Bacillus safensis YZS-C03 and application thereof

A technology of YZS-C03 and Bacillus suffolus, which is applied in the field of microorganisms, can solve the problems of insufficient effective microbial resources and low treatment efficiency, and achieve the effect of great application prospects

Pending Publication Date: 2021-12-03
MINJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the safe disposal technology of waste polyethylene and other plastics is imminent. At present, there are few studies on the biodegradation of plastic waste such as polyethylene, and there are not many effective microbial resources and low treatment efficiency.

Method used

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  • Bacillus safensis YZS-C03 and application thereof
  • Bacillus safensis YZS-C03 and application thereof
  • Bacillus safensis YZS-C03 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Isolation, screening and identification of Bacillus shamfu

[0022] (1) Separation and screening process:

[0023] By gradient dilution method, 319 isolates were isolated from Fujian mangrove plants. Purify the isolated endophytic bacteria by the three-section line method, and judge whether the strains are purified by microscopic examination, number the purified bacteria, pick single colonies, and transfer them to NA slant medium for storage. Coat sterilized polyethylene powder 0.02g / plate (plate diameter 9cm) on the surface of the non-carbon source mineral salt solid medium plate, culture the bacterial strain obtained above by streaking on the surface, and screen the rapidly growing single colony on the NA plate cultured on culture medium. Through the growth determination of carbon-source-free mineral salt medium with polyethylene as the only carbon source, 7 strains with good polyethylene degradation effect were preliminarily screened out, and an endophyti...

Embodiment 2

[0032] Example 2 Degradation effect of Bacillus shamfu to polyethylene film

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Abstract

The invention provides bacillus safensis YZS-C03 and application thereof, and belongs to the technical field of microorganisms. The bacillus safensis YZS-C03 is classified and named as bacillus safensis YZS-C03, and is preserved in the China General Microbiological Culture Collection Center on February 1, 2021, the preservation number is CGMCC No.21798, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus safensis YZS-C03 has a very good effect in the aspect of polyethylene plastic biodegradation, provides a new resource and a solution for biodegradation of polyethylene wastes in the environment, and has great application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacillus suffolus YZS-C03 and its application in polyethylene plastic biodegradation. Background technique [0002] Plastics are an important organic synthesis Polymer material with a wide range of applications. However, plastics are difficult to degrade naturally in the environment, and the "white pollution" caused by waste plastics is becoming more and more serious. Polyethylene (PE for short) is Vinyl produced by polymerization thermoplastic resin . Polyethylene is odorless, non-toxic, feels like wax, has excellent low temperature resistance, chemical stability Good, resistant to most acid and alkali corrosion. Insoluble in normal temperature solvent , water absorption Small, electrical insulation Excellent, easy to accumulate in the natural environment, causing secondary pollution. Therefore, the safe disposal technology of waste polye...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/07
CPCB09B3/00
Inventor 袁宗胜刘芳谢宝贵
Owner MINJIANG UNIV
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