the oil phase storage cavity. The sampling run also comprises a liquid drop sampling gun head operably connected to the front end of the sampling gun body, and the liquid drop sampling gun head comprises an outer-layer pipeline extending axially, an inner-layer capillary tube fixed in the outer-layer pipeline and coaxially extending with the outer-layer pipeline, and ahydrophilic filtering structure fixed at the front end of the outer-layer pipeline, and a certain gap is kept between the hydrophilic filtering structure and the front end of the inner-layer capillary tube. According to the invention, a simple, efficient, reliable, rapid, high-throughput and high-sensitivity biological sample detection method is provided, and an effective technical means and a tool are expected to be provided for instant fixed-point rapid diagnosis of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of liquid sampling devices, in particular to a sampling gun integrating liquid droplet sampling, sample processing and detection and a method thereof. Background technique [0002] The detection of pathogenic bacteria is of great significance to human health and life safety. Existing detection methods for pathogenic bacteria require high detection environment, long time period, low throughput, and large equipment volume. Therefore, a closed, fast, high-throughput, portable and miniaturized real-time, fixed-point detection system is the best way to detect pathogenic bacteria. The direction of technology development. [0003] Droplet digital LAMP is a brand new nucleic acid absolute quantification technology that has emerged in the past two years and does not require a standard curve. Because it can accurately detect the concentration of pathogenic bacteria in a low-cost droplet and constant temperature environment, it...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a sampling gun and its method integrating liquid drop sampling, sample processing and detection, so as to solve the problem that the existing pathogenic bacteria detection technology is difficult to ensure a closed sampling environment, slow detection, low throughput, And the problem of huge testing equipment

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