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Application of peptides in regulating the transformation of macrophages in vitro

A technology of macrophages and cell suspensions, applied in the field of biomedicine, can solve unknown problems and achieve stable and remarkable transformation effects

Active Publication Date: 2022-01-18
渤海水产食品(山东)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, it has been found that multiple signaling pathways are related to the M1 and M2 transformation of macrophages, and there are many regulatory molecules for different signaling pathways, so that the corresponding signaling pathways are closed or activated to realize the transformation of macrophages. Regulatory molecules include various substances such as miRNA and small molecular proteins, but they have only been reported in scientific research literature, and it is unknown whether they can actually be used to induce effectiveness in vitro or not to evaluate the corresponding drug effects

Method used

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  • Application of peptides in regulating the transformation of macrophages in vitro
  • Application of peptides in regulating the transformation of macrophages in vitro
  • Application of peptides in regulating the transformation of macrophages in vitro

Examples

Experimental program
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Embodiment 1

[0059] This example provides a preparation for regulating the in vitro transformation of macrophages, including a polypeptide (HE-D) consisting of the amino acid sequence shown in SEQ ID No. 1, primers for amplifying the gene encoding iNOS, and amplifying the The primers for the α gene, the primers for amplifying the gene encoding Arg-1, the primers for amplifying the gene encoding IL-10, and the amplification primers for the control gene β-actin, the specific primer composition is shown in Table 1.

[0060] Regulate the primer composition in the in vitro transformation preparation of macrophage described in the embodiment 1 of table 1

[0061]

Embodiment 2

[0063] This example provides a preparation for regulating the in vitro transformation of macrophages. The difference from Example 1 is that the composition of the primers is shown in Table 2.

[0064] Regulate the primer composition in the in vitro transformation preparation of macrophage described in the embodiment 2 of table 2

[0065]

Embodiment 3 and 4

[0067] On the basis of the preparations for regulating macrophage transformation in vitro provided in Examples 1 and 2, this group of examples provides a kit for regulating macrophage transformation in vitro. The kits described in Examples 3 and 4 all include orifice plates , a pipette gun and a reagent solvent, and also include the preparations for regulating macrophage transformation in vitro provided in Examples 1 and 2, respectively.

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Abstract

The invention relates to the technical field of biomedicine, in particular to the application of polypeptides in regulating macrophage transformation in vitro. The polypeptide provided by the present invention composed of the amino acid sequence shown in SEQ ID No.1 is derived from leeches. The inventors have found through research that the polypeptide can efficiently promote the transformation of M1 macrophages to M2 macrophages in vitro, and the transformation The effect is remarkable and stable, and it is suitable for in vitro batch transformation. At the same time, the polypeptide composed of the amino acid sequence shown in the sequence SEQ ID No.1 can exist stably in in vitro preparations, and is suitable for preparing related regulation products for long-term transportation, storage and large batches use. The polypeptide composed of the amino acid sequence shown in the sequence SEQ ID No. 1 is used in drug efficacy evaluation or scientific research using the transformation of M1 type macrophages into M2 type macrophages as a detection index.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of polypeptides in regulating macrophage transformation in vitro. Background technique [0002] Macrophages are widely distributed in tissues and organs of the body, and play an important role in pathogen defense, inflammatory response, homeostasis maintenance and damage repair. Macrophages are a phenotypically and functionally highly heterogeneous population of immune cells that can change their polarization state to adapt to complex external conditions. Macrophages usually include two polarized types of M1 and M2 functions, among which the M1 type can promote the inflammatory response and secrete ROS, TNF-α, IL-6, MCP-1, enhance the defense ability of the body. The M2 type can reduce the inflammatory response and secrete IL-10, TGF-β, IL-1Ra after being stimulated by IL-4 and IL-13, which is beneficial to tissue repair. Therefore, the relative number of M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0786C12N15/11C12Q1/6888C12Q1/686C12Q1/04C07K7/08G01N33/68G01N33/573
CPCC12N5/0645C12Q1/6888C12Q1/686C07K7/08G01N33/68G01N33/6863G01N33/6869G01N33/573C12N2501/052C12N2501/999G01N2333/90254G01N2333/47G01N2333/5428G01N2333/525C12Q2521/107C12Q2563/107
Inventor 宋淑亮陈锚潘炳旗王珂马士玉关浩陈晓东尹宪利
Owner 渤海水产食品(山东)有限公司
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