Rotating wheel type optical assembly and rotating wheel type PCR optical system

Abstract

The invention relates to a rotating wheel type optical assembly and a rotating wheel type PCR optical system. An optical channel rotating wheel is provided with an exciting light module and a fluorescence detection module, and an optical fiber wheel is provided with a collimation module and a fluorescence collection module; the exciting light module and the collimation module jointly form an excitation light path, and the fluorescence collection module and the fluorescence detection module jointly form a detection light path; and the motor is used for only rotating the optical channel rotating wheel to switch and align the excitation light path and the detection light path. The integration level of the optical system is remarkably improved, the space occupied by the structure of the optical system is reduced, fluorescence detection of multiple wavebands in a PCR reaction chamber can be realized, and the optical system is suitable for various PCR amplification systems; the switching of the optical channels is completed by the independent rotation of the optical channel rotating wheel, so that the alignment difficulty is greatly reduced, and the mechanical damage of the system is reduced; the complexity of an optical system is effectively reduced, and the use of a plurality of repeated optical channels is eliminated; and the adopted optical fiber does not have mechanical motion, and the problem that the service life is affected by optical fiber abrasion is solved.

Description

technical field [0001] The present application relates to the field of fluorescent quantitative PCR detection, in particular to a rotary optical assembly and a rotary PCR optical system. Background technique [0002] Polymerase Chain Reaction (PCR) technology is a technology that achieves a large amount of amplification of specific DNA fragments in vitro in a short period of time and then conducts qualitative analysis. However, the initial PCR instrument can only perform qualitative analysis on the final result of the amplification, and cannot monitor the amplification of each cycle of the PCR amplification reaction in real time, resulting in the unobservable reproducibility and the inability to confirm operational errors during the process, which leads to waste Experiment time. After that, real-time fluorescent quantitative PCR (Real-time qPCR) technology appeared, which added specific DNA fluorescent probes to the PCR reaction system, and monitored the amplification of th...

AI Technical Summary

Problems solved by technology

As a result, real-time quantitative PCR instruments inevitably have multiple complex optical systems, multiple channel interfaces make the structure more complex, high material costs, and low detection sensitivity.

Furthermore, the current method for switching between different optical channels is usually the double filter wheel switching method, that is, the double filter wheel switching method. This method of using two motorized wheels in the optical path inevitably has high mechanical damage and poor positioning control. Difficulty and other issues

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