A kind of melanin separation and extraction method based on ionic liquid

A technology of ionic liquid and extraction method, which is applied in the field of waste hair recycling, can solve the problems of long pretreatment time, expensive protease, complicated operation, etc., and achieve the effect of easy separation

Active Publication Date: 2022-08-02
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The invention solves the technical problems of low melanin recovery yield, complex operation, expensive protease and long pretreatment time in the prior art.

Method used

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  • A kind of melanin separation and extraction method based on ionic liquid
  • A kind of melanin separation and extraction method based on ionic liquid
  • A kind of melanin separation and extraction method based on ionic liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0030] figure 1 It is the technical process flow of the targeted extraction of melanin based on the ionic liquid of the present invention.

[0031] Step 1: A mixture of 0.2 g of washed waste human hair (hair) and 4 g of 1-butyl-3-methylimidazolium chloride (ionic liquid) was heated in a beaker at 130° C. until the waste human hair was completely dissolved. 0.2 mL of dilute sulfuric acid (50 wt%) (depolymerizing agent) was added to the mixture, and the mixture was heated at 120° C. for 1.2 hours.

[0032] Step 2: Add 20mL aqueous solution to the mixture, shake the mixture vigorously for 2min, then use a centrifuge for centrifugation (5000rpm, 5min), obtain a melanin product after centrifugation, the yield is 6.02%, then add 20ml 0.7 g / ml K 3 PO 4 (Phase separation agent) The upper phase and the lower phase are separated. The upper layer solution is rich in 1-butyl-3-methylimidazolium chloride, is collected, and is directly recycled to step 1 after 5 hours of vacuum distilla...

Embodiment 2

[0035] Step 1: A mixture of 1 g of washed waste brown wool (hair) and 20 g of 1-butyl-3-methylimidazolium chloride (ionic liquid) was heated in a beaker at 130° C. until the waste human hair was completely dissolved. 1 mL of dilute sulfuric acid (50 wt%) (depolymerizing agent) was added to the mixture, and the mixture was heated at 120°C for 2.2 hours.

[0036] Step 2: Add 100 mL of aqueous solution to the mixture, shake the mixture vigorously for 6 min, and then use a centrifuge for centrifugation (5000 rpm, 10 min) to obtain a melanin product after centrifugation, with a yield of 7.24%, and then add 100 ml of 0.7 g / ml K 3 PO 4 (Phase separation agent) The upper phase and the lower phase are separated. The upper layer solution is rich in 1-butyl-3-methylimidazolium chloride, is collected, and is directly recycled to step 1 after 5 hours of vacuum distillation treatment. The lower layer solution is rich in complex amino acids and a phase separation agent, and the lower phase ...

Embodiment 3

[0039] SEM test was carried out on the basis of Example 1, and the test results were shown in figure 2 .

[0040] Test description: It is usually possible to directly observe whether the surface morphology of melanin maintains the original nanoparticle shape through SEM test, so as to determine whether the natural morphology and structure of melanin is destroyed. Granular melanin that maintains its natural appearance is generally considered to be an excellent melanin product.

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Abstract

The invention relates to a method for separating and extracting melanin based on ionic liquid, and belongs to the technical field of waste hair recycling. The invention proposes to use ionic liquid and depolymerization agent to depolymerize hair, then use a two-phase system to separate amino acids, ionic liquids and melanin in one step, and then extract the amino acids in the phase separation agent. The phase agent is recycled. The actual operation steps are simple, high-purity melanin can be efficiently separated, and the melanin particles remain original, which is convenient for subsequent high-end applications; the main reagents can be recovered and reused, which can greatly reduce production costs. The invention has the advantages of high efficiency, no discharge of three wastes, excellent economic feasibility and the like.

Description

technical field [0001] The invention belongs to the technical field of waste hair recycling, and more particularly relates to a method for separating and extracting melanin based on ionic liquid. Background technique [0002] Waste hair is a solid waste produced by humans and animals, with an annual global output of more than one million tons. Waste hair contains a lot of valuable constituents such as keratin and melanin. Keratin is easily hydrolyzed to produce amino acids, which can be used in many industries. Melanin is also considered to be a useful biological pigment and has been applied in such disciplines as surface modification, cell interface, biosensing applications, and nanomedicine. However, there are still many shortcomings in today's hair resource recovery process: in the industrialized process method, the recovery of amino acids is usually carried out by using strong acid hydrolysis, which is to use strong acid to hydrolyze hair under high temperature conditi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 陈朱琦罗放赵钦地姜旺王训
Owner HUAZHONG UNIV OF SCI & TECH
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