Curculigo orchioides glycosyl transferase Co84A-471 gene and application thereof in preparation of orcinol glucoside
A technology of orcinol glucoside and co84a-471, which is applied in the biological field, can solve the problems of long growth cycle, complex extraction process, low yield of orcinol glucoside, etc., and achieves the effect of less by-products
Active Publication Date: 2021-11-19
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology
Orcinol glucoside is mainly distributed in Curculigo curculiculi and other Curculigo genus plants, and its content is higher in the rhizomes of Curculigo curculigo, but these plants have strict requirements on the cultivation environment, long growth cycle, and low yield; in addition, they also have the ability to extract The technical process is more complicated, the separation rate is low and other deficiencies, so that the yield of orcinol glucoside is low, resulting in high market price
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Embodiment 1
[0033] Mining and in vitro verification of UGT candidate gene glycosyltransferase Co84A-471
[0034] The expanded rhizomes and leaves of Curculigo curculigo were used as materials, and samples were sent to Guangzhou Kidio Biotechnology Co., Ltd. for transcriptome sequencing. First, according to the annotation results of transcriptome data, all 101 candidate glycosyltransferases were screened out. Secondly, combining the characteristics of glycosyltransferase genes with known functions, including Arabidopsis UGT84A1, grape GT2, etc., and then combining the content distribution characteristics of orcinol glucoside in the rhizome of Curculigo curculigo, and the gene expression level for comprehensive analysis , 6 possible candidate genes were initially screened out. After a series of work such as cloning, homologous recombination, prokaryotic expression, in vitro enzymatic reaction, high performance liquid phase detection and LC / MC identification of the candidate gene, it was fi...
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Abstract
The invention discloses a Curculigo orchioides glycosyl transferase Co84A-471 gene and an application of the Curculigo orchioides glycosyl transferase Co84A-471 gene in the preparation of orcinol glucoside. The nucleotide sequence of the curculigo orchioides glycosyl transferase Co84A-471 gene is as shown in SEQ ID NO: 1. By taking orcinol and a glycosyl donor UDP-glucose as raw materials, primary glycosylation is conducted on hydroxyl groups on C3 or C5 sites of the orcinol under the catalysis of curculigo orchioides glycosyl transferase obtained by coding a curculigo orchioides glycosyl transferase Co84A-471 gene, so that orcinol glucoside is generated. By in-vitro synthesis of the orcinol glucoside, the controllability is high, the requirement for raw material planting can be reduced, agricultural land is saved, the produced product is single, and separation and purification of the orcinol glucoside in the later period are facilitated. The Curculigo orchioides glycosyl transferase Co84A-471 gene is used as a key gene for biosynthesis of orcinol glucoside, and can also be used for breeding research of plants such as Curculigo orchioides and the like.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gene of Curculigo glycosyltransferase Co84A-471 and its application in the preparation of orcinol glucoside. Background technique [0002] Curculigo orchioides Gaertn is a medicinal plant of the genus Curculigo in the family Amaryllidaceae, and its rhizomes are used as medicine. Phenolic components are widely distributed in Curculigoside and are the main components. At present, a variety of phenolic glycosides have been isolated and identified from the rhizome of Curculigoside, and its components are Curculigoside and Orinol glucoside (Orinol glucoside, OG) is the main active ingredient of curculigo. [0003] Orcinol glucoside has a variety of significant pharmacological activities, such as anti-oxidation, anti-osteoporosis, anti-depression, anti-anxiety and immune regulation, etc. It has certain cytotoxic activity (Qiang Mingmin et al., Research progress on pharmacok...
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IPC IPC(8): C12N9/10C12N15/54C12N15/70C12N1/21C12P19/44C12N15/11C12R1/19
CPCC12N9/1051C12N15/70C12P19/44
Inventor 陈碧环刘祥宇向贵生王益娜张广辉杨生超字淑慧
Owner YUNNAN AGRICULTURAL UNIVERSITY