Supermolecule capable of being competitively combined with cell surface receptor to prevent novel coronavirus
A competitive binding, cell surface technology, applied in the supramolecular field that competes with the new coronavirus to bind cell surface receptors, can solve the complex preparation process of antibodies for the treatment of new coronavirus, increase the probability of immune system discovery, reduce intracellular replication, etc. problem, to reduce intracellular replication, inhibit early virus replication, and reduce cell infection
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Embodiment 1
[0025] The present invention designs a supramolecular nanostructure ( figure 1 ). The supramolecular nanostructure competitively blocks the binding of the new coronavirus to the cell surface NRP1 protein, preventing the new coronavirus from using the NRP1 receptor protein to enter the cell.
[0026] The nanostructure is composed of two parts: a nanogold supramolecular structure and a ligand TPP peptide segment specifically binding to NRP1 receptor protein. 1) The nano-gold supramolecular structure is used to prolong the residence time of the entire supramolecular nanostructure in the blood and lymphatic circulatory system, preventing drugs from being excreted prematurely by macrophages or other clearance mechanisms in the human body; 2) attaching to the supramolecular nanostructure The ligand TPP peptide on the surface of the structure can compete with the specific fragment of the new coronavirus S protein cleaved by furin to bind to the NRP1 receptor protein, reducing the pr...
Embodiment 2
[0028] The nano-gold supramolecular structure of the present invention utilizes the programmable complementary pairing of DNA molecules to control the biotransmission and elimination of gold nanoparticles. The basic module is constructed by modifying the DNA single strand on the surface of the gold nanoparticle, and the basic module is assembled into a supramolecular structure based on the complementary pairing of the DNA strands on it ( figure 2 in a). The size, surface chemistry and other properties of the supramolecular structure can be realized by the length of the DNA molecule modified on the surface of the basic module and whether other polymers are further modified ( figure 2 b in and figure 2 in c).
[0029] figure 2 It is a schematic diagram of nano-gold supramolecular nanostructure design of the present invention; figure 2A in the nanoparticle surface modification DNA molecule as a linker (linker), forming a basic module, the nucleotide sequence of the DNA l...
Embodiment 3
[0033] The peptide in the supramolecular in the present invention is the ligand TPP peptide that specifically binds to the NRP1 receptor protein. The amino acid sequence of the TPP peptide is shown in SEQ NO:1, SEQ NO:2, SEQ NO:3 and SEQ NO:4, specifically P1: CRGERPPR, P2: CRPARPAR, P3: CRPAKPAR or P4: CPVTRPPR. Neuropilin (NRP1) plays a role in facilitating the infection of human cells by the new coronavirus. TPP functional peptides (such as iRGD) are specific ligands of NRP1 with high affinity and specificity. The TPP functional peptide can be connected to the DNA molecule through a mild click chemical reaction, and then modified to the surface of the gold nanoparticle in the supramolecular structure through the terminal sulfhydrylated DNA molecule. The nucleotide sequence of the DNA molecule is shown in SEQ NO:6. Specifically, it is 5'N=N=N-taacaacgatccctcaaaaaaaaaa—SH-3'. Realize the covalent connection of functional peptides and supramolecular nanostructures, and reali...
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