Lung cancer micro-tumor cell model culture method

A technology for lung cancer and tumors, which is applied in the direction of tumor/cancer cells, cell dissociation methods, and cell culture active agents, etc. The effects of rich types, short culture period and high culture stability

Pending Publication Date: 2021-12-07
SUZHOU GENOARRAY
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very important to develop a tumor model that can accurately reflect the characteristics of the patient's original lesion. However, the existing primary tumor cell culture technologies mainly include 2D culture, 3D culture, reprogramming culture, etc., and these methods are different to varying degrees. Facing problems such as extremely long culture cycle, low culture success rate, difficult removal of miscellaneous cells, and inability to reproduce the tumor microenvironment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lung cancer micro-tumor cell model culture method
  • Lung cancer micro-tumor cell model culture method
  • Lung cancer micro-tumor cell model culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1, preparation is used for cultivating the reagent of lung cancer micro-tumor model

[0085] 1. Sample preservation solution (100mL)

[0086] The specific formulation of the sample preservation solution (100mL) is shown in Table 1.

[0087] Table 1. Sample Preservation Solution (100mL)

[0088]

[0089]

[0090] After the sample preservation solution is prepared, it is divided into 15mL centrifuge tubes, 5mL per tube. After aliquoting, it can be stored at 4°C for 1 month.

[0091] 2. Sample cleaning solution (100mL)

[0092] The specific formula of the sample cleaning solution (100mL) is shown in Table 2.

[0093] Table 2. Sample cleaning solution (100mL)

[0094]

[0095] The sample cleaning solution should be prepared and used immediately.

[0096] 3. Sample dissociation solution (10mL)

[0097] The specific formulation of the sample dissociation solution (10mL) is shown in Table 3.

[0098] Table 3. Sample dissociation solution (10mL)

[...

Embodiment 2

[0162] Embodiment 2, acquisition of postoperative specimen of lung cancer

[0163] 1. Cooperate with tertiary hospitals to obtain samples through the form of clinical research initiated by researchers, and the cooperation has passed the formal medical ethics review.

[0164] 2. The attending doctor selects the patients according to the clinical indications stipulated in the medical guidelines, and selects appropriate samples for in vitro culture according to the clinical indications during the operation. The selection criteria for the samples are: primary lung cancer, pathological stage II or Stage III, the pathological type is non-small cell lung cancer or small cell lung cancer, and the weight of the lung cancer sample exceeds 20mg.

[0165] 3. All enrolled cases are uniformly coded by the date of sample collection + the last four digits of the patient’s hospital number. For example, for a sample provided on January 1, 2020, the patient’s hospital number is T001537474, and t...

Embodiment 3

[0168] Embodiment 3, pre-dissociation treatment of lung cancer tissue samples

[0169] The following operations need to be performed on ice, and the entire operation steps need to be completed within 10 minutes.

[0170] The surgical equipment used in the following operations must be sterilized by high-temperature steam (120°C, 20 minutes) in advance and dried before use.

[0171] 1. After the sample is weighed, clean the surface of the sample with medical alcohol (75% by volume) for 10 to 30 seconds.

[0172] 2. Wash the sample 5 times with sample cleaning solution, and wash the sample 5 times with sterile PBS solution.

[0173] 3. Use ophthalmic scissors, ophthalmic forceps, scalpel and other equipment to carefully peel off the adipose tissue, connective tissue, and necrotic tissue in the sample.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
diameteraaaaaaaaaa
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a lung cancer micro-tumor cell model culture method. The invention provides a novel lung cancer micro-tumor model culture technology and matched reagents, and the core of the technology is as follows: (1) using a mild cell dissociation reagent for treating lung cancer solid tumor tissues, so as to ensure the vitality of various types of cells in the tissues to the greatest extent; and (2) preparing a special serum-free culture medium, and utilizing a suspension culture system to enable various types of cells separated from lung cancer tissues to be self-assembled to form a cell mass structure with various cell components, namely a lung cancer micro-tumor model. The lung cancer micro-tumor model obtained by the method can accurately reflect various characteristics of the original focus of a patient, and is a good scientific research experiment model and a preclinical experiment model in the field of precise tumor diagnosis and treatment. It can be foreseen that the culture method has wide application prospects in the fields of lung cancer research and clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for cultivating a lung cancer micro-tumor cell model. Background technique [0002] Lung cancer is the cancer with the highest morbidity and mortality in the world. According to the WHO GLOBOCAN global cancer statistics report, the number of new cases of lung cancer in the world reached 2.09 million in 2018, accounting for 11.6% of the total number of new cancer cases; 1.76 million, accounting for 18.4% of the total number of cancer deaths. A large number of epidemiological studies have shown that the incidence and mortality of lung cancer in my country are increasing year by year due to factors such as tobacco prevalence, air pollution, and aging. It is estimated that in the next few decades, lung cancer will be the top priority of cancer prevention and treatment in my country. [0003] The treatment methods for lung cancer mainly include surgery, chemotherapy, targeted t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09A01N1/02
CPCC12N5/0688C12N5/0693A01N1/0226C12N2501/11C12N2501/115C12N2501/2302C12N2501/2315C12N2501/70C12N2500/32C12N2500/05C12N2501/18C12N2500/60C12N2501/727C12N2509/00
Inventor 席建忠尹申意李娟
Owner SUZHOU GENOARRAY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap