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Use of cyanidin-3-O-glucoside and/or protocatechuic acid in improving heat tolerance of testis

A technology of glucoside and cyanidin, which is applied in the field of biomedicine to reduce testicular damage, improve seminiferous tubule atrophy, and increase heat tolerance

Pending Publication Date: 2021-12-10
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the above-mentioned inventions are mainly aimed at the toxicity induced by chemical factors, but have not studied the damage directly caused by physical factors

Method used

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  • Use of cyanidin-3-O-glucoside and/or protocatechuic acid in improving heat tolerance of testis
  • Use of cyanidin-3-O-glucoside and/or protocatechuic acid in improving heat tolerance of testis
  • Use of cyanidin-3-O-glucoside and/or protocatechuic acid in improving heat tolerance of testis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 The protective effect of cyanidin-3-O-glucoside and / or its metabolite protocatechuic acid on heat stress-induced male reproductive damage

[0047] 1. Experimental method

[0048] 1. Grouping and handling of experimental animals

[0049] CD- (ICR) Male mice were fed adaptively, and 250 mice were randomly divided into groups using the weight stratification method: a total of 5 time points (0day, 2day, 7day, 14day and 21day), 5 groups at each time point; a total of 25 Group. Five groups of mice at the same time point were divided into Control group, HS group (100g / kg·bw), C3G+HS group (100g / kg·bw), PCA+HS group (100g / kg·bw) according to different interventions. ·bw) and Vit C+HS group (100g / kg·bw). Among them, there were 7 rats in each group in the 0day and 2day groups, and 12 rats in each group at other time points.

[0050] The specific operation is as follows:

[0051] (1) All groups of mice at all six time points were pre-intervened 7 days before model...

Embodiment 2

[0072] Example 2 Cyaniidin-3-O-glucoside and / or its metabolite protocatechuic acid can increase testicular heat tolerance

[0073] 1. Experimental method

[0074] 1. Grouping and handling of experimental animals

[0075] With embodiment 1.

[0076] 2. Protein extraction and immunoblotting

[0077] Tissue protein extraction:

[0078] (1) Accurately weigh the testicular tissue, add the lysate (RIPA:cocktail:PMSF=100:1:1) according to the ratio of 1:9 between the tissue and the lysate, add two magnetic beads, and grind it in a tissue grinder at 60Hz , Homogenize for 60s until no visible solids are visible (the module is pre-cooled before homogenization, and the lid is trimmed and tightly pressed during homogenization).

[0079] (2) Centrifuge the homogenized sample at 4° C. and 14000 g for 10 min, and take the supernatant.

[0080] (3) Repeat the step of (2) to obtain the supernatant.

[0081] (4) Determination of the protein concentration of the supernatant using a BCA kit...

Embodiment 3

[0126] Example 3 Cyaniidin-3-O-glucoside and / or its metabolite protocatechuic acid relieve oxidative stress and endoplasmic reticulum stress-mediated cell apoptosis.

[0127] 1. Experimental method:

[0128] 1. Grouping and handling of experimental animals

[0129] With embodiment 1.

[0130] 2. Determination of MDA content

[0131] (1) Preparation of sample supernatant: Accurately weigh and record the weight of testicular tissue, add 9.0 μL of pH 7.4 PBS solution for every 1.0 mg of testicular tissue, add two magnetic beads, place in a tissue grinder at 60HZ, 60s Conditions Low-temperature grinding, centrifugation for 10 minutes (4°C, 14000g), and the supernatant was taken for subsequent index determination.

[0132] (2) Preparation of main reagents:

[0133] a. Preparation of TBA stock solution: Accurately weigh 25 mg of TBA, use 6.76 mL of TBA preparation solution to form a TBA stock solution with a concentration of 0.37%, heat to 70°C and vigorously Vortex to promote d...

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Abstract

The invention discloses use of cyanidin-3-O-glucoside (C3G) and / or protocatechuic acid (PCA) in improving heat tolerance of testis. The cyanidin-3-O-glucoside and / or protocatechuic acid can relieve phenomena of atrophy of testis seminiferous tubules, vacuolation of spermatogenic epithelium, reduction of diameter and thickness of testis lumens and the like. The C3G and / or the metabolite PCA thereof can reduce a phosphorylation level of eIF2alpha and an expression of stress particles by reducing an external stress pressure suffered by the testis so as to increase heat tolerance of testis, improve an antioxidant system of the testis and regulate an IRE1alpha-XBP1 pathway so as to relieve oxidative stress and endoplasmic reticulum stress-mediated apoptosis and relieve spermatogenesis disorder and testis injury induced by heat stress. The C3G and / or the metabolite PCA thereof provides a reliable theoretical basis and hasimportant significance for relieving a male reproductive injury induced by the testis in a heat stress state due to bad living habits, occupational factors and pathological reasons in the future.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of cyanidin-3-O-glucoside and / or protocatechuic acid in improving testicular heat tolerance. Background technique [0002] Heat stress (HS) refers to the stress response produced by the body under adverse conditions such as high temperature exposure. Existing research data show that different parts of the body have different degrees of sensitivity to temperature, among which the testis is extremely sensitive to changes in temperature. Generally speaking, the testis is located in the scrotum as the site of spermatogenesis, and the temperature of the scrotum is 2-8°C lower than the core body temperature to maintain the optimal microenvironment for spermatogenesis. Elevated testicular temperature can cause spermatogenesis disorders, thereby reducing male fertility or even infertility. In daily life, unhealthy living habits such as sauna, occupational exposure, ...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61K31/192A61P39/06A61P39/00A61P15/08
CPCA61K31/7048A61K31/192A61P39/06A61P39/00A61P15/08
Inventor 白卫滨蔡冬宝蒋鑫炜李旭升孙建霞
Owner JINAN UNIVERSITY
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