Micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillatory flow effects

A technology of biological particles and separation devices, applied to laboratory containers, chemical instruments and methods, laboratory utensils, etc., can solve the problems of cost increase, long sound field action time, long flow channel, etc., and achieve simple structure and easy operation Simple, highly integrated effects

Pending Publication Date: 2021-12-17
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, like other active methods, acoustophoretic manipulation also often requires a longer sound field action time, which means that a longer flow channel is required, resulting in increased costs; of course, the sound field intensity is usually increased to shorten the action time, but Under the action of high-intensity sound field, cell viability is impaired and mechanobiological effects are unavoidable, which limits the practical application of acoustophoretic manipulation to a certain extent.

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  • Micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillatory flow effects
  • Micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillatory flow effects
  • Micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillatory flow effects

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Embodiment Construction

[0038] The technical solutions of the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.

[0039] figure 1 Shown is a schematic diagram of the structure of a micro-nano biological particle enrichment and separation device based on acoustophoresis and oscillating flow effects in the present invention. The micro-nano biological particle enrichment and separation device includes a microfluidic channel 2, an acoustic wave control system , valveless pump membrane control system, liquid storage tank, catheter;

[0040] Such as figure 2 As shown, the microfluidic channel 2 is located in the microfluidic chip, including a microfluidic channel inlet 2-1, an expanding conical tube 2-2, a constricting conical tube 2-3, a straight channel, an enrichment Sample outlet 2-4 and separated sample outlet 2-5; the height of the expansion cone 2-2 is higher than that of other channels;

[0041] Such as image 3 As shown, the ...

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Abstract

The invention relates to a micro-nano biological particle enrichment and separation device based on the acoustophoresis and oscillatory flow effects is ingenious in design, easy to operate, efficient and biologically friendly. A valveless micropump is used for generating oscillatory flow, the design of the oscillatory flow realizes infinite length of a short channel, and acoustic radiation force and inertial lift force are used for driving particles to a narrow enough micro-strip so as to realize enrichment and separation. Compared with single acoustophoresis control, the design has the advantages that the flux is higher, only a shorter straight channel is needed, the sound field intensity is low, and the operation is mild; compared with single inertial control, the design can maintain a lower level of shear stress, can reduce the risk of state change and activity loss of biological particles to a greater extent, and avoids the high-strength manufacturing requirement of a micro-fluidic chip at the same time. The device is simple in structure and high in integration level, can be used for efficient enrichment and separation of rare micro-nano biological particles such as circulating tumor cells and exosomes, and provides a platform for basic medical research and clinical rapid detection application.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and in particular relates to a microfluidic device for enriching and separating micro-nano biological particles by utilizing sound field and oscillating flow effects. Background technique [0002] The control and treatment of micro-nano biological particles are commonly used in medical testing, biochemical reactions, environmental purification and other applications. The enrichment and separation of micro-nano biological particles is a key step, and has important applications in the fields of biology, medicine, chemistry and the environment significance. In recent years, since the control scale of microfluidic technology can match that of micro-nano particles, the development of many particle enrichment and separation technologies in microfluidics has been promoted. However, the existing methods are still difficult to achieve precise and efficient manipulation of micro-nano biological...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
CPCB01L3/502753B01L3/502707B01L2300/12
Inventor 薛春东曲恒超赵家铭覃开蓉
Owner DALIAN UNIV OF TECH
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