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Transdermal total absorption type anti-aging nano functional mask based on superoxide dismutase

An anti-aging and facial mask technology, applied in the field of biological enzymes, can solve the problems of increased risk, increased product cost, and unstable activity of animal-derived blood products, and achieve the effects of wide application value, economic value, and improved thermal stability

Pending Publication Date: 2021-11-19
深圳市不二生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the 1980s in my country, the applied research on SOD has mainly focused on the extraction and purification of animal and plant SOD. Most of the domestic SOD products are prepared from animal blood, but due to limited raw materials and hygienic safety, etc. The output of products is limited, especially with the frequent reports of mad cow disease, bird flu, foot-and-mouth disease and other vicious infectious diseases around the world, the risk of animal-derived blood products increases, and the increase in purity requirements also increases the cost of products, especially due to the impact of mad cow disease After the European Union stipulated that the use of bovine blood SOD as a food additive was banned from January 1997, the use of genetic engineering is an effective way to broaden the source of SOD enzymes, reduce costs and obtain SOD with natural activity
However, there are problems such as low yield and unstable activity of SOD enzyme in genetic engineering, and when it is applied to cosmetics, it needs to increase its stability to a wider range of temperatures to facilitate transportation. Therefore, it is very important to develop a SOD enzyme that can overcome the above-mentioned unfavorable factors. necessary

Method used

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  • Transdermal total absorption type anti-aging nano functional mask based on superoxide dismutase
  • Transdermal total absorption type anti-aging nano functional mask based on superoxide dismutase
  • Transdermal total absorption type anti-aging nano functional mask based on superoxide dismutase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: Mutant enzyme thermostability

[0014] The Cu, Zn-SOD enzyme (NP_012638.1) from Saccharomyces cerevisiae was obtained from NCBI, and four key amino acid sites (S39G, H47A, G86W, H131Q) on its active site were selected for mutation research .

[0015] A one-step reverse PCR method is used to construct Cu, Zn-SOD enzyme site-directed transformation mutants, and after sequencing verification, the correctly constructed site-directed mutation transformation plasmid is transformed into host cells.

[0016] The correctly constructed mutation sites (group 1: S39G+H47A, group 2: S39G+G86W, group 3: S39G+H131Q, group 4: H47A+G86W, group 5: H47A+H131Q, group 6: G86W+H131Q ) single colony was isolated by streaking on a plate, picked and cultured in LB medium (Kan) for 12 hours, inoculated into TB fermentation medium (Kan) for fermentation and cultured for 26 hours, and the fermentation supernatant was obtained for each mutant recombinant enzyme Heat treatment at pH ...

Embodiment 2

[0017] Example 2: Antioxidant Activity Verification

[0018] Test samples: wild-type Cu, Zn-SOD enzyme and H47A+G86W double mutant Cu, Zn-SOD enzyme were mixed with ultrapure water and diluted into a test solution with a mass fraction of 5%.

[0019] Hydroxyl radical scavenging experiment: using conventional spectrophotometry in this field, measure the absorbance value of each group of mixed solutions at 536 nm, denoted as As, and use distilled water instead of the sample solution as the blank group to measure the absorbance value, denoted as Ab, and distilled water Replace H 2 o 2 As the damage group, measure its absorbance value An, and the hydroxyl radical scavenging rate of the test sample is calculated according to the following formula: hydroxyl radical scavenging rate (%)=[(As-An) / (Ab-An)]×100%, take three times The average value of the measurements. The test results are shown in Table 1.

[0020] DPPH free radical scavenging experiment: Take 3mL of DPPH-absolute et...

Embodiment 3

[0024] Embodiment 3 Mutant product skin elasticity and moisturizing function test

[0025] The above-mentioned H47A+G86W double mutant Cu, Zn-SOD enzyme is prepared as a skin care essence, and the skin care essence includes the following components by mass percentage:

[0026] H47A+G86W double mutant Cu, Zn-SOD enzyme 5%, glycerin 5%, hyaluronic acid 1.5%, L-vitamin C 1%, vitamin E 5%, urea 1%, propylene glycol 0.5%, and the balance is deionized water.

[0027] Control group 1 is to replace H47A+G86W double mutant Cu, Zn-SOD enzyme with wild-type Cu, Zn-SOD enzyme.

[0028] Control group 2 is without adding Cu, Zn-SOD enzyme.

[0029] Test population: select 60 healthy volunteers with an average age of 40±2, and randomly divide them into three groups, 20 people in each group, apply the above samples on the face sooner or later, and the subjects cannot apply any other cosmetics on the test site during the experiment .

[0030] testing method:

[0031] A moisture tester (Cor...

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Abstract

The invention provides a novel superoxide dismutase mutant, and the mutant shows the purposes of improving skin aging and reducing wrinkles, and has a wide application prospect. Furthermore, the invention also provides a transdermal total absorption type anti-aging nano functional mask containing the mutant.

Description

technical field [0001] The invention belongs to the field of biological enzymes, and more specifically, the invention relates to a mutant superoxide dismutase and a transdermal full-absorption anti-aging nano-functional facial mask based on the superoxide dismutase. Background technique [0002] The two main causes of skin aging are due to sun exposure and the intrusion of active oxygen. Active oxygen removes foreign substances by decomposing them in the process of removing foreign substances that have entered the body. However, while playing a positive role, if excessive active oxygen is produced due to various reasons, it will not only damage its own cell tissue, but also combine with unsaturated fatty acids in the body to form lipid peroxide. In particular, it combines with proteins and lipids in the skin to form oxides, leading to skin aging, and also plays a strong role in the oxidation process of melanin, causing pigmentation. [0003] Superoxide dismutase (Superoxid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02A61K8/66A61Q19/08
CPCC12N9/0089A61K8/66A61Q19/08C12Y115/01001
Inventor 赵聪司威
Owner 深圳市不二生物科技有限公司
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