Acinetobacter strain and application thereof

A technology of bacilli and strains, applied in the field of bioengineering, can solve problems such as the inability to improve the evaluation results, and achieve the effects of reduced irritation, improved fragrance quality, and enhanced sweetness

Active Publication Date: 2021-12-07
CHINA TOBACCO ZHEJIANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Patent CN101818123B discloses a kind of Acinetobacter, which can be used to reduce the nicotine content in tobacco, so as to reduce the pollution to the environment. However, it can not increase the content of aroma substances in tobacco leaves, so it cannot finally improve the smoking evaluation results.

Method used

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  • Acinetobacter strain and application thereof
  • Acinetobacter strain and application thereof
  • Acinetobacter strain and application thereof

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Embodiment 1

[0029] This embodiment provides an Acinetobacter strain that can effectively improve the aroma quality of tobacco leaves. The Acinetobacter sp. is named Acinetobacter sp. in taxonomy. General Microbiology Center of the Management Committee, its deposit number is CGMCC NO.22157; Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip Code: 100101, Tel: 010-64807355.

[0030] The nucleotide sequence of the 16S rDNA of Acinetobacter has the sequence shown in SEQ ID NO.1.

[0031] The Acinetobacter strains are Gram-negative bacteria, such as figure 1 and figure 2 As shown, the size of the strain is (0.9-1.6) μm×(1.5-2.5) μm, it is thin rod-shaped, without flagella, and it is a white round spot on the plate.

[0032] The method for cultivating Acinetobacter includes the following steps: Inoculate Acinetobacter into the culture medium with an inoculum amount of 1% to 5% by volume, at a temperature of 28 t...

Embodiment 2

[0047] 1. Experiment preparation

[0048] Published formula of liquid LB medium:

[0049] Yeast powder 5g / L, peptone 10g / L, sodium chloride 10g / L.

[0050] Preparation of seed solution:

[0051] Inoculate the Acinetobacter sp. stored in the slant into 30 mL of LB medium, cultivate for 12 hours and set aside.

[0052] 2. Microbial treatment

[0053] Inoculate the Acinetobacter sp. strain into the liquid LB medium, and cultivate overnight on a shaker at 28-35°C and 100-150r / min;

[0054] Centrifuge the cultured bacterial solution at 4°C and 10000r / min for 10min, discard the supernatant, redissolve with the same amount of sterile water, centrifuge again in the centrifuge, discard the supernatant, and use twice as much Reconstitute with sterile water. Shake the reconstituted bacterial suspension evenly, and the amount of bacteria to be treated is 0.22×10 7 ~1.32×10 7 cfu / g Spray the suspension evenly on the surface of low-grade tobacco leaves (YN0117BEJ) of Zhejiang China T...

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Abstract

The invention belongs to the field of bioengineering, and relates to a strain, in particular to an acinetobacter strain and application thereof. The acinetobacter strain is preserved in the China General Microbiological Culture Collection Center on April 9, 2021, and the preservation number of the acinetobacter strain is NO: 22157. The strain can be used as a fermentation strain to play a role in tobacco leaf fermentation after redrying. According to low-grade tobacco leaves fermented by the acinetobacter and redried, the content of aromatic substances such as furfural, 4-cyclopentene-1, 3-diketone, benzaldehyde, benzyl alcohol, phenylacetaldehyde, 2-acetyl pyrrole, linalool, phenethyl alcohol, 4-oxoisophorone, alpha-damascenone, beta-damascenone, solanone and dihydrodamascenone in the tobacco leaves can be increased, the aroma quality and the aroma amount of the tobacco leaves are improved, the irritation is reduced, and the smoking quality of cigarettes can be obviously improved.

Description

technical field [0001] The invention belongs to the field of bioengineering and relates to a bacterial strain, in particular to an Acinetobacter strain and its application. Background technique [0002] Tobacco industry enterprises currently generally have a large number of low-grade tobacco leaves, which cannot enter the high-end products of cigarettes in production. The so-called low-grade tobacco leaves refer to a type of tobacco leaves that cannot meet the use standards of medium and high-grade tobacco leaves due to the quality defects of the tobacco leaves. The main reason for the inventory of low-grade tobacco leaves is the structural contradiction between the supply of agricultural production of tobacco leaves and the demand of cigarette industry production. For a long time, the production and utilization of low-grade tobacco leaves has been the production and utilization of low-grade tobacco. Due to the continuous improvement of the cigarette consumption structure i...

Claims

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Application Information

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IPC IPC(8): C12N1/20A24B15/20C12R1/01
CPCC12N1/20A24B15/20
Inventor 李石头帖金鑫徐清泉毕一鸣郝贤伟卫金洪赵振杰何文苗吴继忠程昌合廖付黄芳芳毛多斌郭蒙浩
Owner CHINA TOBACCO ZHEJIANG IND
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