Screening method and application of strain capable of dispelling effects of alcohol
A screening method and technology for strains, applied in the field of strain screening, can solve problems such as poor hangover effect and slow efficacy of drugs
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Embodiment 1
[0045] A screening method for strains capable of relieving alcohol, comprising the following steps:
[0046] Step 1. Sterilize the screening medium, and after the sterilization process is completed, add sterile acetaldehyde to the screening medium, then insert the natural fermentation product aseptically and carry out anaerobic culture, and then detect the acetaldehyde in the liquid phase. Aldehyde concentration, single colony plate separation of microbial flora with reduced acetaldehyde concentration and surviving bacteria;
[0047] Wherein, described aseptic acetaldehyde adopts following method to prepare:
[0048] Use a sterile filter to filter 40% acetaldehyde into a sterile container. According to the final concentration of 0.1%, 1L of screening medium contains 2.5ml sterile acetaldehyde with a volume fraction of 40%;
[0049] Wherein, the culture temperature of the sterile acetaldehyde in the screening medium is 30°C, and it is cultivated in an anaerobic environment for...
Embodiment 2 50
[0063] According to the method of Example 1, the difference is that the bacterial strains isolated and screened on the single colony plate are different, such as
[0064] Table 1 shows:
[0065] strain relative enzyme activity strain relative enzyme activity strain relative enzyme activity Example-2 1 Example-19 35 Example-36 5 Example-3 5 Example-20 21 Example-37 2 Example-4 3 Example-21 4 Example-38 22 Example-5 45 Example-22 33 Example-39 15 Example-6 12 Example-23 21 Example-40 6 Example-7 2 Example-24 15 Example-41 7 Example-8 13 Example-25 2 Example-42 46 Example-9 3 Example-26 4 Example-43 54 Example-10 23 Example-27 17 Example-44 11 Example-11 3 Example-28 21 Example-45 3 Example-12 11 Example-29 1 Example-46 17 Example-13 4 Example-30 16 Example-47 1 Example-14 5 Example-31 21 Example-48 57 Exam...
Embodiment 51 50
[0071] According to the method of embodiment one, the difference is that the initial pH value of the screening medium is different, as shown in table 3:
[0072] initial pH OD600 Final pH 3.0 1.2 2.6 4.0 2.4 3.4 5.0 4.0 4.1 6.0 7.1 4.9 7.0 10.6 5.1 8.0 7.5 5.9
[0073] It can be seen from Table 3 that when the initial pH value is 7.0, the OD600 shows the maximum absorbance value, and it can be seen that the optimum growth pH of the bacteria is 7.0.
[0074] OD600 refers to the absorbance value of a certain solution at a wavelength of 600nm, and through the definition of absorbance value, it can be seen that the absorbance value is proportional to the concentration of light-absorbing substances in the solution, that is, the concentration of the bacterial suspension is proportional to the absorbance value.
[0075] According to another aspect of the present invention, a kind of application of strain is provided, comprising th...
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