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Bacteriostatic bacillus, liquid fermentation step thereof and bacteriostatic test method

A bacillus, liquid fermentation technology, applied in the field of new preserved strains, can solve problems that threaten animal husbandry and human health

Pending Publication Date: 2021-11-30
洛阳欧科拜克生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the widespread use of antibiotics in livestock and poultry feed, especially the excessive use, it provides enough opportunities for bacteria to evolve resistance to various drugs, which in turn leads to the emergence of drug resistance of various pathogenic bacteria, directly threatening Livestock and Human Health

Method used

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  • Bacteriostatic bacillus, liquid fermentation step thereof and bacteriostatic test method
  • Bacteriostatic bacillus, liquid fermentation step thereof and bacteriostatic test method
  • Bacteriostatic bacillus, liquid fermentation step thereof and bacteriostatic test method

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Embodiment Construction

[0041] A kind of bacteriostatic bacillus, the place where the bacteriostatic bacillus described in the present invention is stored is the General Microbiology Center of China Microbiological Strain Preservation Management Committee; the preservation time is April 6, 2021; the preservation registration number is CGMCCNo. 22124.

[0042] The reference biological material of the bacteriostatic bacillus described in the present invention is OKX101.

[0043] The liquid fermentation step of the above-mentioned bacteriostatic bacillus of the present invention comprises the following steps:

[0044] Step 1) strain activation; step 2) preparation of primary seeds; step 3) preparation of secondary seed tanks; step 4) liquid fermentation medium configuration; step 5) liquid fermentation; step 6) spray drying.

[0045] The above-mentioned liquid fermentation step of the present invention, its step is specifically,

[0046] Step 1) strain activation

[0047] Insert the glycerol tube of ...

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Abstract

The invention relates to a bacteriostatic bacillus, a liquid fermentation step thereof and a bacteriostatic test method. The preservation place of the bacteriostatic bacillus is the China General Microbiological Culture Collection Center; the preservation time is April 6, 2021; and the preservation registration number is CGMCC No. 22124. The bacteriostatic bacillus has the advantages that the bacteriostatic bacillus still has remarkable bacteriostatic performance on pathogenic bacteria such as escherichia coli and salmonella after liquid fermentation, is high in long-term placement stability, strong in acid resistance and cholate resistance, capable of being produced in an industrial scale and more beneficial to resistance substitution of microecological preparation products.

Description

technical field [0001] The invention belongs to the technical field of new preserved strains, and in particular relates to a liquid fermentation step and a bacteriostasis test method of the strains. Background technique [0002] Antibiotics are one of the greatest discoveries of the 20th century. In the past century, penicillin alone has saved about 200 million lives. While antibiotics bring benefits to humans, they also create huge economic benefits for the farming industry. In 1951, with the first approval of the US Food and Drug Administration (FDA) to add over-the-counter antibiotics to feed. Therefore, on a global scale, low-dose antibiotics as growth promoters (Antibiotics as Growth Promoter, AGP) are added to conventional feed and widely used in the animal husbandry industry. It has also witnessed the rapid development of international animal husbandry. In the past 70 years, more than 60 kinds of antibiotics have been used in animal husbandry. However, due to the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/18C12R1/07
CPCC12N1/20C12Q1/18Y02A50/30
Inventor 杨小进刘旭乐李保民黄庆周王春芳李振田李旺曾庆勋侯冰娜周鸽鸽范收武
Owner 洛阳欧科拜克生物技术股份有限公司
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