Novel coronavirus one-step method magnetic bead nucleic acid detection kit and use method thereof

Abstract

The invention provides a novel coronavirus one-step method magnetic bead nucleic acid detection kit and a use method thereof. Two specific target gene sequences 2019 nCoV1 and 2019 nCoV2 nucleic acid sequences are extracted through one-step method magnetic bead nucleic acid and are detected, so that the precise detection of the novel coronavirus is realized; the whole detection duration does not exceed 90 min; the time is saved; and the efficiency is high. The detection kit provided by the invention can maintain high detection sensitivity and specificity; a magnetic bead method is used for performing fast nucleic acid cracking adsorption on a sample; the magnetic bead washing is not needed; the magnetic bead and an amplification reagent are directly in contact for nucleic acid amplification; the operation steps are reduced; meanwhile, the interference of impurities on the detection is avoided; the required sample quantity is small, and can be realized through 20 to 30 microliters or less; the operation is simplified; and the operation by an automatic platform can be realized.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnostic biology and relates to a one-step magnetic bead nucleic acid detection kit, in particular to a novel coronavirus one-step magnetic bead nucleic acid detection kit and a method of use thereof. Background technique [0002] Infectious diseases are one of the main causes of human death, among which respiratory tract infection is the most common. More than 90% of acute respiratory infections are caused by viruses, including respiratory syncytial virus, adenovirus, rhinovirus, coronavirus, human metapneumovirus, influenza virus, etc. High, easy to mutate antigens as the main features. Symptoms of respiratory infections are similar and often difficult to differentiate clinically. Moreover, the epidemic seasons and peaks overlap with each other, and even co-infections often exist, so rapid and accurate pathogen detection is conducive to correct treatment. In addition, since antibacterial ...

AI Technical Summary

Problems solved by technology

However, in order to obtain a good nucleic acid extraction effect and nucleic acid detection through the magnetic bead method, especially hydroxyl magnetic beads, it takes a lot of effort to optimize the reagent system for magnetic bead extraction, find a suitable nucleic acid extraction reagent system and select specific gene sequence fragments for testing

[0006] The nucleic acid extraction process of the 2019-nCoV nucleic acid detection kits currently on the market is complicated; the required sample volume is relatively high, generally at least 1mL; the overall time for nucleic acid extraction and detection is relatively long, at least 70 minutes for detection alone, plus the nucleic acid extraction stage It usually takes 3 hours; the detection sensitivity and specificity are poor

Moreover, there is no relevant report about the one-step magnetic bead nucleic acid extraction and detection kit for the new coronavirus, nor about the specific gene sequence fragments suitable for the one-step magnetic bead nucleic acid extraction and detection of the new coronavirus.

Images