Folium artemisiae argyi extract and preparation method and application thereof
A wormwood leaf extract and the technology of the extract are applied in the field of plant-derived antibacterial preparations, which can solve the problems of complex formula and low recurrence rate, and achieve the effects of expanding the scope of application, simple preparation and wide sources.
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[0036] Another typical embodiment of the present invention provides a method for preparing the above-mentioned refined extract of Folium Artemisiae Argyi, comprising the following steps:
[0037] (1) The macroporous adsorption resin on the above-mentioned Folium Artemisiae Argyi extract is washed with water, 10% (v / v) ethanol, and 20% (v / v) ethanol successively to remove polysaccharides, proteins and phenolic acids; Pore adsorption resin can be selected from D101, AB-8, ADS-4, ADS-8, SPD-100, LSA-20, XAD-5, preferably D101;
[0038] (2) sequentially use 40% to 95% ethanol aqueous solution for elution; preferably, sequentially use 40%, 60%, and 95% ethanol aqueous solution for elution, and wash until nearly colorless;
[0039] (3) Collect eluents of various concentrations, remove the eluting solvent, and obtain the refined Artemisia argyi leaf extract after freeze-drying; preferably, the eluting solvent is removed by rotary steaming under reduced pressure at 40-50°C.
[0040...
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