Preparation method of transferrin, transferrin-containing composition and application

A technology for transferrin and a composition is applied in the field of preparation of transferrin, which can solve the problems of complex plasma composition, and achieve the effects of improving treatment effect, improving self-care ability and enhancing memory.

Pending Publication Date: 2021-12-17
江苏豪思睦可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the composition of plasma is extremely complex, including proteins, lipids, inorganic salts, sugars, amino acids, metabolic wastes, and a large amount of water. So far, there is no research to determine which components in plasma have the effect of improving memory, improving and treating senile dementia; and currently China's blood product companies urgently need to improve the development and utilization of plasmapheresis

Method used

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  • Preparation method of transferrin, transferrin-containing composition and application
  • Preparation method of transferrin, transferrin-containing composition and application
  • Preparation method of transferrin, transferrin-containing composition and application

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preparation example Construction

[0067] The specific preparation method of the above-mentioned transferrin is as follows: the following steps are sequentially included: preparation of pretreatment solution, purification by hydrophobic chromatography, concentration and replacement.

[0068] Step 1. Preparation of pretreatment solution: the purpose of this step is to redissolve the protein in the precipitated human plasma Cohn IV fraction, remove the filter aid, and remove some miscellaneous proteins after reconstitution.

[0069] Sub-step 1, the first reconstitution treatment: dissolve the solid Cohn IV component into PBS buffer, mix well, and obtain the first reconstitution solution.

[0070] The mass volume ratio (w / v) of above-mentioned human plasma Cohn IV component and PBS buffer is 1: (2-10), preferably 1: 3.5; Above-mentioned PBS buffer is: 10-50mmol / L PBS, pH6. 0-8.0, preferably 20mmol / L PBS, pH7.5.

[0071] Sub-step 2, the first centrifugation treatment: centrifuge the above-mentioned first complex s...

Embodiment 1

[0098] The present embodiment is the preparation method of transferrin, comprising the following steps:

[0099] S1: Preparation of human plasma Cohn IV fraction pretreatment solution:

[0100] (1) The first reconstitution: Dissolve the waste material from the production of blood products by the Nitschmann-Kistler method—the human plasma Cohn IV component solid into 20mmol / L PBS solution (pH7.5), according to 1:3.5 (w / v) After dissolving, use a magnetic stirrer to mix evenly to obtain the first complex solution.

[0101] (2) The first centrifugation: centrifuge the first complex solution at 10,000 rpm at 4° C. to obtain the first supernatant.

[0102] (3) Filtration for the first time: filter the supernatant for the first time with a 0.45 μm filter membrane to obtain the filtrate for the first time.

[0103] (4) pH adjustment: adjust the pH value of the first filtrate to 7.0 with 1 mol / L sodium citrate.

[0104] (5) Remove impurity protein: Add 15% (w / v) PEG4000 to the fir...

Embodiment 2

[0123] In this example, the sterilized transferrin (TF solution) obtained in step S4 of Example 1 is used in combination with human serum albumin (HSA) and human immunoglobulin (IgG).

[0124] The sterilized transferrin was mixed with human serum albumin and human immunoglobulin to obtain a TF+HSA+IgG mixed solution, and the mass ratio of TF, HSA and IgG in the mixed solution was 1:8.33:8.33.

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Abstract

The invention belongs to the field of application of biological medicines, and particularly relates to a preparation method of transferrin, a transferrin-containing composition and application. Preparation raw materials of the transferrin comprise a human plasma CohnIV component, and the preparation method of the transferrin comprises steps of pretreatment liquid preparation, hydrophobic chromatographic purification and concentration replacement. The transferrin disclosed by the invention can be independently used or combined with other plasma proteins, thereby playing a role of enhancing memory and improving cognition.

Description

[0001] This application is a divisional application with an application date of May 23, 2019, an application number of 201910432249.2, and an invention title of "Application of Transferrin and Composition Containing Transferrin". technical field [0002] The invention belongs to the application field of biological medicines, and in particular relates to a preparation method of transferrin, a composition containing transferrin and its application. Background technique [0003] Alzheimer's disease (Alzheimer's disease, AD), also known as senile dementia, is a degenerative disease of the brain and central nervous system caused by the loss of neurons. . In 1906, the German neuropathologist Alzheimer first reported a 51-year-old female patient with progressive memory loss. In 1910, psychiatrist Emil Kraepelin, known for naming and categorizing brain disorders, proposed the name Alzheimer's disease. The common clinical manifestations of the disease are progressive memory and cog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/79C07K1/14C07K1/34C07K1/30C07K1/20C07K1/36A61K38/40A61K38/38A61K39/395A61K31/27A61K31/13A61K31/445A61K31/55A61P25/28
CPCA61K38/40A61K38/385A61K31/445A61K31/55A61K31/27A61K31/13A61P25/28C07K14/79A61K39/395A61K2300/00
Inventor 栗琳崔雅轩谢蓝张丽丽娄钰霞
Owner 江苏豪思睦可生物科技有限公司
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