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Bergenia oxygen methyl transferase BpOMT1 gene and application thereof in preparation of 4-methoxy gallic acid

A technology of oxymethyltransferase and gallic acid, applied in the direction of transferase, application, genetic engineering, etc., can solve the problem that the synthetic route is not clear, the function of oxymethyltransferase has not been verified, and the progress of petracenin biosynthesis is affected and other problems to achieve the effect of reducing the difficulty of chemical synthesis, facilitating separation and purification, and reducing demand

Active Publication Date: 2021-12-03
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, the synthesis pathway of gallic acid to catalyze the methylation reaction of the C-4 hydroxyl group to form 4-methoxygallic acid is not clear, and the function of the oxymethyltransferase responsible for methylation has not been verified, which affects rock Advancement of Brassicin Biosynthesis Work

Method used

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  • Bergenia oxygen methyl transferase BpOMT1 gene and application thereof in preparation of 4-methoxy gallic acid
  • Bergenia oxygen methyl transferase BpOMT1 gene and application thereof in preparation of 4-methoxy gallic acid
  • Bergenia oxygen methyl transferase BpOMT1 gene and application thereof in preparation of 4-methoxy gallic acid

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Embodiment 1

[0039] Based on the basic function annotation information of the transcriptome Unigene of P. chinensis, the OMT candidate genes were screened in the sequencing annotation results. At the same time, the oxygen methyltransferase (OMT) identified in the plant was analyzed by sequence-local BLAST, and then the screening results were sorted out. Finally, an oxygen methyltransferase (OMT) gene was found. After a series of work such as cDNA preparation, candidate gene amplification and recovery, homologous recombination, protein expression, in vitro enzyme activity reaction, and HPLC and LC / MS / MS detection, it was finally identified that it can catalyze gallic acid (Gallic acid). acid) The methylation reaction on the hydroxyl group on the C-4 position generates the target candidate oxygen methyltransferase BpOMT1 gene of 4-methoxygallic acid ( figure 1 ). The steps of each stage of 4-methoxygallic acid synthesis are as follows (the following reagents, raw materials, instruments and ...

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Abstract

The invention discloses a bergenia oxygen methyl transferase BpOMT1 gene and an application thereof in preparation of 4-methoxy gallic acid. The sequence of the bergenia oxygen methyl transferase BpOMT1 gene is as shown in SEQ ID NO: 1. Gallic acid and S-adenosine-methionine are taken as raw materials, and 4-methoxy gallic acid is generated under the catalysis of bergenin oxygen methyltransferase BpOMT1, and has important significance on regulation and control research on biosynthesis of bergenin.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a gene of rock cabbage oxygen methyltransferase BpOMT1 and its application in the preparation of 4-methoxygallic acid. Background technique [0002] Rock cabbage (Bergenia purpurascens) is a plant of the genus Bergenia in the saxifrage family. There are 10 species of this genus in Asia, except for 3 species produced in East Asia, northern South Asia and southeastern Central Asia, they are mainly distributed in Shaanxi (Qinling), Xinjiang, Sichuan, Yunnan and Tibet in China. The whole plant of the genus Brutus contains baicalenin, especially the content of bacumenin extracted from the rhizome of baicalensis is as high as 8.2%. Bergenin has various effects such as antitussive, anti-inflammatory, anti-anxiety, anti-oxidation, anti-malarial, anti-cancer, treatment of diabetes, anti-hepatotoxicity, immune regulation and neuroprotection (Shihao Xiang,. BergeninExerts Hepatopro...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/70C12N1/21C12N15/11C12P7/42C12Q1/6895C12R1/19
CPCC12N9/1007C12N15/70C12P7/42C12Q1/6895
Inventor 张广辉刘祥宇陈碧环王益娜袁慧娟向贵生张敬丽郝冰
Owner YUNNAN AGRICULTURAL UNIVERSITY
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