Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of romidepsin in preparation of medicine for preventing and treating novel coronavirus of COVID-19

A technology of romidepsin and coronavirus, applied in the direction of antiviral agents, drug combinations, pharmaceutical formulas, etc., can solve the problems of poor curative effect, achieve low effective concentration, high therapeutic index, and increase the effect

Pending Publication Date: 2021-12-21
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current treatment methods are symptomatic treatment, especially for some severe patients with poor curative effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of romidepsin in preparation of medicine for preventing and treating novel coronavirus of COVID-19

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Virus amplification

[0026] African green monkey kidney cells (VeroE6) were divided into 3×10 5 Each well was inoculated into a 96-well plate in Eagle’s minimum basal medium (minimum Eagle’s medium, MEM; GibcoInvitrogen) containing 10% fetal bovine serum (FBS; GibcoInvitrogen) at 37°C, 5% CO 2 Cultivate until the monolayer grows. Inoculate a 100-fold dilution of the clinical isolate of the novel coronavirus pneumonia into a 96-well plate full of monolayer cells, place at 37°C, 5% CO 2 Culture for two days (including the normal control group).

[0027] Two days later, the lesion degree reached more than 75%. Put it in a -80°C ultra-low temperature refrigerator, freeze and thaw once repeatedly, collect the virus liquid amplified by the cells, centrifuge at 3000r / min for 30 minutes, remove the sediment, put it in a small tube and put it in a -80°C ultra-low temperature freezer Long-term preservation.

Embodiment 2

[0028] Embodiment 2 Romidepsine Drug Toxicity Evaluation

[0029] After dissolving romidepsin powder in DMSO, add culture medium to dilute to 20 mg / mL, the final concentration of DMSO is 1%, filter through a 0.22 μm filter membrane and store at 4°C; filter and store at 4°C. Press about 2.5×10 per hole 4 Cells were seeded into a 96-well plate, and after 24 to 48 hours, when the cells had grown into a single layer, the culture medium was discarded, and 100 μL / well of drugs of different dilutions were added, and 100 μL / well of MEM was added to the control wells of normal cells, at 37°C in 5% CO 2 Continue culturing for 2 to 5 days, add 20 μL of CCK8 solution (5 mg / mL) to each well, and place at 37°C in 5% CO 2 Continue to incubate for 4 hours in the incubator. Discard the culture supernatant, add 100 μL dimethyl sulfoxide (DMSO) to each well, and shake at low speed for 10 minutes to fully dissolve the crystals. Select a wavelength of 490nm, and measure the light absorption val...

Embodiment 3

[0030] Example 3 Romidepsin anti-new coronary pneumonia novel coronavirus efficacy evaluation

[0031] To evaluate the antiviral efficacy of the drug, VeroE6 cells were grown at a density of 5×10 4 Cells / well were cultured overnight in a 48-well cell culture dish. Add virus (MOI 0.05) to infect for 2 hours. Then add 2-fold serial dilutions of the drug, set 4 replicate wells for each concentration, take the maximum non-toxic concentration as the initial concentration of the drug, at 34°C, 5% CO 2Incubate for 2 days in the incubator. Cytopathogenic Effect (CPE) was recorded. The occurrence of CPE in cells is recorded according to the 6-level standard. After recording the CPE, staining with CCK8, the OD value was determined. Efficacy evaluation of romidepsin against novel coronavirus pneumonia The efficacy of romidepsin was evaluated by the CCK8 method, and the half effective concentration (EC) against novel coronavirus pneumonia (SARS-Cov-2) 50 ) is 200nM, and the half eff...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of romidepsin in preparation of a medicine for preventing and treating novel coronavirus of COVID-19, and particularly discloses application of romidepsin or pharmaceutically acceptable salts, isotopes, stereoisomers, mixtures of stereoisomers, tautomers, esters, amides or prodrugs of romidepsin in preparation of medicine for preventing and / or treating diseases caused by coronavirus. The coronavirus is a novel coronavirus SARS-Cov-2, SARS-CoV, HCoV 229E, NL63, OC43, HKU1 and MERS-CoV. The half effective concentration of the romidepsin to the novel coronavirus of COVID-19 is 200nM, and the half effective concentration is low, so that the antiviral effect is good.

Description

technical field [0001] The invention belongs to the field of antiviral drugs, and in particular relates to the application of romidepsin in the preparation of drugs for preventing and treating novel coronaviruses against new coronary pneumonia. Background technique [0002] Novel coronavirus pneumonia (Corona Virus Disease 2019) is an infectious disease caused by a new type of coronavirus (SARS-Cov-2) infecting the human body. , 2020, 395(10223):507-513; The Lancet, 2020, 395(10223):497-506]. Coronaviruses belong to the genus Coronavirus in the family Coronaviridae. Viruses of the genus Coronavirus are positive-strand single-stranded RNA viruses with an envelope (envelope), with a diameter of about 80-120nm. Its genetic material is the largest among all RNA viruses, and generally only infects humans, mice, pigs, cats, and dogs. , Avian vertebrates. Coronaviruses were first isolated from chickens in 1937. The shape of coronavirus particles is irregular, with a diameter of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/15A61K45/06A61P31/14A61P11/00
CPCA61K38/15A61K45/06A61P31/14A61P11/00A61K2300/00
Inventor 袁曙光陈显翀罗木鹏邹荣峰崔文强赵金存孙静
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com