Large-view-field light sheet microscopic imaging system and method based on multi-slit interference illumination

Abstract

The invention provides a large-view-field light sheet microscopic imaging system and method based on multi-slit interference illumination. The large-view-field light sheet microscopic imaging system comprises a Gaussian beam generation module used for generating Gaussian beams; a non-diffraction-like Bessel light sheet generation module which is used for receiving the Gaussian beam, converting the Gaussian beam into a non-diffraction-like Bessel light sheet of which the focal depth range is larger than the Rayleigh distance of the Gaussian beam, projecting the non-diffraction-like Bessel light sheet onto a sample surface and exciting the sample to generate a fluorescence signal; and a detector which is used for collecting fluorescence signals to obtain the image information of the sample. The system is advantaged in that the Gaussian beam is converted into the non-diffraction-like Bessel light sheet with the focal depth range larger than the Rayleigh distance of the Gaussian beam through the non-diffraction-like Bessel light sheet generation module, a sample is excited through the non-diffraction-like Bessel light sheet to generate a fluorescence signal, thickness of an illumination light sheet can be reduced, the focal depth of the illumination light sheet is increased, a side lobe of a Bessel beam is eliminated, background noise is reduced, and the imaging view field and the resolution are both improved.

Description

technical field [0001] The invention belongs to the technical field of fluorescence microscopic imaging, and in particular relates to a large field of view light sheet microscopic imaging system and method based on multi-slit interference illumination. Background technique [0002] Light-sheet fluorescence microscopy is a new type of three-dimensional fluorescence microscopy imaging technology. It adopts a special design in which the excitation light path is perpendicular to the detection light path. It uses a light beam sheet to excite from the side of the sample, and through the microscope objective lens and the detector are perpendicular to each other. Fluorescence images of the illuminated plane are acquired in the direction of the light sheet, enabling 3D tomography of thick samples. Compared with the traditional fluorescence microscopy imaging technology, the light sheet fluorescence microscopy imaging replaces the traditional point scanning imaging method with surface...

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Problems solved by technology

[0005] In view of the deficiencies in the prior art above, the purpose of the present invention is to provide a large field of view light sheet microscopic imaging system and method based on multi-slit interference illumination, which overcomes the inability of the existing light sheet microscopic imaging system to simultaneously improve the field of view and resolution deficiencies of the slice microscope

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