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Application of Pseudomonas perfectomarina in nitrogen removal of salty and fresh water nitrogen-containing sewage

A Pseudomonas, brackish and fresh water technology, applied in the application field of denitrification of brackish and fresh water nitrogen-containing sewage, can solve the problems such as the application limitation of efficient denitrification of tail water

Active Publication Date: 2021-11-23
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is precisely because of the alternation of brackish and fresh water in the aquaculture water that the application of high-efficiency denitrification of tail water is limited to a certain extent. The development of strains with a wide range of salinity and high denitrification efficiency plays an important role in brackish and fresh water aquaculture water.

Method used

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  • Application of Pseudomonas perfectomarina in nitrogen removal of salty and fresh water nitrogen-containing sewage
  • Application of Pseudomonas perfectomarina in nitrogen removal of salty and fresh water nitrogen-containing sewage
  • Application of Pseudomonas perfectomarina in nitrogen removal of salty and fresh water nitrogen-containing sewage

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Embodiment 1

[0038] 1. Sample collection

[0039]Sample collection According to the "mixed sample collection method" in the "Technical Specifications for Soil Environment Monitoring" (HJ / T 166-2004), the plum blossom point sampling method is used to sample at points, and the surface, middle and deep water bodies and sediments are collected from freshwater aquaculture ponds In a sterile sampling bag, refrigerated, transported and stored at 4°C for later use.

[0040] 2. Strain enrichment, isolation and screening

[0041] The sediment samples were inserted into sterile saline, and were dispersed by shaking glass beads for 1 hour, so that the microorganisms were fully suspended in the saline. The above mixed liquid is added to the enriched medium and cultured for 2-3 days. Add 1 mL of NH with a concentration of 5% by mass to the enriched medium every day 4 Cl solution to maintain the culture medium ion concentration. Take 1 mL of the enriched sample, put it into a test tube containing 9...

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Abstract

The invention discloses an application of Pseudomonas perfectomarina in nitrogen removal of salty and fresh water nitrogen-containing sewage. The Pseudomonas perfectomarina WM33 provided by the invention is applied to the field of salt and fresh water nitrogen-containing aquaculture tail water treatment, and the Pseudomonas perfectomarina has broad salinity and can be applied to removal of three inorganic nitrogen elements in the salt and fresh water aquaculture tail water. The application has the characteristics of high denitrification efficiency, high aquatic organism safety, high environmental safety and the like, and has a great application value in the aspect of denitrification treatment of the salt-freshwater aquaculture tail water.

Description

Technical field: [0001] The invention belongs to the field of aquaculture tail water treatment, and in particular relates to the application of Pseudomonas panmarinae in the denitrification of saline and fresh water nitrogenous sewage. Background technique: [0002] Aquaculture tail water treatment is an important factor restricting the green development of aquatic products. body of water as well as High concentrations will have a toxic effect on the aquaculture objects in the water body, and it is also the most important component of the water quality of the aquaculture tail water exceeding the standard. It is of great significance to carry out the application research of biological denitrification. [0003] The total economic volume of aquaculture in Zhuhai City accounts for 70% of the city's agricultural economy. Doumen District, Zhuhai City has a breeding area of ​​188,900 mu of perch, a total output of 245,000 tons of aquatic products, and a total fishery output v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F1/66C02F101/16C02F103/08C02F103/20
CPCC02F3/34C02F3/348C02F2103/08C02F2101/16C02F2101/163C02F2101/166C02F1/66C02F2209/06C02F2209/02C02F2103/20Y02W10/10
Inventor 黄文孙慧明舒琥陈冰陈国建
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI