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Preparation method of pretreatment reagent, pretreatment reagent and pretreatment method

A technology for reagents and protein denaturants, applied in the field of medical testing, can solve the problems of cumbersome operation steps, time-consuming pretreatment process, and impact on test accuracy, etc.

Pending Publication Date: 2021-12-10
深圳市希莱恒医用电子有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, it takes more than 90 minutes for whole blood samples to be hemolyzed by adding pretreatment reagents, which makes the pretreatment process time-consuming and cumbersome. In addition, folic acid is a photosensitive substance that is exposed to light for a long time, which affects the accuracy of the test.

Method used

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  • Preparation method of pretreatment reagent, pretreatment reagent and pretreatment method
  • Preparation method of pretreatment reagent, pretreatment reagent and pretreatment method
  • Preparation method of pretreatment reagent, pretreatment reagent and pretreatment method

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preparation example Construction

[0034] figure 1 Schematic flow chart of the present application a method for preparing the pretreatment agent according to an embodiment, see figure 1 The pretreatment agent for detecting folate in erythrocytes, the method comprising the steps of:

[0035] S10: The buffer, ascorbic acid, glutamine and GGT protein denaturing agent to form a mixture, and adjusting the pH of the mixture to a predetermined pH range.

[0036] In this embodiment, each buffer weighed, ascorbic acid, glutamine and GGT protein denaturant (for each component), the respective components were mixed to obtain a mixture. PH meter or the like can be used to test the pH of the mixed solution, and the pH of the mixture is adjusted to a predetermined pH range. Wherein the predetermined pH range within the pH range required at the time of a whole blood sample erythrocytes before the treatment. In some embodiments, the predetermined pH range is 4-5, to provide an acidic environment needed to pre-treatment. It is und...

Embodiment 1

[0060] According to the following components to prepare a mixture of 100mL using 2mL brown centrifuge tube, taken as a mixture of 0.2mL lysis buffer per tube 0.2mL aliquots lyophilized lysate, 2-8 ℃ stored sealed after lyophilization is completed .

[0061] Mixture components: sodium citrate buffer 0.5mol / L, pH = 4.5, ascorbic acid 10mg / mL, amide GGT 20μg / mL, guanidine hydrochloride 20mol / L.

Embodiment 2

[0063] According to the following components to prepare a mixture of 100mL using 2mL black tube, taken as a mixture of 0.2mL lysis buffer per tube 0.2mL aliquots lyophilized lysate, 2-8 ℃ stored sealed after lyophilization is completed .

[0064] Mixture components: sodium citrate buffer 0.5mol / L, pH = 4, ascorbic acid 50mg / mL, amide GGT 10μg / mL, guanidine hydrochloride 10mol / L.

[0065] The following comparative tests with reference to specific experiments, the performance of the pretreatment agent obtained in Preparation Example 1 of the present embodiment will be described application.

[0066] In the pretreatment agent prepared in Example 1 to give a reagent for assessing embodiment # 1, Beckman Coulter (USA), Ltd. red cell folate produced lysing agent as a control reagent # 1, comparative tests, i.e. using both Reagents for two whole blood samples were pretreated assay kit (chemiluminescence) post-treatment using Beckman Coulter (USA), Ltd. of folic acid produced afte...

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Abstract

The embodiment of the invention relates to the technical field of medical detection, and discloses a preparation method of a pretreatment reagent, the pretreatment reagent and a pretreatment method. The method comprises the steps of preparing a mixed solution, and adjusting the pH value of the mixed solution to a preset pH range; and taking a first preset quantity of the mixed solution as a lysis solution, sub-packaging the lysis solution in a centrifugal tube with a light shielding function, freeze-drying the centrifugal tube filled with the lysis solution so as to enable the lysis solution to form freeze-dried powder, and sealing and storing the freeze-dried powder to prepare the pretreatment reagent. The mixed solution comprises the following components: 0.2-1 mol / L of a buffer solution, 5-75 mg / mL of ascorbic acid, 5-50 [mu]g / mL of glutamine transpeptidase and 5-30 mol / L of a protein denaturant. Therefore, the freeze-dried powder obtained by freeze-drying the lysate comprising the components has good hemolysis treatment capacity, and the freeze-drying treatment time is short, namely the required pretreatment time is short. In addition, the lysate is stored in a centrifugal tube with a light shielding function in the form of freeze-dried powder, so that folic acid in a whole blood sample can be prevented from being exposed in light as far as possible.

Description

Technical field [0001] Example embodiments of the present application relates to medical testing technology, and in particular the former production method relates to a treatment agent, pretreatment reagents and pretreatment method. Background technique [0002] Folic acid is an important water-soluble vitamin B group, which has an important role in protein synthesis and cell division and growth, can promote the formation of normal red blood cells. Folic acid deficiency and the occurrence of many diseases, accurate detection of the human body is the basis for assessing the level of folic acid folate nutritional status. Folate levels commonly used in human plasma folate, serum folate and RBC folate to measure. Plasma or serum folate folate levels representative of in vivo circulation state, but easily affected by factors such as diet. Plasma or serum folate measurements can provide early indicators of folate status. RBC folate and red blood cell renewal process related to long-ter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/76
CPCG01N1/28G01N21/76
Inventor 王小龙徐辉陆建斌王建东
Owner 深圳市希莱恒医用电子有限公司
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