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Application of ASS1 or BCKDK inhibitor in preparation of medicine for treating ulcerative colitis

一种溃疡性结肠炎、抑制剂的技术，应用在医药领域，能够解决未有研究公开溃疡性结肠炎治疗的关系等问题，达到缓解溃疡性结肠炎的效果

Active Publication Date: 2021-11-26
JIANGSU PROVINCIAL HOSPITAL OF TCM +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Currently, no studies have disclosed the relationship between ASS1 or BCKDK and the treatment of ulcerative colitis

Method used

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  • Application of ASS1 or BCKDK inhibitor in preparation of medicine for treating ulcerative colitis
  • Application of ASS1 or BCKDK inhibitor in preparation of medicine for treating ulcerative colitis
  • Application of ASS1 or BCKDK inhibitor in preparation of medicine for treating ulcerative colitis

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Embodiment Construction

[0022] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0023] 1. Experimental materials and methods

[0024] 1. Grouping, modeling and administration

[0025] Materials: C57BL / 6 mice; dextran sodium sulfate (DSS); PBS buffer solution (PH 7.4);

[0026] Target gene: BCKDK-mouse (Gene ID: 12041), ASS1-mouse (Gene ID: 11898) Each gene corresponds to the target site: BCKDK (ID: 12041) shRNA sequence: 5'-CCTAGACACTCCCTACAAT-3'; ASS1( ID: 11898) shRNA sequence: 5'-GCCCAGATGTCCTTGAGAT-3'.

[0027] Vector construction (see Shanghai Jikai Gene Technology Co., Ltd.):

[0028] Adeno-associated virus vector information: pAAV-U6-shRNA-CMVbGlobin-eGFP-3Flag (GV390).

[0029] Encapsulated virus (see Shanghai Jikai Gene Technology Co., Ltd.):

[0030] Jikai Gene AAV Helper-Free System consists of viral vector (carrying target gene interf...

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Abstract

The invention discloses application of an ASS1 or BCKDK inhibitor in preparation of a medicine for treating ulcerative colitis. It is found that in the ulcerative colitis, transcriptional levels and protein levels of ASS1 and BCKDK are remarkably increased, and the ulcerative colitis can be remarkably relieved by interfering expression of a gene ASS1 or BCKDK. Therefore, the ASS1 or BCKDK can be applied to preparation of the medicine for preventing and treating the ulcerative colitis as a drug action target and can be used for screening and finding the medicine for preventing and treating the ulcerative colitis as the drug action target, the ASS1 or BCKDK inhibitor can be used for preparing the medicine for preventing and treating the ulcerative colitis, the ASS1 refers to an ASS1 gene or protein, and the BCKDK refers to a BCKDK gene or protein; and the inhibitory activity of the inhibitor means inhibiting the activity of the ASS1 or BCKDK protein, or the expression of the gene ASS1 or BCKDK.

Description

[0001] This application is a divisional application of the patent application with the application number "202010605346X" and the invention title "Application of ASS1 or BCKDK inhibitors in the preparation of drugs for the treatment of ulcerative colitis" submitted on June 29, 2020. technical field [0002] The invention belongs to the field of medicine and relates to the application of known target inhibitors in the treatment of ulcerative colitis, in particular to the application of ASS1 or BCKDK inhibitors in the preparation of medicines for the treatment of ulcerative colitis. Background technique [0003] Ulcerative colitis (UC), also known as chronic nonspecific ulcerative colitis or idiopathic ulcerative colitis, is a common chronic intestinal disease and a multifactorial and multilayered disease with unknown causes. nonspecific inflammation. The lesions are mainly in the mucosa and submucosa of the colon, showing continuous and diffuse distribution, most of which inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61P1/04C12Q1/6883G01N33/573
CPCC12Q1/6883G01N33/573A61K45/00C12Q2600/136C12Q2600/158G01N2333/912G01N2333/9015G01N2500/04A61P1/04
Inventor 刘史佳周伟陈玉根陈选青徐民民卢晓云
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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