High-temperature fermentation regulation and control method of erythritol

Abstract

The invention belongs to the technical field of fermentation engineering, and particularly relates to a high-temperature fermentation regulation and control method of erythritol. The method comprises the following steps of: (1) activating and culturing strains into a domesticated single plant; (2) inoculating the domesticated single plant in the step (1) into a first-stage seed culture medium, and carrying out seed amplification culture to a logarithmic phase to obtain a first-stage seed solution; (3) inoculating the first-stage seed solution obtained in the step (2) into a second-stage seed culture medium according to a proportion of 0.01-0.1% (V / V), and carrying out amplification culture to the logarithmic phase to obtain a second-stage seed solution; and (4) transferring the second-stage seed solution obtained in the step (3) into a sterilized fermentation culture medium according to the proportion of 10% (V / V,), and carrying out aerobic fermentation to obtain the erythritol. According to the method, temperature adaptive domestication culture needs to be carried out on strains from strain domestication, seed amplification culture and seed fermentation, human intervention is carried out on a thallus growth environment, the temperature in a fermentation process is increased in a stepped manner, thalli are effectively stimulated, the product amount is increased, the yield of the erythritol is increased, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for regulating and controlling high-temperature fermentation of erythritol. Background technique [0002] Erythritol is a four-carbon polyol commonly used as a low-calorie sweetener in food production. As a GRAS bacteria identified by the Food and Drug Administration, Candida lipolytica is the preferred strain for producing erythritol. Since the bacterium is aerobic fermentation, it produces more heat, but the fermentation temperature generally does not exceed 30°C. Low-temperature fermentation is conducive to the improvement of the vitality of the bacterium, strong ability to produce sugar alcohol, and high tank yield. However, in the summer in the south and north, it is difficult to cool down and the cost of power is high. Fermentation cooling has become an important factor for high production costs. Under the existing fermentation condit...

AI Technical Summary

Problems solved by technology

However, in the summer in the south and north, it is difficult to cool down and the cost of power is high. Fermentation cooling has become an important factor for high production costs.

Under the existing fermentation conditions, the erythritol cells have poor adaptability to the external temperature, and the cell activation ability is poor, so that the yield of erythritol is low, and the industrial production cost is high