Helicobacter pylori (Hp) antigen and fecal occult blood (Fit) joint detection colloidal gold test strip as well as preparation method and application of colloidal gold test strip

Abstract

The invention provides a helicobacter pylori (Hp) antigen and fecal occult blood (Fit) joint detection colloidal gold test strip. A sample pad, a gold conjugate pad, a reaction membrane and an absorption pad are sequentially formed from a sample adding end; the gold conjugate pad contains a first mouse anti-Hp monoclonal antibody Hp-Ab1 and/or a first mouse anti-hemoglobin monoclonal antibody Hb-Ab1; and a second mouse anti-Hp monoclonal antibody Hp-Ab2 and/or a second mouse anti-hemoglobin monoclonal antibody Hb-Ab2 are/is contained on the reaction membrane. According to the test strip, sample detection indexes can be accurately quantified, subjectivity of manual interpretation is avoided, conventional detection can be completed in hospitals, and self-detection can be achieved at home. Compared with a test strip for a single substance to be detected, the test strip has the advantage that multiple indexes of one sample can be detected at the same time. The Hp antigen index is suitable for auxiliary diagnosis and eradication treatment effect evaluation of helicobacter pylori infection; and the Fit index is suitable for clinical diagnosis and curative effect observation of lower digestive tract hemolytic diseases and colorectal cancer. Combined detection of the Hp antigen index and Fit index is more suitable for general screening of gastrointestinal tumors, and reduces the cancer death rate.

Description

technical field [0001] The application belongs to the field of tumor marker detection, in particular, the application relates to a colloidal gold paper strip for detecting Helicobacter pylori (Hp) antigen and human hemoglobin, its preparation method and application. Background technique [0002] According to the data of "China Health and Family Planning Statistical Yearbook 2015", among the number of deaths caused by diseases in my country, cancer has become the first factor. Especially in the urban population, the number of deaths caused by cancer far exceeds that of heart disease at 27%. There are more than 10,000 new cases of cancer every day in the country. Therefore, cancer has become an important killer affecting the health of our country. However, when the cancer incidence rate in my country is comparable to the world level, the mortality rate is higher than the world level (the cancer mortality rate in China is 17% higher than the global average level). In addition...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in this application is: the current clinical use of screening and diagnostic technology for digestive tract cancer does not have index quantification, the results are not intuitive enough, the cost is high and the detection equipment needs to be maintained later, the false positive rate of detection is high, and the repeatability of the results is not good , the operation is complicated and cannot be widely used in primary medical systems and families, the coverage of test samples is incomplete, and one sample cannot detect multiple indicators, and it is impossible to combine early diagnosis of digestive tract cancer with later observation of curative effect, so it is not suitable for clinical application , the applicability is not wide enough

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