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Simple, convenient efficient bioassay reagent and method

A technology of biological assay and test method, applied in biological testing, botanical equipment and methods, biocides, etc., can solve the problems of large difference in exposure time, cumbersome operation, incomplete exposure amount, etc., and achieve the difference in exposure time. Small, reduce economic losses, easy to operate the effect of poisoning

Active Publication Date: 2021-11-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This bioassay method is cumbersome to operate and has high requirements on the size and thickness of artificial feed, which leads to uncontrollable amount of poisoning, possibly incomplete poisoning, long time of poisoning, and large differences in indirect poisoning time of individual animals to be tested, and cannot be accurate. Disadvantages such as estimating the end time of drug exposure

Method used

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  • Simple, convenient efficient bioassay reagent and method
  • Simple, convenient efficient bioassay reagent and method
  • Simple, convenient efficient bioassay reagent and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Edible pigment has no significant effect on the growth and development of cotton bollworm

[0039] In this example, a treatment group and a control group were set up to carry out bioassay research on edible pigments.

[0040] Pick a sufficient number of cotton bollworms at the end of the third instar in advance and put them in plastic insect rearing boxes for 12 hours of starvation treatment for later use.

[0041] Treatment group: 1000 μL ddH 2 O+2μL edible coloring was prepared as a solution.

[0042] The artificial feed was cut into a size of 0.5 cm*0.5 cm*0.1 cm, and 2.5 μL of the solution was added dropwise to the piece of feed. This piece of feed is added in the plastic worm box, and fed to the cotton bollworm (4th instar 1 day) that has been starved in advance.

[0043] Control group: 1000 μL ddH 2 O.

[0044] Cut the artificial feed into a size of 0.5cm*0.5cm*0.1cm, and add 2.5μL of ddH to the piece of feed 2 O. This piece of feed is added in...

Embodiment 2

[0050] Embodiment 2 Utilizes the method of the present invention to carry out bioassay simple and efficient

[0051]In this example, the traditional bioassay method and the bioassay method of the present invention are used to compare the two methods for NaSNPV, the stomach poison of cotton bollworm.

[0052] 1. Preparation of reagents and materials

[0053] The concentration of the NasNPV suspension fed in this example is all 4*10 7 OBS / mL, the specific configuration method of the virus suspension is: use the NaSNPV stock solution with a concentration of 5 billion OBS / mL purchased by Keyun Biological Co., Ltd., and use ddH 2 O was serially diluted and configured into 1000 μL of 4*10 7 OBS / mL of virus suspension.

[0054] The specific preparation method of the bioassay reagent is as follows: adding 2 μL of edible pigment to the virus suspension to prepare.

[0055] Pick a sufficient number of cotton bollworms at the end of the third instar in advance and put them in plastic...

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Abstract

The invention relates to the field of bioassay, and particularly relates to a simple, convenient and efficient bioassay reagent and method. The bioassay reagent is a liquid containing a pesticide to be detected and an edible pigment. When a biological test is carried out, to-be-tested animals are fed with the bioassay reagent, the eating condition is observed, and when stomach toxicity of 85-95% of the to-be-tested animals is finished, the to-be-tested animals are fed with normal foodstuff, and relevant indexes are observed and counted. After the post-bioassay reagent is used, the stomach poisoning operation is simple and convenient, the efficiency is high, and the stomach poisoning efficiency is improved by about 2 times. Moreover, the stomach toxicity amount is controllable, the stomach toxicity time is short, the stomach toxicity time difference between individual to-be-tested animals is small, and the stomach toxicity ending time can be almost accurately estimated. The advantages are beneficial to promoting related research of biological pesticides and chemical pesticides, and an economic loss caused by farmland pest damage is reduced.

Description

technical field [0001] The invention relates to the field of bioassay, in particular to a simple and efficient bioassay reagent and method. Background technique [0002] Farmland pests are an important factor affecting agricultural production, and the economic losses caused by farmland pests are serious every year. As an important strategy of agricultural production, integrated pest management plays an important role in the sustainable development of agriculture and the reduction of agricultural economic losses. The use of biological pesticides and chemical pesticides to control pests has always been an important research content in the research of integrated pest management. At the same time, related research on biological pesticides and chemical pesticides usually requires relevant biological assays. [0003] Traditional bioassay experiments usually rely on the artificial feed of the animals to be tested, and the research reagents are dropped on the artificial feed to fee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A01N63/40A01P7/04
CPCG01N33/5085A01N63/40
Inventor 刘小侠蔡丽美田志强李贞刘孝明
Owner CHINA AGRI UNIV
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