Tissue culture method of dendrocalamus latiflorus
A technology of tissue culture and hemp bamboo, applied in the field of plant tissue culture, can solve the problems of restricting research and development, natural reproduction is difficult to meet the needs of today's industries, and rapid death
Pending Publication Date: 2021-11-26
JILIN AGRICULTURAL UNIV
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Problems solved by technology
[0003] Although hemp bamboo is a high-quality plant raw material, its natural reproduction is difficult to meet the needs of today's industry
In terms of breeding, the biological characteristics of bamboos such as irregular flowering and rapid death after flowering limit its research and development.
At present, most of the literature reports focus on the cutting propagation and application value analysis of Mazhu, and there are very few literature reports on the tissue culture of Mazhu. In China, Qin Heye et al. Tissue culture research of medicine (Li Haiying, 2011) and other reports about Ma bamboo tissue culture
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Embodiment 1
[0031] A method for tissue culture of Ma bamboo, comprising the steps of:
[0032] 1) Callus formed by twigs: after disinfection, cut the leaves of Bamboo japonica picked in the field into small pieces of about 4 mm × 4 mm, inoculate them on the callus induction medium, and culture them routinely until callus is formed; The callus formation time of the twigs is 10 days; the induction medium of the callus of the bamboo shoots is 0.48~0.52mg / L indolebutyric acid and 8mg / L 2,4-dichlorobenzene The MS solid medium of oxyacetic acid; the sucrose concentration in the MS medium is 31g / L, and the phytogel concentration is 4.1g / L; the culture environment is an independent sterile environment, the pH value is 6.1, and the culture temperature is 23±2℃, the light intensity is 1800-2100Lx, and the daily light is 16h / d.
[0033] 2) callus differentiation into adventitious buds: the callus obtained in step 1) was cut into small pieces of 4mm×4mm, inoculated on the induction medium for differ...
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The invention relates to a tissue culture method of dendrocalamus latiflorus, and belongs to the technical field of tissue culture and rapid propagation of dendrocalamus latiflorus groups of hempleaf bamboos. The propagation method comprises the following steps of (1) forming calluses; (2) differentiating adventitious buds from the calluses; (3) conducting adventitious bud rooting culture; and (4) conducting rooting culture of dendrocalamus latiflorus tissue culture seedlings. According to the propagation method, dendrocalamus latiflorus young branches are selected as explants, so that a large number of high-quality dendrocalamus latiflorus tissue culture seedlings can be obtained within 30 days, and excellent raw materials can be provided for research and application of the dendrocalamus latiflorus quickly, conveniently and massively. The method has important significance in the aspects of protecting natural resources of the dendrocalamus latiflorus, meeting the requirements of cultivation on seedlings, establishing a gene pool and the like.
Description
technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for tissue culture of bamboo shoots. Background technique [0002] Bamboo is a plant of the genus Bambusa in the Poaceae family. This species is the most widely cultivated bamboo species in southern my country. It is 20-30m high, 15-30cm in diameter, and 45-60cm long between nodes. branch. Mazhu is an excellent bamboo for both bamboo shoots and timber. The bamboo shoots taste sweet and have high nutritional value. A large number of dried bamboo shoots and canned bamboo shoots are listed on the market every year and sold overseas; Garden planting has high ornamental value. [0003] Although hemp bamboo is a high-quality plant raw material, its natural reproduction is difficult to meet the needs of today's industries. In terms of breeding, the biological characteristics of bamboos such as irregular flowering and rapid death after flowering limit its research and devel...
Claims
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姚强
Owner JILIN AGRICULTURAL UNIV