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Oil adjuvant vaccine demulsifier and application thereof

A demulsifier and oil adjuvant technology, which is applied to the field of biphasic oil adjuvant vaccine demulsifier and its preparation, can solve the problems of difficulty in animal detection, irregular data, poor efficiency and effect of demulsifier, and achieve demulsification. The effect of short time, improved recovery, and less use of reagents

Pending Publication Date: 2021-11-23
郑州爱德佳生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a demulsifier for the current animal oil adjuvant vaccine quality inspection, the problem that the animal detection is difficult and the data is irregular, and the efficiency and effect of some existing demulsifiers are not good. Preparation

Method used

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  • Oil adjuvant vaccine demulsifier and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Preparation of simulated biphasic oil adjuvant vaccine emulsion: 200 ml of feline calicivirus antigen (provided by Zhengzhou Aike Biotechnology Co., Ltd., virus preservation number CCTCC NO:V202003), 200 ml of ready-to-use biphasic adjuvant ADJ206 (W / O / W) Provided by Aidejia Biotechnology Co., Ltd. Emulsify according to the instructions, and check the properties of the emulsion to be qualified.

[0017] Demulsification test: In a 15 ml centrifuge tube, take 5 ml of vaccine emulsion, then add 5 ml of chloroform, 0.5 mg (NH 4 ) 2 SO 4 After mixing by hand, centrifuge at room temperature at 4000rpm for 10min. The mixture in the centrifuge tube is divided into two layers, which are the water phase layer and the oil phase layer from top to bottom, as shown in Figure 1, from which it can be clearly seen that It can be seen that the left side is 5ml emulsion control, the right centrifuge tube is 5ml emulsion, 0.5g ammonium sulfate, 5ml chloroform, demulsification and centr...

Embodiment 2

[0020] Take 5ml of the emulsified oil adjuvant vaccine in a centrifuge tube, add 5ml of chloroform, 0.5g of ammonium sulfate or other inorganic salts that can significantly enhance the polarity of water, mix well, and centrifuge at 4000 rpm for 10 minutes in a centrifuge. The resulting upper liquid is the aqueous phase.

Embodiment 3

[0022] Take 10ml of the emulsified oil adjuvant vaccine in a centrifuge tube, add 5ml of chloroform and 0.5g of ammonium sulfate, mix well, and then centrifuge at 3000 rpm for 10 minutes in a centrifuge. The supernatant liquid obtained after centrifugation is the water phase.

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Abstract

The invention discloses a two-phase oil adjuvant vaccine demulsifier. The demulsifier comprises chloroform and ammonium sulfate, the volume ratio of the chloroform to vaccine emulsion is 2: (1-2), and the mass-volume ratio of the ammonium sulfate to the vaccine emulsion is 1: (5-20). The demulsifier can effectively and quickly separate the water phase and the oil phase in a two-phase oil adjuvant vaccine, is short in demulsification time, few in used reagent, simple to operate, low in requirement on operating environment, and capable of being operated at room temperature, the separated water phase is supernatant liquid, the water phase is obvious, the water phase is convenient to extract, and the recovery of the antigen can be obviously improved.

Description

technical field [0001] The invention relates to a two-phase oil adjuvant vaccine demulsifier and its preparation method and application, belonging to the field of biotechnology. Background technique [0002] The quality of efficient and rapid oil adjuvant vaccines has always been the focus of attention. The existing vaccine quality standards stipulate that the efficacy test must use this animal for testing. Since the country implements a 100% enhanced immunization policy, it is difficult to select susceptible ones. Animals are used for testing, and animal testing has high requirements for experimental facilities (BSL3 level laboratory), takes a long time (more than one month), and costs a lot of money. If the serum neutralization test is used to select susceptible animals, it is technically difficult to exclude non-susceptible animals with cellular immunity, and the problem of irregular test data often occurs in practice, which affects the accuracy of the test. Therefore, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D17/04G01N1/28
CPCB01D17/04G01N1/28
Inventor 李少英刘鑫宇乔晶晶
Owner 郑州爱德佳生物技术有限公司