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Application of union of covelline albumen and transferrin

A technology of ceruloplasmin and transferrin, applied in the direction of transferrin, peptide/protein components, animal/human protein, etc., to achieve the effect of improving cognitive function, learning and memory ability, and self-care ability

Active Publication Date: 2019-11-15
北京豪思生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, in the current scientific research and practice, it is necessary to find out the components in plasma that can improve learning and memory ability and treat senile dementia; and there is no report on the combination of ceruloplasmin and transferrin for the above purposes

Method used

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  • Application of union of covelline albumen and transferrin
  • Application of union of covelline albumen and transferrin
  • Application of union of covelline albumen and transferrin

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preparation example Construction

[0039] The specific preparation method of the above-mentioned transferrin is as follows: the following steps are sequentially included: preparation of pretreatment solution, purification by hydrophobic chromatography, concentration and replacement.

[0040] Step 1. Preparation of pretreatment solution: the purpose of this step is to redissolve the protein in the precipitated human plasma Cohn IV fraction, remove the filter aid, and remove some miscellaneous proteins after reconstitution.

[0041] Sub-step 1, the first reconstitution treatment: dissolve the solid Cohn IV component into PBS buffer, mix well, and obtain the first reconstitution solution.

[0042] The mass volume ratio (w / v) of above-mentioned human plasma Cohn IV component and PBS buffer is 1: (2-10), preferably 1: 3.5; Above-mentioned PBS buffer is: 10-50mmol / L PBS, pH6. 0-8.0, preferably 20mmol / L PBS, pH7.5.

[0043] Sub-step 2, the first centrifugation treatment: centrifuge the above-mentioned first complex s...

Embodiment 1

[0071] The present embodiment is the preparation method of transferrin, comprising the following steps:

[0072] S1: Preparation of human plasma Cohn IV fraction pretreatment solution:

[0073] (1) The first reconstitution: Dissolve the waste material from the production of blood products according to the Nitschmann-Kistler method—the human plasma Cohn IV component solid into 20mmol / L PBS solution (pH7.5), according to the ratio of 1:3.5 (w / v) After dissolving, use a magnetic stirrer to mix evenly to obtain the first complex solution.

[0074] (2) The first centrifugation: centrifuge the first complex solution at 10,000 rpm at 4° C. to obtain the first supernatant.

[0075] (3) Filtration for the first time: filter the supernatant for the first time with a 0.45 μm filter membrane to obtain the filtrate for the first time.

[0076] (4) pH adjustment: adjust the pH value of the first filtrate to 7.0 with 1 mol / L sodium citrate.

[0077] (5) Remove impurity protein: Add 15% (...

Embodiment 2

[0096] This embodiment is the combined use of ceruloplasmin and transferrin.

[0097] For the same experimental animal, in the same injection of the ceruloplasmin+transferrin composition, the mass ratio of ceruloplasmin to transferrin is 1:1. Among them, the activity of ceruloplasmin is 1.02unit / mg, and the purity of transferrin is 95%.

[0098] The aforementioned transferrin is the sterilized transferrin (TF solution) obtained in Step S4 of Example 1. The above-mentioned ceruloplasmin is obtained with reference to the method on pages 17-32 of the above-mentioned thesis. The pig serum in 3.1.5.1 is replaced with the serum of a healthy person to obtain a dialysate; described), hydroxyapatite adsorption chromatography (as described in 3.1.5.3), and Superdex-200 gel filtration chromatography (as described in 3.1.5.4), the component with the highest specific activity was used as ceruloplasmin Pure. Then, the pure ceruloplasmin was sterilized by filtration, the filter membrane w...

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Abstract

The invention belongs to the field of an application of biology drugs, and relates to an application of union of covelline albumen and transferrin to preparation of drugs for preventing and / or improving or / and treating Alzheimer's diseases, or an application to preparation of an intelligence benefitting replenisher or drugs, and a composition containing covelline albumen and transferrin. The covelline albumen participating regulation of metal ions in bodies balanced in plasma can improve self-care ability and learning and memory capacity of an animal dementia model by using transferrin, and the effects of improving memory and improving cognitive function can be achieved. The invention further provides a preparation method of the transferrin. According to the method, the transferrin can beextracted from waste Cohn IV components during production of blood products by a common Nitschmann-Kistler or cohn 6 method or cohn6+9 method at current, so that the limited plasma can be sufficientlyand reasonably used, and the waste of plasma resources can be reduced. The preparation method is simple, industrial scaled production can be directly realized, and the biology drugs can be applied topharmaceutical industry on a large scale.

Description

technical field [0001] The invention belongs to the application field of biopharmaceuticals, and the application of ceruloplasmin combined with transferrin in the preparation of drugs for preventing or / and improving or / and treating Alzheimer's disease; or / and in the preparation of intelligence supplements or drugs and compositions comprising ceruloplasmin and transferrin. Background technique [0002] Alzheimer's disease (Alzheimer's disease, AD), also known as senile dementia, is a degenerative disease of the brain and central nervous system caused by neuron loss, and the incidence of the disease in the elderly population is about 4%-8%. . In 1906, the German neuropathologist Alzheimer first reported a 51-year-old female patient with progressive memory loss. In 1910, psychiatrist Emil Kraepelin, known for naming and categorizing brain disorders, proposed the name Alzheimer's disease. The common clinical manifestations of the disease are progressive memory and cognitive d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/40A61P25/28C07K14/79C07K14/795C07K1/36C07K1/34C07K1/30C07K1/20A61K38/17
CPCA61K38/40A61K38/1709A61P25/28C07K14/79C07K14/795A61K2300/00
Inventor 栗琳崔雅轩谢蓝张丽丽娄玉霞管书丽石浩威
Owner 北京豪思生物科技股份有限公司
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